Investigation on the reaction mechanism of heme oexygenase having non-natural tetrapyrrolic cofactors

• Project/Area Number: 19750142
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Chemistry related to living body
• Research Institution: Osaka University
• Principal Investigator: MATSUO Takashi Osaka University, 大学院工学研究科, 講師 (50432521)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,790,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥390,000); Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2007: ¥2,100,000 (Direct Cost: ¥2,100,000)
• Keywords: ヘムオキシゲナーゼ / ポルフィセン / 非天然補因子 / 速度論 / 酸化分解 / 血相構造解析 / テトラピロール / 還元速度 / 片足ヘム / 結晶構造解析

Research Abstract

生体内でヘム補因子の酸化分解に関与するヘムオキシゲナーゼについて、非天然合成ヘム類縁体を結合させたときの作用機構について、反応速度論および結晶構造解析によって検討した。その結果、補因子の化学構造および補因子とタンパク質の相互作用が、タンパク質機能に大きな役割を果たしていることが判明した。

Research Products

• [Journal Article] Photocatalytic Hydrogen Generation Using a Protein-Coated Photosensitizer with Anionic Patches and a Monocationic Electron Mediator (2008)
  • Author(s): Takashi Matsuo, Atsushi Asano, Tsutomu Ando, Yoshio Hisaeda, Takashi Hayashi
  • Journal Title: Chem. Commun. Pages: 3864-3686
• [Journal Article] Isolable Iron(II)-Porphycene Derivative Stabilized by Introduction of Trifluoromethyl Groups on the Ligand Framework (2008)
  • Author(s): Kazuyuki Ito, Takashi Matsuo, Isao Aritome, Yoshio Hisaeda, Takashi Hayashi
  • Journal Title: Bull. Chem. Soc. Jpn. 81 Pages: 76-83
  • NAID: 10020139404
• [Journal Article] Effect of Peripheral Trifluoromethyl Groups in Artificial Iron Porphycene Cofactor on Ligand Binding Properties of Myoglobin (2008)
  • Author(s): Takashi Matsuo, Kazuyuki Ito, Yuji Nakashima, Yoshio Hisaeda, Takashi Hayashi
  • Journal Title: J. Inorg. Biochem. Pages: 166-173
• [Journal Article] Photocatalytic Hydrogen Generation Using a Protein-Coated Photosensitizer with Anionic Patches and a Monocationic Electron Mediator (2008)
  • Author(s): Takashi Matsuo, Atsushi Asano, Tsutomu Ando, Yoshio Hisaeda, Takashi Hayashi
  • Journal Title: Chem. Commun 2008 Pages: 3864-3868
  • Peer Reviewed
• [Journal Article] Effect of Peripheral Trifluoromethyl Groups in Artificial Iron Porphycene Cofactor on Ligand Binding Properties of Myoglobin (2008)
  • Author(s): T. Matsuo, K. Ito, Y. NakashimaY. Hisaeda, T. Hayashi
  • Journal Title: J. Inorg. Biochem. 102 Pages: 166-173
  • Peer Reviewed
• [Journal Article] Isolable Iron(II)-Porphycene Derivative Stabilized by Introduction of Trifluoromethyl Groups on the Ligand Framework (2008)
  • Author(s): K. Ito, T. Matsuo, I. Aritome,Y. Hisaeda, T. Hayashi
  • Journal Title: Bull. Chem. Soc. Jpn. 81 Pages: 76-83
  • NAID: 10020139404
  • Peer Reviewed
• [Journal Article] Photocatalytic Hydrogen Generation Using a Protein-Coated Photosensitizer with Anionic Patches and a Monocationic Electron Mediator (2008)
  • Author(s): T. Matsuo, A. Asano, T. Ando, Y. Hisaeda, T. Hayashi
  • Journal Title: Chemical Commuications 31 Pages: 3684-3686
