細胞核内環境を再現するヒストン模倣ナノ粒子の作製とそれによる転写活性の制御

• Project/Area Number: 19750149
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Chemistry related to living body
• Research Institution: Konan University
• Principal Investigator: MIYOSHI Daisuke Konan University, 先端生命工学研究所, 講師 (50388758)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,750,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥450,000); Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2007: ¥1,800,000 (Direct Cost: ¥1,800,000)
• Keywords: ヒストン / 転写 / 核酸 / 分子クラウディング / ナノ粒子 / ワトソン‐クリック塩基対 / フーグスティーン塩基対 / クロマチン / ワトソン-クリック塩基対

Research Abstract

核酸の構造と熱力学的安定性に関して細胞核内を模倣した分子クラウディング条件下で検討した結果、分子クラウディングによって核酸の標準構造(二重らせん構造)が不安定化し、核酸の非標準構造は安定化した。さらに、細胞核内に存在するヒストンの核酸結合部位を化学的に合成し、核酸構造の安定性に及ぼす効果を検討したところ、ヒストン模倣ペプチドによって、核酸の非標準高次構造のみが安定化することが示された。また、これらの知見を用いることで、テロメラーゼなどの酵素機能も制御できた。

Research Products

• [Journal Article] Hydration of Watson-Crick base pairs and dehydration of Hoogsteen base pairs inducing structural polymorphism under molecular crowding conditions (2009)
  • Author(s): D. Miyoshi, K. Nakamura, H. Tateishi-Karimata, T. Ohmichi, and N. Sugimoto
  • Journal Title: J. Am. Chem. Soc 131 Pages: 3522-3531
  • Peer Reviewed
• [Journal Article] An approach to peptide-based ATP receptors by a combination of random selection, rational design, and molecular imprinting (2009)
  • Author(s): J. Matsui, J. Nagano, D. Miyoshi, K. Tamaki, N. Sugimoto
  • Journal Title: Biosens. Bioelectron (in press)
  • Peer Reviewed
• [Journal Article] 分子メタモルフォーゼ-環境に応答するDNA (2009)
  • Author(s): 三好大輔
  • Journal Title: 生命化学研究レター 29 Pages: 9-14
• [Journal Article] Hydration of Watson-Crick base pairs and dehydration of Hoogsteen base pairs inducing structural polymorphism under molecular crowding conditions (2009)
  • Author(s): D. Miyoshi, K. Nakamura, H. Karimata, T. Ohmichi, N. Sugimoto
  • Journal Title: J. Am. Chem. Soc 130 Pages: 3522-3531
  • Peer Reviewed
• [Journal Article] 分子メタモルフォーゼ-環境に応答するDNA- (2009)
  • Author(s): 三好大輔
  • Journal Title: 生命化学研究レター 29 Pages: 9-14
• [Journal Article] Thermodynamics of DNA structures under molecular crowding conditions with neutral and positive charged cosolutes (2008)
  • Author(s): D. Miyoshi, K. Nakamura, S. Muhuri, H. T. Karimata, and N. Sugimoto
  • Journal Title: Nucleic Acids Symp. Ser 52 Pages: 413-414
• [Journal Article] Telomerase activity regulated by thermodynamic stability of DNA?RNA hybrid (2008)
  • Author(s): H. Yu, D. Zhang, X. Gu, D. Miyoshi, and N. Sugimoto
  • Journal Title: Angew. Chem. Int. Ed 47 Pages: 9034-9038
  • Peer Reviewed
• [Journal Article] Molecular crowding effects on structure and stability of DNA (2008)
  • Author(s): D. Miyoshi and N. Sugimoto
  • Journal Title: Biochimie 90 Pages: 1040-1051
