Blue-light regulation of gene expression in purple bacteria

• Project/Area Number: 19770028
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: 植物生理・分子
• Research Institution: Tokyo Institute of Technology
• Principal Investigator: MASUDA Shinji Tokyo Institute of Technology, バイオ研究基盤支援総合センター, 准教授 (30373369)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,790,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥390,000); Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2007: ¥2,100,000 (Direct Cost: ¥2,100,000)
• Keywords: 環境応答 / 光受容体 / 光合成細菌 / 遺伝子発現

Research Abstract

1)光受容体AppAがリン酸化することを明らかにした。
2)リン酸化部位がT30であることを明らかにした。
3)リン酸化は二成分制御系のレスポンスレギュレーターとの機能的相互作用に重要であることを明らかにした。

Research Products

• [Journal Article] Crucial role in light signal transduction for the conserved Met93 of the BLUF protein PixD/Slr1694 (2008)
  • Author(s): Masuda, S., Hasegawa, K., Ohta, H. and Ono, T.
  • Journal Title: Plant Cell Physiol. 49 Pages: 1600-1606
  • NAID: 10027334810
  • Peer Reviewed
• [Journal Article] Regulation of aerobic photosystem synthesis in the purple bacterium Rhodospirillum centenum (2008)
  • Author(s): Masuda, S., Berleman, J., Hasselbring, B. M. and Bauer, C. E.
  • Journal Title: Photochem. Photobiol. Sci. 7 Pages: 1267-1272
  • Peer Reviewed
• [Journal Article] Possible targets of "magic spots" in plant signaling (2008)
  • Author(s): Masuda, S., Tozawa, Y. and Ohta, H.
  • Journal Title: Plant Signaling Behavior 3 Pages: 1021-1023
  • Peer Reviewed
• [Journal Article] Expression profiling of four RelA/SpoT-like proteins, homologues of bacterial stringent factors, in Arabidopsis thaliana (2008)
  • Author(s): Mizusawa, K., Masuda, S. and Ohta, H.
  • Journal Title: Planta 228 Pages: 553-562
  • Peer Reviewed
• [Journal Article] The bacterial stringent response, conserved in chloroplasts, controls plant fertilization (2008)
  • Author(s): Masuda, S., Mizusawa, K., Narisawa, T., Tozawa, Y., Ohta, H. and Takamiya, K.
  • Journal Title: Plant Cell Physiol. 49 Pages: 135-141
  • NAID: 10027328528
  • Peer Reviewed
• [Journal Article] Expression profiling of four RelA/SpoT-like proteins, homologues of bacte rial stringent factors, in Arabidopsis thaliana (2008)
  • Author(s): Mizusawa, K., Masuda, S. and Ohta, H.
  • Journal Title: Planta 228 Pages: 553-562
  • Peer Reviewed
• [Journal Article] Regulation of aerobic photosystem synthesis in the purple bacterium Rhodosnirillurn centenurn (2008)
  • Author(s): Masuda, S., Berleman, J., Hasselbring, B. M. and Bauer, C. E.
  • Journal Title: Photochem. Photobiol. Sci. 7 Pages: 1267-1272
  • Peer Reviewed
• [Journal Article] Crucial role in light signal transduction for the conserved Met93 of the BLUF protein PixD/Slr1694 (2008)
  • Author(s): Masuda, S., Hasegawa, K., Ohta, H. and Ono, T.
  • Journal Title: Plant Cell Physiol 49 Pages: 1600-1606
  • NAID: 10027334810
  • Peer Reviewed
• [Journal Article] The bacterial stringent response,conserved in chloroplasts,controls plant fertilization (2008)
