Analysis of the CP12L function as a new Calvin Cycle regulatory factor, functioning in C4 plants

• Project/Area Number: 19770034
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: 植物生理・分子
• Research Institution: Hiroshima University
• Principal Investigator: FURUMOTO Tsuyoshi Hiroshima University, 大学院・理学研究科, 准教授 (30313208)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,690,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥390,000); Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2007: ¥2,000,000 (Direct Cost: ¥2,000,000)
• Keywords: 環境応答 / 代謝制御 / 酵素活性調節 / C4光合成 / 複合体形成 / カルビンベンソン回路 / 酸化還元 / NADP

Research Abstract

C4 植物特有の明期における光律速光合成特性に必要な新しいカルビン回路制御に、新規に単離したCP12Lを介したNADP依存的なGAPDH・PRK複合体形成が関与している可能性が考えられ、その検証を行った。In vitro 複合体再構成に成功し、これらの3つの因子が複合体形成をなしえることを証明し、さらに暗処理した葉の抽出液中においても同様の複合体の存在を認めた。これはin vivo においてもこれらの複合体が行われておることを強く示唆するデータである。

Research Products

• [Journal Article] Maize C4-form phosphoenolpyruvate carboxylase engineered to be functional in C3 plants: mutations for diminished sensitivity to feedback inhibitors and for increased substrate affinity (2008)
  • Author(s): Endo T, Mihara Y, Furumoto T, MatsumuraH, Kai Y, Izui K.
  • Journal Title: Journal Exp Bot. 59 Pages: 1811-1818
  • Peer Reviewed
• [Journal Article] Light response of C4 photosynthesis in Amaranthus cruentus: The relationship between CO2 leakiness and in vivo activities of C4 photosyntheticenzymes (2008)
  • Author(s): Tazoe Y, Hanba Y-T, Furumoto T, Izui K, Noguchi K, Terashima I.
  • Journal Title: Plant Cell Physiol 49 Pages: 19-29
  • Peer Reviewed
• [Journal Article] Maize C4-form phosphoenolpyruvate carboxylase engineered to be functional in C3 plants : mutations for diminished sensitivity to feedback inhibitors and for increased substrate affinity (2008)
  • Author(s): Endo T
  • Journal Title: Journal Experimental Boanyt 59 Pages: 1811-1818
  • Peer Reviewed
• [Journal Article] Light response of C4 photosynthesis in Amaranthus cruentus : The relationship between CO2 leakiness and in vivo activities of C4 photosynthetic enzymes (2008)
  • Author(s): Tazoe Y
  • Journal Title: Plant and Cell Physiology 49 Pages: 19-29
  • Peer Reviewed
• [Journal Article] Light response of C4 photosynthesis in Amaranthus cruentus: The relationship between C02 leakiness, in vivo activities of C4 photosynthetic enzymes (2008)
  • Author(s): Tazoe Y.
  • Journal Title: Plant, Cell Physiology 49 Pages: 19-29
  • Peer Reviewed
• [Journal Article] Phosphorylation of phosphoenolpyruvate carboxylase is not essential for high photosynthetic rates in the C4 species Flaveria bidentis (2007)
  • Author(s): Furumoto T, Izui K, Quinn V, Furbank RT, von Caemmerer S.
  • Journal Title: Plant Physiol 144 Pages: 1936-1945
  • Peer Reviewed
• [Journal Article] Phosphorylation of phosphoenolpyruvate carboxylase is not essential for high photosynthetic rates in the C4 species Flaveria bidentis (2007)
  • Author(s): Furumoto T.
  • Journal Title: Plant Physiology 144 Pages: 1936-1945
  • Peer Reviewed
• [Journal Article] AGFl, an ATHook protein, is necessary for the negative feedback of At GA3ox1 encoding GA 3-oxidase (2007)
  • Author(s): Matsushita A.
  • Journal Title: Plant Physiology 143 Pages: 1152-1162
  • Peer Reviewed
• [Presentation] "Identification of aplastid pyruvate transporter" International Symposium "he Ins and Outs of Chloroplasts" (2008)
  • Author(s): Furumoto T, Yamaguchi T, Ichie Y, Takahashi Y, Izui K.
  • Organizer: Icho-Kaikan
  • Place of Presentation: Osaka University
• [Presentation] Identification of a plastid Na+ dependent pyruvate transporter in plants (2007)
  • Author(s): Furumoto T.
  • Organizer: C4 and CAM satelite meeting of the 14th International Congress of photosynthsis
  • Place of Presentation: Univ. of Cambridge, Cambridge,
  • Year and Date: 2007-07-20
• [Presentation] Identification of aplastid Na+ dependent pyruvate transporter in plants (2007)
  • Author(s): Furumoto T, Yamaguchi T, Ichie Y, Takahashi Y, Izui K.
  • Organizer: C4 and CAM satelite meeting of the 14th International Congress of photosynthesis
  • Place of Presentation: Cambridge, UK
• [Patent(Industrial Property Rights)] 葉緑体ピルビン酸輸送体タンパク質よびそれをコードする遺伝子 (2007)
  • Inventor(s): 古本強
  • Industrial Property Rights Holder: 古本強
  • Filing Date: 2007-07-19