| Budget Amount *help |
¥3,120,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥420,000)
Fiscal Year 2009: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2008: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2007: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Research Abstract |
Applicability of real-time PCR method was evaluated by cultivation experiments of Microcystis. After logarithmic growth phase, quantification results by both mcy A gene and 16S rRNA gene were similar to cell numbers measured by microscopic analysis. Increase in cell number and mcy A gene corresponded with increase in microcystin production. Simultaneous increase in mcy A gene and microcystin was observed in a field survey. These results proved that real-time PCR quantification can be applied to microcystin-producing blue-green algae, and it is promising to develop monitoring and growth inhibition technologies of toxic-producing blue-green algae.
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