Why galactolipids are the major lipids in the photosynthetic membranes?

• Project/Area Number: 19770025
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: 植物生理・分子
• Research Institution: Saitama University
• Principal Investigator: AWAI Koichiro Saitama University, 大学院・理工学研究科, 助教 (80431732)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥240,000); Fiscal Year 2008: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2007: ¥2,600,000 (Direct Cost: ¥2,600,000)
• Keywords: チラコイド膜 / ガラクト脂質 / リン酸欠乏 / 光合成 / 糖転移酵素 / 葉緑体 / シアノバクテリア / 比較ゲノム解析

Research Abstract

酸素発生型光合成生物では、光合成反応の場であるチラコイド膜が、主にガラクト脂質で構成されていることが知られている。しかし、何故チラコイド膜に多量のガラクト脂質が存在するのかわかっていない。本研究では主要ガラクト脂質の1つ、ジガラクトシルジアシルグリセロール(DGDG)合成酵素遺伝子を、光合成研究のモデル生物であるラン藻で同定し、遺伝子破壊株を用いた研究から、DGDG がリン酸欠乏条件の光合成膜維持に必須であることを明らかにした。

Research Products

• [Journal Article] Type B monogalactosyldiacylglycerol synthases are involved in phosphate starvation-induced lipid remodeling and are crucial for low-phosphate adaptation. (2009)
  • Author(s): Kobayashi, K., Awai, K., Nakamura, M., Nagatani, A., Masuda, T. and Ohta, H.
  • Journal Title: Plant J. 57 Pages: 322-331
  • Peer Reviewed
• [Journal Article] Type B monogalactosyldiacylglycerol synthases are involved in phosphate starvati on-induced lipid remodeling and are crucial for low-phosphate adaptation (2009)
  • Author(s): Koichi Kobayashi
  • Journal Title: The Plant Journal 57 Pages: 322-331
  • Peer Reviewed
• [Journal Article] A small ATPase protein of Arabidopsis, TGD3, involved in chloroplast lipid import. (2007)
  • Author(s): Lu, B., Xu, C., Awai, K., Jones, A.D. and Benning C.
  • Journal Title: J. Biol. Chem. 282 Pages: 35945-35953
  • Peer Reviewed
• [Journal Article] Digalactosyldiacylglycerol is required for better photosynthetic growth of Synechocystis sp. PCC 6803 under phosphate limitation. (2007)
  • Author(s): Awai, K., Watanabe, H., Benning, C. and Nishida, I.
  • Journal Title: Plant Cell Physiol. 48 Pages: 1517-1523
  • NAID: 120006385866
  • Peer Reviewed
• [Journal Article] Digalactosyldiacylglycerol is required for better photosynthetic growth of Synechocystis sp. PCC6803 under phosphate limitation (2007)
  • Author(s): Koichiro Awai
  • Journal Title: Plant and Cell Physiology 48 Pages: 1517-1523
  • NAID: 120006385866
  • Peer Reviewed
• [Presentation] 糸状性シアノバクテリアにみられる糖脂質の構造解析 (2009)
  • Author(s): 渡辺英男、粟井光一郎、吉田充、西田生郎
  • Organizer: 日本植物生理学会年会
  • Place of Presentation: 愛知
  • Year and Date: 2009-03-21
• [Presentation] 糸状性シアノバクテリアにみられる糖脂質の構造解析 (2009)
  • Author(s): 渡辺英男
  • Organizer: 日本植物生理学会年会
  • Place of Presentation: 愛知(名古屋)
  • Year and Date: 2009-03-21
• [Presentation] ラン藻Synechocystis sp. PCC 6803 とSynechococcus sp. PCC 7942 の脂質要求性の違い (2009)
  • Author(s): 牧野渓史、粟井光一郎、小島幸治、西山佳孝、西田生郎
  • Organizer: 日本ゲノム微生物学会年会
  • Place of Presentation: 東京
  • Year and Date: 2009-03-06
• [Presentation] ラン藻Synechocystis sp.PCC6803とSynechococcus sp.PCC7942の脂質要求性の違い (2009)
  • Author(s): 牧野渓史
  • Organizer: 日本ゲノム微生物学会年会
  • Place of Presentation: 東京(後楽園)
  • Year and Date: 2009-03-06
• [Presentation] Biochemical analysis of a novel glycolipid from filamentous cyanobacteria. Gordon Research Conference, Plant Lipids: Structure (2009)