  • Author(s): Masuda, S., Mizusawa, K., Narisawa, T., Tozawa, Y., Ohta, H., Takamiya, K.
  • Journal Title: Plant Cell Physiol. 49 Pages: 135-141
  • NAID: 10027328528
  • Peer Reviewed
• [Journal Article] The significance of Type II and PrxQ peroxiredoxins for antioxidative stress response in the purple bacterium Rhodobacter sphaeroides (2007)
  • Author(s): Wakita, M., Masuda, S., Motohashi, K., Hisabori, T., Ohta, H. and Takamiya, K.
  • Journal Title: J. Biol. Chem. 282 Pages: 27792-27801
  • Peer Reviewed
• [Journal Article] Transient dimerization and conformational change of a BLUF protein: YcgF (2007)
  • Author(s): Nakasone, Y., Ono, T., Ishii, A., Masuda, S. and Terazima, M.
  • Journal Title: J. Am. Chem. Soc 129 Pages: 7028-7035
  • Peer Reviewed
• [Journal Article] The critical role of a hydrogen bond between Gln63 and Trp104 in the blue-light sensing BLUF domain that controls AppA activity (2007)
  • Author(s): Masuda, S., Tomida, Y., Ohta, H. and Takamiya, K.
  • Journal Title: J. Mol. Biol. 368 Pages: 1223-1230
  • Peer Reviewed
• [Journal Article] Purification and reconstitution of PYP-phytochrome with biliverdin and 4-hydroxycinnamic acid (2007)
  • Author(s): Chung, Y., Masuda, S. and Bauer, C. E.
  • Journal Title: Methods Enzymol. 422 Pages: 184-189
  • Peer Reviewed
• [Journal Article] The significance of Type IIand PrxQ Peroxiredoxins for antioxidative stress response in the purple bacterium Rhodobacter sphaeroides (2007)
  • Author(s): Wakita, M., Masuda, S., Motohashi, K., Hisabori, T., Ohta, H., Takamiya, K.
  • Journal Title: J.Biol.Chem. 282 Pages: 27792-27801
  • Peer Reviewed
• [Journal Article] Purification and reconstitution of PYP-phytochrome with biliverdin and 4-hydroxycinnamic acid. (2007)
  • Author(s): Chung, Y., Masuda, S., Bauer, C.E.
  • Journal Title: Methods Enzymol. 422 Pages: 184-189
  • Peer Reviewed
• [Journal Article] Transient dimerization and conformational change of a BLUF protein:YcgF (2007)
  • Author(s): Nakasone, Y., Ono, T., Ishii, A., Masuda, S., Terazima, M.
  • Journal Title: J.Am.Chem.Soc. 129 Pages: 7028-7035
  • Peer Reviewed
• [Presentation] 新規青色光受容体の同定と機能の解析 (2009)
  • Author(s): 増田真二
  • Organizer: 日本植物生理学会年会
  • Place of Presentation: 名古屋大学
  • Year and Date: 2009-03-24
• [Presentation] 新規青色光受容体の同定と機能の解析 (2009)
  • Author(s): 増田真二
  • Organizer: 日本植物生理学会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-03-22
• [Presentation] 紅色細菌の青色光受容BLUFタンパク質AppAのリン酸化を介した光シグナル伝達 (2008)
  • Author(s): 増田真二、富田祥之、松岡大介、太田啓之、高宮建一郎
  • Organizer: 日本植物学会
  • Place of Presentation: 高知
  • Year and Date: 2008-09-25
• [Presentation] 紅色細菌の青色光受容BLUFタンパク質AppAのリン酸化を介した光シグナル伝達 (2008)
  • Author(s): 増田真二、富田祥之、松岡大介、徳富哲、太田啓之、高宮建一郎
  • Organizer: 日本植物学会年会
  • Place of Presentation: 高知大学
  • Year and Date: 2008-09-20
• [Presentation] BLUFタンパク質における青色光シグナルの受容と伝達の分子機構 (2008)
  • Author(s): 増田真二
  • Organizer: 大阪大学蛋白質研究所セミナー「生物における光情報変換の一般性と多様性」
  • Place of Presentation: 大阪