• [Journal Article] Cationic porphyrin induced a telomeric DNA to G-quadruplex form in water (2008)
  • Author(s): T. Murashima, D. Sakiyama, D. Miyoshi, M. Kuriyama, T. Yamada, T. Miyazawa, and N. Sugimoto
  • Journal Title: Bioinorg. Chem. Appl 294756
  • Peer Reviewed
• [Journal Article] Riboswitches for enhancing target gene expression in eukaryotes (2008)
  • Author(s): 6 T. Yamauchi, D. Miyoshi, T. Kubodera, M. Ban, A. Nishimura, and N. Sugimoto
  • Journal Title: ChemBioChem 9 Pages: 1040-1043
  • Peer Reviewed
• [Journal Article] Small-molecule-directed assembly of DNA : a gold-nanoparticle-based strategy for screening of a DNA duplex binders (2008)
  • Author(s): G. Song, C. Chen, X. Qu, D. Miyoshi, J. Ren, and N. Sugimoto
  • Journal Title: Adv. Mater 20 Pages: 706-710
  • Peer Reviewed
• [Journal Article] 外部刺激に応答する機能性核酸-核酸ならではの機能を活用したナノバイオマテリアルの設計- (2008)
  • Author(s): 三好大輔・杉本直己
  • Journal Title: 化学工業(化学工業社) 59(11) Pages: 21-27
• [Journal Article] Telomerase activity regulated by thermodynamic stability of DNA・RNA hybrid (2008)
  • Author(s): H. Yu, D. Zhang, X. Gu, D. Miyoshi, N. Sugimoto
  • Journal Title: Angew. Chem. Int. Ed 47 Pages: 9034-9038
  • Peer Reviewed
• [Journal Article] Riboswitches for enhancing target gene expression in eukaryotes (2008)
  • Author(s): T. Yamauchi, D. Miyoshi, T Kubodera, M. Ban, A. Nishimura, N. Sugimoto
  • Journal Title: ChemBioChem 9 Pages: 1040-1043
  • Peer Reviewed
• [Journal Article] Molecular crowding effects on structure and stability of DNA (2008)
  • Author(s): D. Miyoshi, N. Sugimoto
  • Journal Title: Biochimie 90 Pages: 1040-1051
  • Peer Reviewed
• [Journal Article] Thermodynamics of DNA structures under molecular crowding conditions with neutral and positive charged cosolutes (2008)
  • Author(s): D. Miyoshi, K. Nakamura, S. Muhuli, H. T. Karimata, N. Sugimoto
  • Journal Title: Nucleic Acids Symp. Ser 52 Pages: 413-414
• [Journal Article] 外部刺激に応答する機能性核酸-核酸ならではの機能を活用したナノバイオマテリアルの設計 (2008)
  • Author(s): 三好大輔・杉本直己
  • Journal Title: 化学工業 59 Pages: 21-27
• [Journal Article] Synthesis, structure and thermal stability of fully hydrophobic porphyrin-DNA conjugates (2007)
  • Author(s): T. Murashima, K. Hayata, Y. Saiki, J. Matsui, D. Miyoshi, T., Yamada, T. Miyazawa, and N. Sugimoto
  • Journal Title: Tetrahedron Lett 48 Pages: 8514-8517
  • Peer Reviewed
• [Journal Article] Effects of cosolutes on the thermodynamic stability of parallel DNA duplex and triplex (2007)
  • Author(s): K. Nakamura, H. Karimata, T. Ohmichi, D. Miyoshi, and N. Sugimoto
  • Journal Title: Nucleic Acids Symp. Ser 51 Pages: 167-168
• [Journal Article] Conformational switch of a functional nanowire based on the DNA G-quadruplex (2007)
  • Author(s): H. Karimata, D. Miyoshi, T. Fujimoto, K. Koumoto, Z.-M. Wang, and N. Sugimoto