  • Peer Reviewed
• [Journal Article] A Structural Isomer of Nonarpmatic Porphyrin : Preparation of 20pi-Conjugated Porphycene Based on Electronic Perturbation (2007)
  • Author(s): Takashi Matsuo, Kazuyuki Ito, Nobuko, Kanehisa, Takashi Hayahi
  • Journal Title: Org. Lett. 9 Pages: 5303-5306
• [Journal Article] Porphyrinoid Chemistry in Hemoprotein Matrix : Detection and Reactivities of Iron(IV)-Oxo Species of Porphycene Incorporated into Horseradish Peroxidase (2007)
  • Author(s): Takashi Matsuo, Dai Murata, Yoshio Hisaeda, Hiroshi Hori, Takashi Hayashi
  • Journal Title: J. Am. Chem. Soc. 129 Pages: 12906-12907
• [Journal Article] Porphyrinoid Chemistry in Hemoprotein Matrix : Detection and Reactivities of Iron(IV)-Oxo Species of Porphycene Incorporated into Horseradish Peroxidase (2007)
  • Author(s): T. Matsuo, D. Murata, Y. Hisaeda, H. Hori, T. Hayashi
  • Journal Title: J. Am. Chem. Soc. 129 Pages: 12906-12907
  • Peer Reviewed
• [Journal Article] A Structural Isomer of Nonaromatic Porphyrin : Preparation of 20π-Conjugated Porphycene Based on Electronic Perturbation (2007)
  • Author(s): T. Matsuo, K. Ito, N. Kanehisa, T. Hayashi
  • Journal Title: Org. Lett. 9 Pages: 5303-5306
  • Peer Reviewed
• [Journal Article] Structure and Ligand Binding Properties of Myoglobins Reconstituted with Monodepropionated Heme : Functional Role of Each Heme Propionateside Chain (2007)
  • Author(s): K. Harada, M. Makino, H. Sugimoto, S. Hirota, T. Matsuo, Y. Shiro, Y. Hisaeda. T. Hayashi
  • Journal Title: Biochemistry 46 Pages: 9406-9416
  • Peer Reviewed
• [Journal Article] Electron Transfer and Oxidation Activities of Reconstituted Hemoproteins with Chemically Modif Cofactor
  • Author(s): Takashi Matsuo,Takashi Hayashi
  • Journal Title: J. Porphyrins. Phthalocyanines. (in press)
• [Journal Article] Electron Transfer and Oxidation Activities in Reconstituted Hennouroteins with Chemically Modified Cofactor
  • Author(s): Takashi Matsuo, Takashi Hayashi
  • Journal Title: J. Porphyrins. Phthalocyanines (印刷中)
  • Peer Reviewed
• [Presentation] ヘムタンパク質中におけるポルフィセンおよびコロール鉄錯体の高酸 化状態の検出と反応性 (2008)
  • Author(s): 松尾貴史、藤井道子、阿部真人、林 高史
  • Organizer: 第58回錯体化学討論会
  • Place of Presentation: 金沢大学角間キャンパス
  • Year and Date: 2008-09-19
• [Presentation] ペムタンパク質中におけるポルフィセンおよびコロール鉄錯体の高酸化状態の検出と反応性 (2008)
  • Author(s): 松尾貴史、藤井道子・阿部真人・林高史
  • Organizer: 第58回錯体化学討論会
  • Place of Presentation: 金沢大学角間キャンパス
  • Year and Date: 2008-09-16
• [Presentation] Enhancement of Peroxidase Activity for Several Hemoproteins by Reconstitution with Artificially Created Prosthetic Groups (2008)
  • Author(s): Takashi Matsuo
  • Organizer: Fifth International Conference on Porphyrins and Phthalocyanines (ICPP-5)
  • Place of Presentation: ロシア科学アカデミー(モスクワ)
  • Year and Date: 2008-07-08
• [Presentation] Synthesis and Characterization of 20-pi Conjugated Porphycene with TrfluoromethylGroups (2008)
  • Author(s): Takashi Matsuo, Yuji Tohi, Kazuyuki Ito, Takashi Hayashi
