Functional Glycan Library for Analysis of Sugar-Protein Interaction Based on Glycoblotting Technology

• Project/Area Number: 19750132
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Chemistry related to living body
• Research Institution: Hokkaido University
• Principal Investigator: FURUKAWA Jun-ichi Hokkaido University, 大学院先端生命科学研究院, 特任助教授 (30374193)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,450,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥150,000); Fiscal Year 2008: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2007: ¥2,800,000 (Direct Cost: ¥2,800,000)
• Keywords: グライコミクス / ケミカルバイオロジー / 糖鎖認識分子 / 質量分析

Research Abstract

本研究ではこのようなアミノオキシ基もしくはヒドラジド基による糖鎖選択的な反応をさらに発展させ、混合物中に存在する糖鎖を迅速かつ大量に精製する技術を確立し、さらにはシークエンシャルな糖鎖タグの付け替えにより目的に応じた糖鎖ライブラリーを調製する新たな手法の開発を行う。

Research Products

• [Journal Article] Glycosylation specific for adhesion molecules in epidermis and its receptor revealed by glycoform-focused reverse genomics (2009)
  • Author(s): Uematsu R., Shinohara Y., Nakagawa H., Kurogochi M., Furukawa J.-I, Miura Y., Akiyama M., Shimizu H., Nishimura S.-I.
  • Journal Title: Mol. Cell. Proteomics 8 Pages: 232-244
  • Peer Reviewed
• [Journal Article] Glycosylation specific for adhesion molecules in epidermis and its receptor revealed by glycoform-focused reverse genomics (2009)
  • Author(s): R. Uematsu, Y. Shinohara, H. Nakagawa, M. Kurogochi, J.-i. Furukawa, Y. Miura, M. Akivama, H. Shimizu, S.-I. Nishimura
  • Journal Title: Mol. Cell. Proteom 8 Pages: 232-244
  • Peer Reviewed
• [Journal Article] Crucial Role of Aspartic Acid at Position 265 in the CH2 Domain for Murine IgG2a and IgG2b Fc-associated Effector Functions (2008)
  • Author(s): Baudino L., Shinohara Y., Nimmerjahn F., Furukawa J.-I., Nakata M., Martinez-Soria E., Petry F., Ravetch V. J., Nishimura S.-I., and Izui S.
  • Journal Title: J. Immunol. 181 Pages: 6664-6669
  • Peer Reviewed
• [Journal Article] Impact of a Three Amino-Acid Deletion in the CH2 Domain of Murine IgG1 on Fc-associated Effector Functions (2008)
  • Author(s): Baudino L., Nimmerjahn F., Shinohara Y., Furukawa J.-I., Petry F., Verbeek S., Nishimura S.-I., Ravetch V. J., and Izui S.
  • Journal Title: J. Immunol. 181 Pages: 4107-4112
  • Peer Reviewed
• [Journal Article] A comprehensive approach to structural and functional glycomics based on chemoselective glycoblotting and sequential tag conversion (2008)
  • Author(s): Furukawa J-I., Shinohara Y., Kuramoto H., Miura Y., Shimaoka H., Kurogochi M., Nakano M., Nishimura S.-I.
  • Journal Title: Anal. Chem. 4 Pages: 1094-1101
  • Peer Reviewed
• [Journal Article] Crucial Role of Aspartic Acid at Position 265 in the CH2 Domain for Murine IgG2a and IgG2b Fc-associated Effec tor Functions (2008)
  • Author(s): L. Baudino, Y. Shinohara, F. Nimmerjahn, J.-i. Furukawa, M. Nakata, E. Martinez-Soria, F. Petry, V. J Ravetch., S.-I. Nishimura, and S. Izui
  • Journal Title: J. Immunol 181 Pages: 6664-6669
  • Peer Reviewed
• [Journal Article] Impact of a Three Amino Acid Deletion in the CH2 Domain of Murine IgG1 on Fc-Associated Effector Functions (2008)
  • Author(s): L. Baudino, F. Nimmerjahn, Y. Shinohara, J.-i. Furukawa, F. Petry, J. S. Verbeek, S.-I. Nishimura, J. V. Ravetch, S. Izui
  • Journal Title: J. Immunol 181 Pages: 4107-4112
  • Peer Reviewed
• [Journal Article] Comprehensive approach to structural and functional glycomics based on chemoselective glycoblotting and sequential tag conversion (2008)
  • Author(s): Furukawa J.i., 他
  • Journal Title: Analytical Chemistry 80 Pages: 1094-1101
  • Peer Reviewed
• [Presentation] Glycoblotting法と連続的な標識交換に基づく構造および機能グライコミクスのための包括的なアプローチ (2007)
  • Author(s): 古川 潤一、篠原康郎、倉本洋光、三浦嘉晃、島岡秀行、黒河内正樹、中野美佳、岩崎倫政、三浪明男、西村紳一郎
  • Organizer: 日本糖質学会
  • Place of Presentation: 福岡