  • Journal Title: Nucleic Acids Symp. Ser 51 Pages: 251-252
• [Journal Article] Hydration regulates thermodynamic stability of DNA structures under molecular crowding, conditions Nucleosides (2007)
  • Author(s): D. Miyoshi, H. Karimata, and N. Sugimoto
  • Journal Title: Nucleotides and Nucleic Acids 26 Pages: 589-595
  • Peer Reviewed
• [Journal Article] Regulation of DNA nucleases by molecular crowding (2007)
  • Author(s): Y. Sasaki, D. Miyoshi, and N. Sugimoto
  • Journal Title: Nucleic Acids Res 35 Pages: 4086-4093
  • Peer Reviewed
• [Journal Article] Artificial G-wire Switch with 2,2'-Bipyridine Units Responsive to Divalent Metal Ions (2007)
  • Author(s): D. Miyoshi, H. Karimata, Z.-M. Wang, K. Koumoto, N. Sugimoto
  • Journal Title: J. Am. Chem. Soc 129 Pages: 5919-5925
  • Peer Reviewed
• [Journal Article] Molecular Imprinting Under Molecular Crowding Conditions : an Aid to the synthesis of a High-capacity Polymeric Sorbent for Triazine Herbicides (2007)
  • Author(s): J. Matsui, S. Goji, T. Murashima, D. Miyoshi, S. Komai, A. Shigeyasu, T. Kushida, T. Miyazawa, T. Yamada, K. Tamaki, and N. Sugimoto
  • Journal Title: Anal. Chem 79 Pages: 1749-1757
  • Peer Reviewed
• [Journal Article] DNA四重らせん構造を細胞内環境因子で制御する (2007)
  • Author(s): 三好大輔
  • Journal Title: 生体機能関連化学部会News Letter 22(3) Pages: 14-17
• [Journal Article] DNAデザイン-機能性分子開発への道しるべ- (2007)
  • Author(s): 杉本直己・三好大輔
  • Journal Title: 化学 62(9) Pages: 28-32
  • NAID: 40015605700
• [Journal Article] DNAを用いたバイオ計測-センシング核酸の開発と新展開 (2007)
  • Author(s): 三好大輔・杉本直己
  • Journal Title: 未来材料 7(6) Pages: 10-18
• [Journal Article] Artificial G-wire switch with 2,2'-bipyridine units responsive to divalent metal ions. (2007)
  • Author(s): D. Miyoshi, H. Karimata, Z.-M. Wang, K. Koumoto, and N. Sugimoto
  • Journal Title: J. Am. Chem, Soc. 129 Pages: 5919-5925
  • Peer Reviewed
• [Journal Article] Molecular imprinting under molecular crowding conditions: an aid to the synthesis of a high-capacity polymeric sorbent for triazine herbicides. (2007)
  • Author(s): J. Matsui, S. Goji, T. Murashima, D. Miyoshi, S. Komai, A. Shigeyasu, T. Kushida, T. T, Miyazawa, T. Yamada, K. Tamaki, and N. Sugimoto
  • Journal Title: Anal. Chem. 79 Pages: 1749-1757
  • Peer Reviewed
• [Journal Article] Synthesis, structure and thermal stability of fully hydrophobic porphyrin-DNA conjugates. (2007)
  • Author(s): T. Murashima, K. Hayata, Y. Saiki, J. Matsui, D. Miyoshi, T. Yamada, T. Miyazawa, and N. Sugimoto
  • Journal Title: Tetrahedron Lett. 48 Pages: 8514-8517
  • Peer Reviewed
• [Journal Article] Nucleosides, Nucleotides and Nucleic Acids (2007)
  • Author(s): D. Miyoshi, H. Karimata, and N. SugimotoHydration regulates thermodynamic stability of DNA structures under molecular crowding conditions.
