Development of fluorescent sensors for intracellular transition metal ions and their applications to ratiometric imaging

• Project/Area Number: 19750139
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Chemistry related to living body
• Research Institution: Kyoto University
• Principal Investigator: TAKI Masayasu Kyoto University, 大学院・地球環境学堂, 助教 (70378850)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥240,000); Fiscal Year 2008: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2007: ¥2,600,000 (Direct Cost: ¥2,600,000)
• Keywords: 生物無機化学 / 化学センサー / 金属イオンセンサー / 蛍光 / イメージング / レシオ測定 / カドミウムイオン / 水銀イオン / 銀イオン / 蛍光プローブ / レシオ型 / 選択性

Research Abstract

生体内における金属イオンの動的挙動を解析するためのツール分子として種々のセンサー分子の開発を行った。その結果、銀、カドミウム、亜鉛、および水銀などの金属イオンに対して特異的な応答を示す分子の開発に成功した。中でもカドミウムイオンセンサーを用いることで、レシオ法による細胞内カドミウムの濃度変化を世界で初めてイメージングすることに成功した。

Research Products

• [Journal Article] Development of ratiometric fluorescent probe for zinc ion based on indole fluoronhore (2009)
  • Author(s): M. Taki, Y. Watanabe, Y. Yamamoto
  • Journal Title: Tetrahedron Letters 50 Pages: 1345-1347
  • Peer Reviewed
• [Journal Article] A Rosamine-based Fluorescent Chemosensor for Selective Detection of Silver(I) in Aqueous Solution (2008)
  • Author(s): Shohei Iyoshi, Masayasu Taki, Yukio Yamamoto
  • Journal Title: Inorganic Chemistry 47 Pages: 3946-3948
  • Peer Reviewed
• [Journal Article] Effects on Heteroatom Substitution on the Structures, Physicochemical Properties, and Redox Behavior of Nickel(II) Complexes with Pyridine-Containing Macrocyclic Ligands (2008)
  • Author(s): Masayasu Taki, Yoshiaki Kawashima, Naoko Sakai, Tasuku Hirayama, Yukio Yamamoto
  • Journal Title: Bulletin of the Chemical Society of Japan 81 Pages: 590-597
  • NAID: 10021075440
  • Peer Reviewed
• [Journal Article] Colorimetric Response to Mercury -induced Abstraction of Triethylene Glycol Ligand from Gold Nanoparticle Surface (2008)
  • Author(s): Tasuku Hirayama, Masayasu Taki, Yukiyasu Kashiwagi, Masami Nakamoto, Atsushi Kunishita, Shinobu Itoh, Yukio Yamamoto
  • Journal Title: Dalton Transactions Pages: 4705-4707
  • Peer Reviewed
• [Journal Article] Fluorescence Imaging of Intracellular Cadmium Using a Dual-Excitation Ratiometric Chemosensor (2008)
  • Author(s): Masayasu Taki, Mika Desaki, Akio Ojida, Shohei Iyoshi, Tasuku Hirayama, Itaru Hamachi, Yukio Yamamoto
  • Journal Title: Journal of the American Chemical Society 130 Pages: 12564-12565
  • Peer Reviewed
• [Journal Article] A Rosamine-based Fluorescent Chemosensor for Selective Detection of Silver(I) in Aqueous Solution (2008)
  • Author(s): S. Iyoshi, M. Taki, Y. Yamamoto
  • Journal Title: Inorganic Chemistry 47 Pages: 3946-3948
  • Peer Reviewed
• [Journal Article] Effects on Heteroatom Substitution on the Structures, Physicochemical Properties, and Redox Behavior of Nickel(II) Complexes with Pyridine-Containing Macrocyclic Ligands (2008)
  • Author(s): M. Taki, Y. Kawashima, N. Sakai, T. Hiravama Y. Yamamoto
  • Journal Title: Bulletin of the Chemical Society of Japan 81 Pages: 590-597
  • NAID: 10021075440
  • Peer Reviewed
• [Journal Article] Colorimetric Response to Mercury -induced Abstraction of Triethylene Glycol Ligand from Gold Nanonarticle Surface (2008)