  • Author(s): Awai, K., Watanabe, H., Yoshida, M.. and Nishida, I.
  • Organizer: Metabolism & Function.
  • Place of Presentation: 米国
  • Year and Date: 2009-02-02
• [Presentation] Biochemical analysis of a novel glycolipid from filamentous cyanobacteria (2009)
  • Author(s): Koichiro Awai
  • Organizer: Gordon Research Conference, Plant Lipids : Structure, M etabolism & Function
  • Place of Presentation: 米国(ガルベストン)
  • Year and Date: 2009-02-02
• [Presentation] Molecular identification of genes forgalactolipid synthesis in cyanobacteria by comparative genomic analysis. (2008)
  • Author(s): Awai K., Ohta H., Benning C. and Nishida I.
  • Organizer: 18th International Symposium on Plant Lipids.
  • Place of Presentation: 仏国
  • Year and Date: 2008-07-21
• [Presentation] Molecular identification of genes for galactolipid synthesis in cyanobacteria by co mparative genomic analysis. (2008)
  • Author(s): Koichiro Awai
  • Organizer: 18^<th> International Symposium on Plant Lipids
  • Place of Presentation: 仏国(ボルドー)
  • Year and Date: 2008-07-21
• [Presentation] リン酸欠乏条件下におけるチラコイド膜糖脂質の役割 (2008)
  • Author(s): 渡辺英男, 粟井光一郎, Christoph Benning, 西田生郎
  • Organizer: 日本植物生理学会年会
  • Place of Presentation: 北海道
  • Year and Date: 2008-03-21
• [Presentation] リン酸欠乏条件下におけるチラコイド糖脂質の役割 (2008)
  • Author(s): 渡辺 英男
  • Organizer: 日本植物生理学会年会
  • Place of Presentation: 北海道(札幌)
  • Year and Date: 2008-03-21
• [Presentation] Synechocystis sp. PCC 6803 でDGDG はリン酸欠乏条件での生育に必須である (2007)
  • Author(s): 粟井光一郎
  • Organizer: ラン藻の分子生物学2007
  • Place of Presentation: 千葉
  • Year and Date: 2007-12-04
• [Presentation] Synechocystis sp. PCC6803でDGDGはリン酸欠乏条件での生育に必須である (2007)
  • Author(s): 粟井 光一郎
  • Organizer: ラン藻の分子生物学2007
  • Place of Presentation: 千葉(木更津)
  • Year and Date: 2007-12-04
• [Presentation] I. Digalactosyldiacylglycerol is required for better photosynthetic growth of Synechocystis sp. PCC 6803 under phosphate limitation. (2007)
  • Author(s): Awai, K., Watanabe, H., Benning, C. and Nishida,
  • Organizer: The 2nd Asian Symposium on Plant Lipids
  • Place of Presentation: 東京
  • Year and Date: 2007-12-02
• [Presentation] Digalactosyldiacylglycerol is required for better photosynthetic growth of Synechocystis sp. PCC6803 under phosphate limitation (2007)
  • Author(s): Koichiro Awai
  • Organizer: The 2nd Asian Symposium on Plant Lipids
  • Place of Presentation: 東京(渋谷)
  • Year and Date: 2007-12-02
• [Presentation] Identification of the glycosyltransferase involved in biosynthesis of the glycolipid specific to filamentous cyanobacteria. (2007)
  • Author(s): Awai, K. and Wolk, C.P.
  • Organizer: National Plant Lipid Cooperative.
  • Place of Presentation: 米国
  • Year and Date: 2007-06-08
• [Presentation] Identification of the glycosyltransferase involved in biosynthesis of the glycolipid specific to filamentous cyanobacteria (2007)
  • Author(s): Koichiro Awai
  • Organizer: National Plant Lipid Cooperative
  • Place of Presentation: 米国(Lake Tahoe)
  • Year and Date: 2007-06-08
• [Remarks]
  • URL: http://www.ipc.shizuoka.ac.jp/~dkawai/
• [Remarks]
  • URL: http://www.sci.saitama-u.ac.jp/molbiol/molbiol.htm