  • Journal Title: Nucleosides, Nucleotides and Nucleic Acids 26 Pages: 589-595
  • Peer Reviewed
• [Journal Article] Regulation of DNA nucleases by molecular crowding. (2007)
  • Author(s): Y. Sasaki, D. Miyoshi, and N. Sugimoto
  • Journal Title: Nucleic Acids Res. 35 Pages: 4086-4093
  • Peer Reviewed
• [Journal Article] Effects of cosolutes on the thermodynamic stability of parallel DNA duplex and triplex. (2007)
  • Author(s): K. Nakamura, H. Karimata, T. Ohmichi, D. Miyoshi, and N. Sugimoto
  • Journal Title: Nucleic Acids Symp. Ser. 51 Pages: 167-168
• [Journal Article] Conformational switch of a functional nanowire based on the DNA G-quadruplex. (2007)
  • Author(s): H. Karimata, D. Miyoshi, T. Fujimoto, K. Koumoto, Z.-M. Wang, and N. Sugimoto
  • Journal Title: Nucleic Acids Symp. Ser. 51 Pages: 251-252
• [Presentation] NanoBioNow(19)テロメアDNAの構造安定性とテロメラーゼ活性の相関 (2009)
  • Author(s): 三村健太、Hai-Quirlg Yu、Dong-Hao Zhang、三好大輔、杉本直己
  • Organizer: 日本化学会第89春季年会
  • Place of Presentation: 千葉
  • Year and Date: 2009-03-30
• [Presentation] NanoBioNow(20) DNA四重鎖構造の熱力学的安定性に及ぼすループ領域の役割 (2009)
  • Author(s): 藤本健史、三好大輔、杉本直己
  • Organizer: 日本化学会第89春季年会
  • Place of Presentation: 千葉
  • Year and Date: 2009-03-30
• [Presentation] NanoBioNow(21) DNAの構造安定性に及ぼすピストン模倣ペプチドの効果 (2009)
  • Author(s): 三好大輔、中村かおり、Muhuri Sanjukta、杉本直己
  • Organizer: 日本化学会第89春季年会
  • Place of Presentation: 千葉
  • Year and Date: 2009-03-30
• [Presentation] NanoBioNow(17)麹菌由来リボスイッチの機能改変 (2009)
  • Author(s): 山内隆寛、三好大輔、徳井美里、神谷久弥、松永將義、杉本直己
  • Organizer: 日本化学会第89春季年会
  • Place of Presentation: 千葉
  • Year and Date: 2009-03-29
• [Presentation] NanoBioNow(23)紫外線照射サケ白子DNAの紫外線吸収スペクトル (2009)
  • Author(s): 佐々木義晴、三好大輔、杉本直己
  • Organizer: 日本化学会第89春季年会
  • Place of Presentation: 千葉
  • Year and Date: 2009-03-29
• [Presentation] NanoBioNow(23)紫外線照射サケ白子DNAの紫外線吸収スペクトル (2009)
  • Author(s): 佐々木義晴・三好大輔・杉本直己
  • Organizer: 日本化学会第89回春季年会
  • Place of Presentation: 千葉
• [Presentation] NanoBioNow(21)DNAの構造安定性に及ぼすヒストン模倣ペプチドの効果 (2009)
  • Author(s): 三好大輔・中村かおり・Muhuri Sanjukta・杉本直己
  • Organizer: 日本化学会第89回春季年会
  • Place of Presentation: 千葉
• [Presentation] NanoBioNow(20)DNA四重鎖構造の熱力学的安定性に及ぼすループ領域の役割 (2009)
  • Author(s): 藤本健史・三好大輔・杉本直己
  • Organizer: 日本化学会第89回春季年会
  • Place of Presentation: 千葉
• [Presentation] NanoBioNow(19)テロメアDNAの構造安定性とテロメラーゼ活性の相関 (2009)
  • Author(s): 三村健太・Yu Hai-Quing・Zhang Dong-Hao・三好大輔・杉本直己
  • Organizer: 日本化学会第89回春季年会
  • Place of Presentation: 千葉
• [Presentation] NanoBioNow(17)麹菌由来リボスイッチの機能改変 (2009)
  • Author(s): 山内隆寛・三好大輔・徳井美里・神谷久弥・松永將義・杉本直己
  • Organizer: 日本化学会第89回春季年会
  • Place of Presentation: 千葉
• [Presentation] Artificial DNA forming metal-responding nanowire (2008)