  • Author(s): T. Hirayama, M. Taki, Y. Kashiwagi, M. Nakamoto, A. Kunishita, S. Itoh, Y. Yamamoto
  • Journal Title: Dalton Transactions Pages: 4705-4707
  • Peer Reviewed
• [Journal Article] Fluorescence Imaging of Intracellular Cadmium Using a Dual-Excitation Ratiometric Chemosensor (2008)
  • Author(s): M. Taki, M. Desaki, A. Ojida, S. Iyoshi, T. Hirayama, I. Hamachi, Y. Yamamoto
  • Journal Title: Journal of the American Chemical Society 130 Pages: 12564-12565
  • Peer Reviewed
• [Journal Article] Synthesis of a new bifunctionalised fluorescent label and physical properties of the bound form on model peptide of troponin C (2007)
  • Author(s): Tasuku Hirayama, Syohei Iyoshi, Masa Taki, Yuichiro Maeda, Yukio Yamamoto
  • Journal Title: Organic & Biomolecular Chemistry 5 Pages: 2040-2045
  • Peer Reviewed
• [Journal Article] Synthesis of a new bifunctionalised fluorescent label and physical properties of the bound form on model (2007)
  • Author(s): T. Hirayama, S. Iyoshi, M. Taki, Y. Maeda, Y. Yamamoto
  • Journal Title: Organic & Biomolecular Chemistry 5 Pages: 2040-2045
  • Peer Reviewed
• [Presentation] ピリジン環含有環状ニッケル錯体と外部配位子との結合挙動 (2009)
  • Author(s): 旭史悦・多喜正泰・山本行男
  • Organizer: 日本化学会第89春季年会
  • Place of Presentation: 日本大学
  • Year and Date: 2009-03-29
• [Presentation] チオエーテル系多座配位子による銅-価酸化還元挙動の制御および隣接蛍光画の発光挙動に与える影響 (2009)
  • Author(s): 伊吉祥平・多喜正泰・山本行男
  • Organizer: 日本化学会第89春季年会
  • Place of Presentation: 日本大学
  • Year and Date: 2009-03-29
• [Presentation] ヌンチャク型ペプチドを利用した希土類亜鉛蛍光センサーの開発 (2009)
  • Author(s): 平山祐・多喜正泰・山本行男
  • Organizer: 日本化学会第89春季年会
  • Place of Presentation: 日本大学
  • Year and Date: 2009-03-28
• [Presentation] Ratiometric Fluorescence Imaging of Intracellular Cadmium Using a Coumarin-based Chemocensor (2008)
  • Author(s): S. lyoshi, M. Taki, A. Ojida, I. Hamachi, Y Yamamoto
  • Organizer: The 4th Asian Biological Inorganic Chemistry Conference
  • Place of Presentation: Jeju, Korea
  • Year and Date: 2008-11-12
• [Presentation] チオエーテル含有多座配位子を用いた銀および銅(I)錯体の構造と発光特性 (2008)
  • Author(s): 伊吉祥平・多喜正泰・山本行男
  • Organizer: 第58回錯体化学討論会
  • Place of Presentation: 金沢大学
  • Year and Date: 2008-09-21
• [Presentation] チオエーテル含有多座配位子を用いた銀および銅(1)錯体の構造と発光特性 (2008)
  • Author(s): 伊吉祥平・多喜正泰・山本行男
  • Organizer: 第58回錯体化学討論会
  • Place of Presentation: 金沢大学
  • Year and Date: 2008-09-20
• [Presentation] 新規ピリジン含有環状ニッケル錯体の構造と物性および外部配位子との挙動 (2008)
  • Author(s): 旭史悦・多喜正泰・山本行男
  • Organizer: 第58回錯体化学討論会
  • Place of Presentation: 金尺大学
  • Year and Date: 2008-09-19
• [Presentation] 外部配位子との配位挙動に及ぼす環状ニッケル錯体の置換基効果 (2008)
  • Author(s): 若松俊彦・多喜正泰・山本行男
  • Organizer: 日本化学会第88春季年会
  • Place of Presentation: 立教大学
  • Year and Date: 2008-03-30
• [Presentation] タンパク質の可逆的蛍光ラベル化を指向した二官能基性ローダミン分子の開発 (2008)
  • Author(s): 春木秀仁・平山祐・多喜正泰・山本行男
  • Organizer: 日本化学会第88春季年会
  • Place of Presentation: 立教大学
  • Year and Date: 2008-03-28
• [Presentation] エチレングリコール担持金クラスターによる水銀イオンの特異的検出とその反応機構 (2008)
  • Author(s): 平山祐・多喜正泰・山本行男
  • Organizer: 日本化学会第88春季年会
  • Place of Presentation: 立教大学
  • Year and Date: 2008-03-28
• [Presentation] チオエーテル配位子を有するロサミン型蛍光センサーの構造と機能 (2008)
  • Author(s): 伊吉祥平・多喜正泰・山本行男
