Functional roles for retrotransposons in genome regulation

• Project/Area Number: 19770002
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Genetics/Genome dynamics
• Research Institution: National Institute of Genetics
• Principal Investigator: ICHIYANAGI Kenji National Institute of Genetics, 総合遺伝研究系, 助教 (70401560)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,780,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥480,000); Fiscal Year 2008: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2007: ¥1,700,000 (Direct Cost: ¥1,700,000)
• Keywords: ゲノム構築 / 機能 / 再編 / 発現 / 維持 / レトロトランスポゾン / SINE / DNA結合蛋白質 / ゲノム機能 / one-hybridスクリーニング / 転移

Research Abstract

MIRは哺乳類ゲノムに1万コピー以上存在するレトロトランスポゾンである。この配列はよく保存されており、何らかの機能を有していると考えられた。そこで、その機能をデコードするタンパク質を同定するため、酵母菌one-hybridスクリーニング系を用いて、この配列に結合するタンパク質をスクリーニングしたところ、ヒストンH2A.Zバリアントおよび機能未知のZnフィンガー・タンパク質を結合因子として同定した。

Research Products

• [Journal Article] Genetic evidence that non-homologous end joining repair pathway is involved in LINE retrotransposition (2009)
  • Author(s): Suzuki J., Yamaguchi K., Kajikawa M., Ichiyanagi K., Adachi N., Koyama H., Takeda S., and Okada N
  • Journal Title: PLoS Genet 5
  • Peer Reviewed
• [Journal Article] Genetic Evidence that the non-homologous end-joining repair pathway is involved in LINE retrotransnosition (2009)
  • Author(s): Suzuki, K
  • Journal Title: PLoS Genetics 5
  • Peer Reviewed
• [Journal Article] Mobility Pathways for vertebrate L1, L2, CR1, and RTE-clade retorotrans-posons (2008)
  • Author(s): Ichiyanagi K. and okada N
  • Journal Title: Mol. Biol. Evol 25 Pages: 1148-1157
  • Peer Reviewed
• [Journal Article] カモノハシ・ゲノムからみた哺乳類の進化 (2008)
  • Author(s): 一柳健司
  • Journal Title: 科学 78 Pages: 8817-8819
• [Journal Article] Mobility Pathways for Vertebrate Ll-, L2-, CR1-, and RTE-Clade Retrotransposons (2008)
  • Author(s): Ichiyanagi, K.
  • Journal Title: Molecular Biology and Evolution 25 Pages: 1148-1157
  • Peer Reviewed
• [Journal Article] Mobility Pathways for Vertebrate L1-, L2-, CR1-, and RTE-Clade Retrotransposons. (2008)
  • Author(s): Ichiyanagi, K.
  • Journal Title: Molecular Biology and Evolution (Online publication)
  • Peer Reviewed
• [Journal Article] Acquisition of Endonuclease Specificity during Evolution of L1 Retrotransposon (2007)
  • Author(s): Ichiyanagi, K.
  • Journal Title: Molecular Biology and Evolution 24 Pages: 2009-2015
  • Peer Reviewed
• [Journal Article] A new system for analyzing LINE retrotransposition in the chicken DT40 cell line widely used for reverse genetics (2007)
  • Author(s): Honda, H.
  • Journal Title: Gene 395 Pages: 116-124
  • Peer Reviewed
• [Presentation] Locus-specific hyper- and hypomethylation of mouse B1 elements in male germ cells (2009)
  • Author(s): Ichiyanagi K., Kitayama J., and Sasaki H
  • Organizer: in Genomic Impact of Eukaryotic Transposable Elements
  • Place of Presentation: Pacific Grove, CA, USA
• [Presentation] Locus-specific hyper- and hypo-methylation of mouse B1 elements in male germ cells (2009)
  • Author(s): Ichiyanagi, K
  • Organizer: Genomic Impact of Eukaryotic Transposable Elements
  • Place of Presentation: Pacific Grove, California, USA
• [Presentation] ゲノム配列を利用した脊椎動物レトロトランスポゾンの転移機構の解析 (2007)
  • Author(s): 一柳健司
  • Organizer: 第30回日本分子生物学会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-13