  • Author(s): D. Miyoshi, H. T. Karimata, Z. -M. Wang, K. Koumoto, N. Suaimoto
  • Organizer: RSC Conference; Chemistry in the New World of Bioengineering and Synthetic Biology
  • Place of Presentation: Oxford(イギリス)
  • Year and Date: 2008-09-23
• [Presentation] DNA四重鎖の構造形成におけるカチオンと水分子の役割 (2008)
  • Author(s): 藤本健史・三好大輔・三村健太・狩俣寿枝・杉本直己
  • Organizer: 第3回バイオ関連化学合同シンポジウム
  • Place of Presentation: 横浜
  • Year and Date: 2008-09-19
• [Presentation] 核酸の構造安定性に及ぼす分子クラウディングとヒストン模倣ペプチドの影響 (2008)
  • Author(s): 三好大輔・中村かおり・Muhuri Sanjukta・狩俣寿枝・杉本直己
  • Organizer: 第3回バイオ関連化学合同シンポジウム
  • Place of Presentation: 横浜
  • Year and Date: 2008-09-19
• [Presentation] 分子クラウディング環境下におけるテロメラーゼ活性の検討 (2008)
  • Author(s): Zhang Dong-Hao・Yu Hai-Qing, 三好大輔, ・杉本直己
  • Organizer: 第3回バイオ関連化学合同シンポジウム
  • Place of Presentation: 横浜
  • Year and Date: 2008-09-18
• [Presentation] Thermodynamics of DNA structures under molecular crowding conditions with neutral and positive charged cosolutes (2008)
  • Author(s): D. Miyoshi, K. Nakamura, S. Muhuli, H. T. Karimata, N. Sugimoto
  • Organizer: Joint Symposium of the 18th International Roun dtable on Nucleosides, nucleotides and Nucleic Acids and the 35th International Symposium on Nucleic Acids Chemistry
  • Place of Presentation: 京都
  • Year and Date: 2008-09-11
• [Presentation] DNA nanostructures that respond to chemical stimuli (2008)
  • Author(s): 三好大輔・狩俣寿枝・Z. -M. wang・甲元一也・杉本直己
  • Organizer: ICOMC2008
  • Place of Presentation: Rennes(フランス)
  • Year and Date: 2008-07-16
• [Presentation] Polymorphic nature of structure and stability of telomere DNAs depending on cellular surrounding factors (2008)
  • Author(s): D. Miyoshi
  • Organizer: 九州大学グローバルCOEプログラム第1回国際ワークショップ
  • Place of Presentation: 福岡
  • Year and Date: 2008-06-03
• [Presentation] Development of Functional Molecules Based on Nucleic Acids (2008)
  • Author(s): 三好大輔・中野修一・狩俣寿枝・井上真美子・中村かおり・岡裕人・Zhong-Ming Wnag・甲元一也・杉本直己
  • Organizer: 2008 Medical Chemistry and Developmental Therapeutics Symposium
  • Place of Presentation: Tucson(アメリカ)
  • Year and Date: 2008-04-17
• [Presentation] Non-canonical DNA structures for dynamic nanodevices (2008)
  • Author(s): D. Miyoshi
  • Organizer: FIBER International Symposium on Nucleic Acid Chemistry
  • Place of Presentation: 神戸
• [Presentation] Artificial DNA forming metal-responding nanowire, RSC Conference; Chemistry in the New World of Bioengineering and Synthetic Biology (2008)
  • Author(s): D. Miyoshi, H. T. Karimata, Z.-M. Wang, K. Koumoto, N. Sugimoto
  • Organizer: Oxford
  • Place of Presentation: 英国
• [Presentation] 核酸の構造安定性に及ぼす分子クラウディングとヒストン模倣ペプチドの影響 (2008)
  • Author(s): 三好大輔・中村かおり・Muhuri Sanjukta・狩俣寿枝・杉本直己
