Regulatory mechanism of the activity of chloroplast ATP synthase by the ε subunit

• Project/Area Number: 19770027
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: 植物生理・分子
• Research Institution: Tokyo Institute of Technology
• Principal Investigator: KONNO Hiroki Tokyo Institute of Technology, 資源化学研究所, 助教 (80419267)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥3,740,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥540,000); Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2008: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2007: ¥1,400,000 (Direct Cost: ¥1,400,000)
• Keywords: 葉緑体 / ATP合成酵素 / 活性調節

Research Abstract

Physiological effects of C-terminal α-helices of the ε subunit and regulatory region of the γ subunit of chloroplast-type ATP synthase were investigated. We found that those regulatory mechanisms were important for maintenance of intracellular ATP levels in dark.

Research Products

• [Journal Article] impact of intrinsic ADP-Inhibition of Cyanocbacterial FoF1 conferred by the inherent inserted sequence into theγsubunit. (2010)
  • Author(s): Sunamura, E-I., Konno, H., Imashimizu-kobayashi, M., Sugano, Y., Hisabori, T. Physiological
  • Journal Title: Plant Cell Physiol. Vo51,No6(in press)
  • Peer Reviewed
• [Journal Article] Structural and functional analysis of the intrinsic inhibitor subunit epsilon of F1-ATPase from photosynthetic organisms. (2009)
  • Author(s): Yagi, H., Konno, H., Murakami-fuse, T., Isu, A., Oroguchi, T., Akutsu, H., Ikeguchi, M., Hisabori, T.
  • Journal Title: Biochem. J. 425(1) Pages: 85-94
  • Peer Reviewed
• [Journal Article] Molecular processes of inhibition and stimulation of ATP synthase caused by phytotoxin tentoxin. (2008)
  • Author(s): Meiss, E., Konno, H., Groth, G., Hisabori, T
  • Journal Title: J. Biol. Chem. 283(36) Pages: 24594-9
  • Peer Reviewed
• [Presentation] Inhibition of rotation of the cyanobacterial F1-ATPase by theεsubunit (2010)
  • Author(s): Hiroki Konno, Atsuko Isu, Tomoe Murakami-Fuse, Toru Hisabori
  • Organizer: International Symposium of Joint Research Net-work on Advanced Materials and Devices
  • Place of Presentation: 北海道ホテルニドム
• [Presentation] Inhibition of rotation of the cyanobacterial F_1-ATPase by the ε subunit (2010)
  • Author(s): 紺野宏記
  • Organizer: International Symposium of Joint Research Net-work on Advanced Materials and Devices
  • Place of Presentation: 北海道ホテルニドム
• [Presentation] 葉緑体型F1-ATPaseの回転におけるADP阻害とε阻害 (2009)
  • Author(s): 紺野宏記、井須敦子、村上-布施朋重、久堀徹
  • Organizer: 日本生体エネルギー研究会 第35回討論
  • Place of Presentation: 旭川医科大学
• [Presentation] Inhibition of rotation of the cyanobacterial F1-ATPase by theεsubunit (2009)
  • Author(s): Hiroki Konno, Atsuko Isu, Tomoe Murakami-Fuse
  • Organizer: International Symposium Innovative Nanoscience of Supermolecular Motor Proteins Working in Biomenbranes
  • Place of Presentation: 京都大学
• [Presentation] Inhibition of rotation of the cyanobacterial F_1-ATPase by the ε subunit (2009)
  • Author(s): 紺野宏記
  • Organizer: International Symposium Innovative Nanoscience of Supermolecular Motor Proteins Working in Biomenbranes
  • Place of Presentation: 京都大学
• [Presentation] 葉緑体型F1-ATPaseの回転におけるADP阻害とε阻害 (2009)
  • Author(s): 紺野宏記
  • Organizer: 日本生体エネルギー研究会 第35回討論会
  • Place of Presentation: 旭川医科大学
• [Presentation] Structure of the C-terminal domain of the epsilon subunit of chloroplast-type F1 (2008)
  • Author(s): Hiromasa Yagi, Hiroki Konno, Tomoe Murakami-Fuse, Hideo Akutu, Toru Hisabori
  • Organizer: BMB 2008
  • Place of Presentation: 神戸ポートアイランド
• [Presentation] ADP阻害とε阻害の関連性:一分子観察による検証 (2008)
  • Author(s): 紺野宏記、村上-布施朋重、久堀徹
  • Organizer: 日本生体エネルギー研究会第34回討論会
  • Place of Presentation: 東京医科歯科大学
• [Presentation] Structure of the C-terminal domain of theεsubunit of chloroplast-type F1 (2008)
  • Author(s): Hiromasa Yagi, Hiroki Konno, Tomoe Murakami-Fuse, Hideo Akutu, Toru Hisabori
  • Organizer: 15th European Bioenergetics Conference
  • Place of Presentation: Trinity College Dublin
• [Presentation] Structure of the C-terminal domain of the ε subunit of chloroplast-type F_1 (2008)
  • Author(s): 紺野宏記
  • Organizer: 15^<th> European Bioenergetics Conference
  • Place of Presentation: Trinity College Dublin
• [Presentation] ADP阻害とε阻害の関連性 : 一分子観察による検証 (2008)
  • Author(s): 紺野宏記
  • Organizer: 日本生体エネルギー研究会第34回討論会
  • Place of Presentation: 東京医科歯科大学
• [Presentation] Structure of the C-terminal domain of the epsilon subunit of chloroplast-type F_1 (2008)
  • Author(s): 紺野宏記
  • Organizer: BMB 2008
  • Place of Presentation: 神戸ポートアイランド
• [Presentation] Thermosynechococcus elongatus BP-1のF1-ATPaseにおけるγ-εサブユニット間の相互作用 (2007)
  • Author(s): 紺野宏記、村上-布施朋重、久堀徹
  • Organizer: 日本生体エネルギー研究会第33回討論会
  • Place of Presentation: 山口大学
• [Presentation] Regulation of Cyanobacterial F1-ATPase rotation (2007)
  • Author(s): Hiroki Konno, Tomoe Murakami-Fuse, Fumihiko Fujii, Fumie Koyama, Hanayo Ueoka-Nakanishi, Chan-Gi Pack, Masataka Kinjo, Toru Hisabori
  • Organizer: Gordon Research Conference (Molecular & Cellular Bioenergetics)
  • Place of Presentation: Proctor Academy
• [Presentation] Regulation of Cyanobacterial F_1-ATPase rotation (2007)
  • Author(s): 紺野 宏記
  • Organizer: Gordon Research Conference (Molecular & Cellular Bioenergetics)
  • Place of Presentation: Proctor Academy
• [Presentation] Thermosynechococcus elongates BP-1のF_1-ATPaseにおけるγ-εサブユニット間の相互作用 (2007)
  • Author(s): 紺野 宏記
  • Organizer: 日本生体エネルギー研究会 第33回討論会
  • Place of Presentation: 山口大学吉田キャンパス
• [Book] 光合成研究法, 北海道大学低温科学研究所・日本光合成研究会共編 (2009)
  • Author(s): 紺野宏記、久堀徹