Development of the novel process for obtaining large amount of embryonic bodies from embryonic stem cells using small microcapsules with hollow core

• Project/Area Number: 19760554
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Biofunction/Bioprocess
• Research Institution: Kyushu University
• Principal Investigator: SAKAI Shinji Kyushu University, 大学院・工学研究院, 助教 (20359938)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,630,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥330,000); Fiscal Year 2008: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2007: ¥2,200,000 (Direct Cost: ¥2,200,000)
• Keywords: マイクロカプセル / ES細胞 / 再生医療 / 細胞包括カプセル / アルギン酸 / セルロース / 胚様体 / 生体材料 / アイクロカプセル / バイオテクノロジー / 生物・生体工学

Research Abstract

ES細胞やiPS細胞を用いた再生医療の実現には、特定の臓器や組織の細胞に分化させる技術の他に、それらの細胞を大量に作り出す技術を開発することが必要である。本研究においては、そのための技術として、分化誘導を行う前段階の胚様体と呼ばれる球状の組織体を大量に調製するための培養容器の開発を行った。その結果、我々の身近に存在する海藻や植物からの抽出物を利用して直径約0.1mmの胚様体を大量に調製する容器を開発することに成功した。

Research Products

• [Journal Article] Feasibility of carboxymethylcellulose with phenol moieties as a material for mammalian cell-enclosing subsieve-size capsules (2008)
  • Author(s): Shinji Sakai, Syo Ito, Yuko Ogushi, Ichiro Hashimoto, Koei Kawakami
  • Journal Title: Cellulose vol.15 Pages: 723-729
  • Peer Reviewed
• [Journal Article] Agarose-gelatin conjugate membrane enhances proliferation of adherent cells enclosed in hollow-core microcapsules (2008)
  • Author(s): Shinji Sakai, Ichiro Hashimoto, Koei Kawakami
  • Journal Title: Journal of Biomaterials Science Polymer Edition vol.19 Pages: 937-944
  • Peer Reviewed
• [Journal Article] Production of cell-enclosing hollow-core agarose microcapsules via jetting in water-immiscible liquid paraffin and formation of embryoid body-like spherical tissues from mouse ES cells enclosed within these microcapsules (2008)
  • Author(s): Shinji Sakai, Ichiro Hashimoto, Koei Kawakami
  • Journal Title: Biotechnology and Bioengineering vol.99 Pages: 235-243
  • Peer Reviewed
• [Journal Article] Both Ionically and Enzymatically Crosslinkable Alginate-Tyramin e Conjugate as Materials for Cell Encapsulation (2008)
  • Author(s): Shinji SAKAI, et.al.
  • Journal Title: Journal of Biomedical Materials Resrch Prt A 85 Pages: 345-351
  • Peer Reviewed
• [Journal Article] Agarose-gelatin Conjugate Membrane Enhances Proliferation of Adherent Cells Enclosed in Hollow-core Microcapsules (2008)
  • Author(s): Shinji SAKAI, et.al.
  • Journal Title: Journal of Biomaterials Science, Polymer Edition 19 Pages: 937-944
  • Peer Reviewed
• [Journal Article] Feasibility of Carboxymethylcellulose with Phenol Moieties as a Material for Mammalian Cell-enclosing Subsieve-size Capsules (2008)
  • Author(s): Shinji SAKAI, et.al.
  • Journal Title: Cellulose 15 Pages: 723-729
  • Peer Reviewed
• [Journal Article] Production of Cell-enclosing Hollow-core Agarose Microcapsules via Jetting in Water-immiscible Liquid Paraffin and Formation of Embryoid body-like Spherical Tissues from Mouse ES Cells Enclosed within These Microcapsules (2008)
  • Author(s): Sakai, Shinji
  • Journal Title: Biotechnology and Bioengineering 99 Pages: 235-254
  • Peer Reviewed
• [Journal Article] Peroxidase-catalyzed cell-encapsulation in subsieve-size capsules of alginate with phenol moieties in water-immiscible fluid dissolving H2O2 (2007)
  • Author(s): Shinji Sakai, Ichiro Hashimoto, Yuko Ogushi, Koei Kawakami
  • Journal Title: Biomacromolecules vol.8 Pages: 2622-2626
  • Peer Reviewed
• [Presentation] Fabrication of cell-enclosing capsules with hollow-core via enzymatic functions (2008)
  • Author(s): Shinji Sakai, Syo Ito, Koei Kawakami
  • Organizer: XVI International Conference on Bioencapsulation
  • Place of Presentation: ダブリン(アイルランド)
  • Year and Date: 2008-09-05
• [Presentation] 微小中空カプセル作製法の開発とマウスES細胞からの胚様体様構造体の作製 (2008)
  • Author(s): 境 慎司
  • Organizer: 化学工学会2007年度年会
  • Place of Presentation: 静岡大学
  • Year and Date: 2008-03-17
• [Remarks] 化学工業日報2009年2月17日,一面記事「ES細胞から胚様体-多糖質カプセル用い大量培養-」として研究成果が紹介された。