  • Organizer: 第3回バイオ関連化学合同シンポジウム
  • Place of Presentation: 神奈川
• [Presentation] DNA四重鎖の構造形成におけるカチオンと水分子の役割 (2008)
  • Author(s): 藤本健史・三好大輔・三村健太・狩俣寿枝・杉本直己
  • Organizer: 第3回バイオ関連化学合同シンポジウム
  • Place of Presentation: 神奈川
• [Presentation] 分子クラウディング環境下におけるテロメラーゼ活性の検討 (2008)
  • Author(s): Zhang Dong・Hao・Yu Hai-Qing・三好大輔・杉本直己
  • Organizer: 第3回バイオ関連化学合同シンポジウム
  • Place of Presentation: 神奈川
• [Presentation] Thermodynamics of DNA structures under molecular crowding conditions with neutral and positive charged cosolutes, Joint Symposium of the 18th International Roundtable on Nucleosides (2008)
  • Author(s): D. Miyoshi, K. Nakamura, S. Muhuri, H. T. Karimata, N. Sugimoto
  • Organizer: nucleotides and Nucleic Acids and the 35th International Symposium on Nucleic Acids Chemistry
  • Place of Presentation: 京都
• [Presentation] DNA nanostructures that respond to chemical stimuli (2008)
  • Author(s): 三好大輔・狩俣寿枝・Z.-M. Wang・甲元一也・杉本直己
  • Organizer: ICOMC2008
  • Place of Presentation: フランス
• [Presentation] 核酸の高次構造変化を利用した機能性ナノバイオマテリアルの開発 (2008)
  • Author(s): 三好大輔・Hai-Qing Yu・狩俣寿枝・杉本直己
  • Organizer: ナノバイオ若手ネットワーキングシンポジウム
  • Place of Presentation: 静岡
• [Presentation] Polymorphic nature of structure and stability of telomere DNAs depending on cellular surrounding factors (2008)
  • Author(s): 三好大輔
  • Organizer: 九州大学グローバルCOE プログラム第1回国際ワークショップ
  • Place of Presentation: 福岡
• [Presentation] 分子クラウディング状態における長鎖テロメアDNAの構造と安定性 (2008)
  • Author(s): 三好大輔・Hai-Qing Yu・Dong-Hao Zhang・杉本直己
  • Organizer: 日本ケミカルバイオロジー研究会第3回年会
  • Place of Presentation: 東京
• [Presentation] Development of Functional Molecules Based on Nucleic Acids (2008)
  • Author(s): D. Miyoshi・S. Nakano・H. Karimata・M. Inoue・K.Nakamura・H. Oka・Z.-M. Wang・ K.Koumoto・N. Sugimoto
  • Organizer: 2008 Medical Chemistry and Developmental Therapeutics Symposium, Tucson
  • Place of Presentation: 米国
• [Presentation] 化学的刺激に応答する機能性核酸マテリアルの開発 (2008)
  • Author(s): 三好大輔
  • Organizer: 日本化学会第88回春季年
  • Place of Presentation: 東京
• [Presentation] NanoBioNow(10)周辺の分子環境に応答するDNAナノスイッチの構築 (2008)
  • Author(s): 狩俣寿枝・三好大輔・藤本健史・甲元一也・Zhong-Ming, Wang・杉本直己
  • Organizer: 日本化学会第88回春季年会
  • Place of Presentation: 東京
• [Presentation] NanoBioNow (9) The structure of G-quadruplex for 5'-UTR mRNA of bcl-2 under molecular crowing condition (2008)
  • Author(s): 張東浩・三好大輔・杉本直己
  • Organizer: 日本化学会第88 回春季年会
  • Place of Presentation: 東京
• [Presentation] NanoBioNow(7)カチオン性ポルフィリンによるDNA四重鎖の構造制御 (2008)
  • Author(s): 村嶋貴之・三好大輔・松井淳・宮澤敏文・山田隆己・杉本直己
  • Organizer: 日本化学会第88回春季年会
  • Place of Presentation: 東京
• [Presentation] NanoBioNow(6)ポルフィリン修飾核酸を含むDNA二重鎖の構造と安定性 (2008)
  • Author(s): 佐伯裕美・村嶋貴之・松井淳・三好大輔・宮澤敏文・山田 隆己・杉本直己