  • Organizer: 5th International Conference on Porphyrins and Phthalocyanines
  • Place of Presentation: ロシア科学アカデミー
  • Year and Date: 2008-07-07
• [Presentation] Synthesis and Characterization of 20π-Conjugated Porphycene with Trifluoromethyl Groups (2008)
  • Author(s): Takashi Matsuo, Yuji Tohi, Kazuyuki Ito, Takashi Hayashi
  • Organizer: 5th International Conference on Porphyrins and Phthalocyanines
  • Place of Presentation: ロシア科学アカデミー
  • Year and Date: 2008-07-07
• [Presentation] 非天然テトラピロール錯体を補因子とするヘムタンパク質の酸化反応特性 (2008)
  • Author(s): 松尾貴史
  • Organizer: 第18回金属の関与する生体関連反応シンポジウム
  • Place of Presentation: 名古屋市立大学
  • Year and Date: 2008-06-06
• [Presentation] Reactivities of porphyrin analogs in hemoprotein coordination space (2007)
  • Author(s): Takashi Matsuo
  • Organizer: 3rd International Symposium on Chemistry of Coordination Space
  • Place of Presentation: 淡路夢舞台国際会議場
  • Year and Date: 2007-12-11
• [Presentation] 非天然テトラピロール系錯体を有する再構成ヘムタンパク質による酸化反応 (2007)
  • Author(s): 松尾貴史
  • Organizer: 第40回酸化反応討論会
  • Place of Presentation: 奈良女子大学
  • Year and Date: 2007-11-18
• [Presentation] Reactivities of high-valent species of heme analogs generated in hemoprotein matrix (2007)
  • Author(s): Takashi Matsuo
  • Organizer: 67^th Okazaki Conference "Molecular Science and Chemical Biology of Biomolecular Function
  • Place of Presentation: 岡崎コンファレンスセンター
  • Year and Date: 2007-11-11
• [Presentation] ヘムタンパク質マトリクス内におけるポリフィリノイド鉄錯体酸化活性種の反応性 (2007)
  • Author(s): 松尾貴史、藤井道子、阿部真人、村田大、林 高史
  • Organizer: 第22回生体機能関連化学シンポジウム
  • Place of Presentation: 東北大学片平キャンパス
  • Year and Date: 2007-09-30
• [Presentation] ヘムタンパク質マトリクス内におけるポリフィリノイド鉄錯体酸化活性種の反応性 (2007)
  • Author(s): 松尾貴史
  • Organizer: 第22回生体機能関連化学シンポジウム
  • Place of Presentation: 東北大学
  • Year and Date: 2007-09-30
• [Presentation] Reactivities of porphyrinoid incorporated into hemoprotein matrices (2007)
  • Author(s): Takashi Matsuo
  • Organizer: The first Asian Conference on Coordination Chemistry
  • Place of Presentation: 岡崎コンファレンスセンター
  • Year and Date: 2007-07-29
• [Presentation] Reactivities of Porphyrinoid Incorporated into Hemoprotein Matrices (2007)
  • Author(s): Takashi Matsuo, Dai Murata, Masato Abe, Michiko Fujii, Takashi Hayashi
  • Organizer: The first Asian Conference on Cooridination Chemistry
  • Place of Presentation: 岡崎コンファレンスセンター
  • Year and Date: 2007-07-16
• [Presentation] Photo-induced Electron Transfer of Reconstituted Myoglobin Having Anionic Domain and Application to Photocatalytic Hydrogen Evolution (2007)
  • Author(s): Atsushi Asano, Takashi Matsuo, Hayashi Takashi
  • Organizer: The first Asian Conference on Cooridination Chemistry
  • Place of Presentation: 岡崎コンファレンスセンター
  • Year and Date: 2007-07-16
• [Remarks]
  • URL: http://www.chem.eng.osaka-u.ac.jp/~hayashiken/matsuo_files/matsuo_jpn.html
• [Remarks]
  • URL: http://www.chem.eng.osaka-u.ac.jp/~hayashiken/matsuo_files/publication.html