  • Year and Date: 2008-04-15
• [Presentation] BLUFタンパク質における青色光シグナルの受容と伝達の分子機構 (2008)
  • Author(s): 増田真二
  • Organizer: 大阪大学蛋白質研究所セミナー
  • Place of Presentation: 大阪大学
  • Year and Date: 2008-04-10
• [Presentation] BLUFタンパク質AppAはリン酸化を介した光シグナル伝達を行う (2008)
  • Author(s): 富田祥之、増田真二、富田祥之、松岡大介、太田啓之、高宮建一郎
  • Organizer: 日本植物生理学会
  • Place of Presentation: 札幌
  • Year and Date: 2008-03-20
• [Presentation] 青色光受容体AppAはリン酸化を介した光シグナル伝達を行う (2008)
  • Author(s): Tomida, Y., Masuda, S., Matsuoka, D., MIkutomi, S., Ohta, H., Takamiya, K.
  • Organizer: 第49回日本植物生理学会年会
  • Place of Presentation: 札幌コンベンションセンター
  • Year and Date: 2008-03-20
• [Presentation] Site-directed mutagenesis study of BLUF proteins (2008)
  • Author(s): Masuda, S
  • Organizer: Gordon Research Conferences, Photosensory Receptors & Signal Transduction
  • Place of Presentation: Ventura, USA
  • Year and Date: 2008-01-30
• [Presentation] Physiological analysis of Q63L and W1O4A mutant BLUF protein,AppA,in Rhodobacter sphaeroides (2007)
  • Author(s): Masuda, S., Tomida, Y., Ohta, H., Takamiya, K.
  • Organizer: 第45回日本生物物理学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2007-12-22
• [Presentation] 紅色細菌の光合成装置形成における青色光受容BLUFタンパク質AppAのQ63L/W104A変異の影響 (2007)
  • Author(s): 増田真二、富田祥之、太田啓之、高宮建一郎
  • Organizer: 日本生物物理学会第43回年会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-20
• [Presentation] Complementation analysis of an Arabidopsis PORA knockdown mutant by cyanobacterial PORs (2007)
  • Author(s): Masuda, S., Ikeda, R., Masuda, T., Hashimoto, H., Tsuchiya, T., Mimuro, M., Ohta, H. and Takamiya, K.
  • Organizer: 7th International Conference on Tetrapyrrole Photoreceptors in Photosynthetic Organisms
  • Place of Presentation: Kyoto, Japan
  • Year and Date: 2007-12-10
• [Presentation] Blue-light control of bacterial photosystem synthesis (2007)
  • Author(s): Masuda, S.
  • Organizer: International Symposium on Biosynthesis of Tetrapyrroles
  • Place of Presentation: Shiga, Japan
  • Year and Date: 2007-12-08
• [Presentation] Cyanobacterial NADPH:protochlorophyllide oxidoreductase (POR) compensates for knockdown mutation of PORA in Arabidopsis thaliana (2007)
  • Author(s): Masuda, S., Ikeda, R., Masuda, T., Tsuchiya, T., Mimuro, M., Ohta, H. and Takamiya, K.
  • Organizer: 14th International Congress of Photosynthesis
  • Place of Presentation: Glasgow, Scotland
  • Year and Date: 2007-08-15
• [Book] Regulation of genes by light. In The Purple Photosynthetic Bacteria: Advances in Photosynthesis and Respiration, Vol. 28(Hunter, C.N., Daldal, F., Thurnauer, M.C., Beatty, J.T., Eds.) (2008)
  • Author(s): Klug, G. and Masuda, S.
  • Publisher: Springer, Dordrecht, The Netherlands
• [Book] Regulation of genes by light. In The Purple Photosynthetic Bacteria : Advances in Photosynthesis and Respiration (Hunter, C.N., Daldal, F., Thurnauer, M.C., Beatty, J.T., Eds.) (2008)
  • Author(s): Klug, G. and Masuda, S.
  • Publisher: Springer, Dordrecht, The Netherlands