  • Organizer: 日本化学会第88回春季年会
  • Place of Presentation: 東京
• [Presentation] NanoBioNow(5)DNA二重鎖による機能性DNA四重鎖の配向化 (2008)
  • Author(s): 三好大輔・K. Dutta・S. Muhuri・井上真美子・杉本直己
  • Organizer: 日本化学会第88回春季年会
  • Place of Presentation: 東京
• [Presentation] NanoBioNow(4)DNA四重鎖の熱力学的安定性に及ぼすカルテットとループの安定性の寄与 (2008)
  • Author(s): 藤本健史・狩俣寿枝・三好大輔・杉本直己
  • Organizer: 日本化学会第88回春季年会
  • Place of Presentation: 東京
• [Presentation] NanoBioNow(3)共存溶質の分子クラウディングによるフーグスティーン塩基対からなるDNA構造の安定化 (2008)
  • Author(s): 中村かおり・狩俣寿枝・三好大輔・大道達雄・杉本直己
  • Organizer: 日本化学会第88回春季年会
  • Place of Presentation: 東京
• [Presentation] NanoBioNow(6)ポルフィリン修飾核を含むDNA二重鎖の構造と安定性 (2008)
  • Author(s): 佐伯裕美・村嶋貴之・松井淳・三好大輔・宮澤敏文・山田 隆己・杉本直己
  • Organizer: 日本化学会第88回春季年会
  • Place of Presentation: 東京
• [Presentation] NanoBioNow(9)The structure of G-quadruplex for 5-UTR mRNA of bcl-2 under molecular crowing condition (2008)
  • Author(s): 張東浩・三好大輔・杉本直己
  • Organizer: 日本化学会第88回春季年会
  • Place of Presentation: 東京
• [Presentation] 化学的刺激に応答する機能性核酸マテリアルの開発 (2008)
  • Author(s): 三好大輔
  • Organizer: 日本化学会第88回春季年会
  • Place of Presentation: 東京
• [Presentation] Conformational switch of a functional nanowire based on the DNA G-quadruplex (2007)
  • Author(s): 狩俣寿枝・三好大輔・藤本健史・甲元一也・ Z.-M. Wang・杉本直己
  • Organizer: 5th International Symposium on Nucleic Acids Chemistry
  • Place of Presentation: 東京
• [Presentation] Effects of cosolutes on the thermodynamic stability of parallel DNA duplex and triplex (2007)
  • Author(s): 中村かおり・狩俣寿枝・大道達夫・三好大輔・杉本直己
  • Organizer: 5th International Symposium on Nucleic Acids Chemistry
  • Place of Presentation: 東京
• [Presentation] Development of functional nanobiodevices with DNAs (2007)
  • Author(s): D. Miyoshi・S. Nakano・H. Karimata・ M. Inoue・K. Nakamura・Y. Uotani・H. Oka・Z.-M. Wang・K. Koumoto・N. Sugimoto
  • Organizer: 2nd European Conference on Chemistry for Life Sciences
  • Place of Presentation: ポーランド
• [Presentation] 擬似細胞環境下における長鎖テロメア核酸の構造と熱力学的安定性 (2007)
  • Author(s): 三好大輔・Hai-Qing Yu・狩俣寿枝・杉本直
  • Organizer: 日本化学会生体関連化学部会シンポジウム
  • Place of Presentation: 仙台
• [Presentation] 化学入力に応答するユニバーサルDNA論理素子の開発 (2007)
  • Author(s): 三好大輔・井上真美子・杉本直己
  • Organizer: 日本化学会バイオテクノロジー部会シンポジウム
  • Place of Presentation: 仙台
• [Presentation] TELOMERE DNA-BASED LOGIC GATES (2007)
  • Author(s): 三好大輔・井上真美子・杉本直己
  • Organizer: The 2nd International Workshop on Approaches to Single-Cell Analysis
  • Place of Presentation: 東京
• [Presentation] Design and Development of Functional Molecules Based on Nucleic Acids, (2007)
  • Author(s): D. Miyoshi・S. Nakano・H. Karimata・ M. Inoue・K. Nakamura・Y. Uotani・ H. Oka・Z.-M. Wang・K. Koumoto・N. Sugimoto
  • Organizer: Gordon Research Conference (Nucleosides, Nucleotides & Oligonucleotides)