  • Organizer: 日本化学会第88春季年会
  • Place of Presentation: 立教大学
  • Year and Date: 2008-03-27
• [Presentation] Ratiometric Fluorescence Imaging of Intracellular Cadmium Using a Coumarin-based Chemosensor (2008)
  • Author(s): Shohei Iyoshi, Masayasu Taki, Akio Ojida, Itaru Hamachi, Yukio Yamamoto
  • Organizer: The 4th Asian Biological Inorganic Chemistry Conference
  • Place of Presentation: Jeju, Korea
• [Presentation] Membrane-permeable Fluorescent Chemo-sensor for Ratiometric Imaging of Cadmium in Living Cells (2007)
  • Author(s): Masaasu Taki and Yukio Yamamoto
  • Organizer: Biomimetics Conference
  • Place of Presentation: Doshisha, Kyoto, Japan
  • Year and Date: 2007-12-20
• [Presentation] Membrane-permeable Fluorescent Chemosensor for Ratiometric Imaging of Cadmium in (2007)
  • Author(s): Masayasu Taki and Yukio Yamamto
  • Organizer: Biomimetics Conference
  • Place of Presentation: Doshisha University
  • Year and Date: 2007-12-08
• [Presentation] Development of New Lanthanide Complexes Binding to Peptide Tag (2007)
  • Author(s): 〓suku Hirayama, Masayasu Taki, Yukio Yamamoto
  • Organizer: Biomimetics Conference
  • Place of Presentation: Doshisha University
  • Year and Date: 2007-12-08
• [Presentation] アミノクマリンを骨格とした新規レシオ型カドミウム蛍光プローブの開発 (2007)
  • Author(s): 多喜正泰・出崎美佳・山本行男
  • Organizer: 第22回生体機能関連化学シンポジウム
  • Place of Presentation: 東北大学
  • Year and Date: 2007-09-28
• [Presentation] 新しいペプチドタグ結合性希土類錯体の開発 (2007)
  • Author(s): 平山祐・多喜正泰・山本行男
  • Organizer: 第22回生体機能関連化学シンポジウム
  • Place of Presentation: 東北大学
  • Year and Date: 2007-09-28
• [Presentation] エチレングリコール担持金クラスターを用いた水銀の特異的検出とその反応メカニズム (2007)
  • Author(s): 平山祐・多喜正泰・山本行男
  • Organizer: 第57回錯体化学討論会
  • Place of Presentation: 名古屋工業大学
  • Year and Date: 2007-09-26
• [Presentation] チオエーテル配位子を有するロサミン型蛍光プローブの開発と金属イオンに対する蛍光挙動 (2007)
  • Author(s): 伊吉祥平・多喜正泰・山本行男
  • Organizer: 第57回錯体化学討論会
  • Place of Presentation: 名古屋工業大学
  • Year and Date: 2007-09-26
• [Presentation] タンパク質蛍光ラベル化を目指したペプチド結合性二ッケル錯体の合成と性質 (2007)
  • Author(s): 若松俊彦・平山祐・多喜正泰・山本行男
  • Organizer: 第57回錯体化学討論会
  • Place of Presentation: 名古屋工業大学
  • Year and Date: 2007-09-26
• [Presentation] Highly Selective Ratiometric Fluorescent Probe with Picomolar sensitivity for Cadmium (2007)
  • Author(s): Masayasu Taki and Yukio Yamamoto
  • Organizer: 13th International Conference on Biological Inorganic Chemistry
  • Place of Presentation: University of Vienna
  • Year and Date: 2007-07-16
• [Presentation] Mechanism of colorimetric sensing of Hg2+ with triethyleneglycol-supported gold nano-cluster (2007)
  • Author(s): Tasuku Hirayama, Masayasu Taki, Yukio Yamamoto
  • Organizer: 13th International Conference on Biological Inorganic Chemistry
  • Place of Presentation: University of Vienna
  • Year and Date: 2007-07-16
• [Presentation] Highly Selective Ratiometric Fluorescent Probe with Picomolar sensitivity for Cadmium (2007)
  • Author(s): Masayasu Taki and Yukio Yamamoto
  • Organizer: 13th International Conference on Biological Inorganic Chemistry
  • Place of Presentation: Vienna, Austria
• [Remarks]
  • URL: http://www.users.kudpe.kyoto-u.ac/jp/~t50544/
• [Remarks]
  • URL: http://www.h.kyoto-u.acjp/staff/321_taki_m_O_j.html