  • Place of Presentation: 米国
• [Presentation] 細胞内環境因子に応答するユニバーサルDNAロジックゲートの構築 (2007)
  • Author(s): 三好大輔・井上真美子・杉本直己
  • Organizer: 日本ケミカルバイオロジー研究会第2回年会
  • Place of Presentation: 東京
• [Presentation] Development of Functional DNAs Based on G-Quadruplexes (2007)
  • Author(s): D. Miyoshi・H.Q. Yu・H. Karimata・N. Sugimoto
  • Organizer: First International Meeting of Quadruplex DNA
  • Place of Presentation: 米国
• [Presentation] Development of Functional DNAs Based on G-Quadruplexes (2007)
  • Author(s): 三好大輔・H. Q. Yh・狩俣寿枝・杉本直己
  • Organizer: First International Meeting of Quadruplex DNA
  • Place of Presentation: Louisville, KY, USA
• [Presentation] 細胞内環境因子に応答するユニバーサルDMロジックゲートの構築 (2007)
  • Author(s): 三好大輔・井上真美子・杉本直己
  • Organizer: 日本ケミカルバイオロジー研究会 第2回年会
  • Place of Presentation: 京都
• [Presentation] Design and Development of Functional Molecules Based on Nucleic Acids (2007)
  • Author(s): 三好大輔・中野修一・狩俣寿枝・井上真美子・中村かおり・魚谷有希・岡裕人・Z.-M. Wang・甲元一也・杉本直己
  • Organizer: Gordon Research Conference (Nucleosides, Nucleotides & Oligonucleotides)
  • Place of Presentation: Newport, RI, USA
• [Presentation] 化学入力に応答するユニバーサルDNA論理素子の開発 (2007)
  • Author(s): 三好大輔・井上真美子・杉本直己
  • Organizer: 日本化学会バイオテクノロジー部会シンポジウム
  • Place of Presentation: 東京
• [Presentation] Effects of cosolutes on the thermodynamic stability of parallel DNA duplex and tripiex. (2007)
  • Author(s): 中村かおり・狩俣寿枝・大道達夫・三好大輔・杉本直己
  • Organizer: 2nd European Conference on Chemistry for Life Sciences
  • Place of Presentation: Wroclaw, Poland
• [Presentation] Development of functional nanobiodevices with DNAs (2007)
  • Author(s): 三好大輔・中野修一・狩俣寿枝・井上真美子・中村かおり・魚谷有希・岡裕人・Z.-M. Wang・甲元一也・杉本直己
  • Organizer: 5th International Symposium on Nucleic Acids Chemistry
  • Place of Presentation: 京都
• [Presentation] Conformational switch of a functional nanowire based on the DNA G-quadruplex (2007)
  • Author(s): 狩俣寿枝・三好大輔・藤本健史・甲元一也・Z.-M. Wang・杉本直己
  • Organizer: 5th International Symposium on Nucleic Acids Chemistry
  • Place of Presentation: 京都
• [Book] 核酸医薬の分子設計 (2009)
  • Author(s): 三好大輔・杉本直己
  • Publisher: 核酸医薬の最前線
• [Book] 超分子サイエンス&テクノロジー-基礎からイノベーションまで- (2009)
  • Author(s): 杉本直己、三好大輔
  • Publisher: 機能性核酸のテーラーメード設計
• [Book] 核酸医薬の最前線 (2009)
  • Author(s): 三好大輔、杉本直己
  • Publisher: 核酸医薬の分子設計
• [Book] 未来材料 (2007)
  • Author(s): 三好大輔・杉本直己
  • Total Pages: 9
  • Publisher: (株)エヌ・ティー・エス
• [Book] 化学 (2007)
  • Author(s): 杉本直己・三好大輔
  • Total Pages: 5
  • Publisher: 化学同人
• [Remarks]
  • URL: http://www.konan-first.jp/graduate/ti_204_a.html
• [Remarks] 学部・大学院ホームページ
  • URL: http://www.konan-first.jp
• [Remarks] 研究所ホームページ
  • URL: http://www.konan-fiber.jp
• [Remarks]
  • URL: http://fiber.konan-u.ac.jp/