Analysys of the phototropin signaling system in Physcomitrella patens

• Project/Area Number: 19770038
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: 植物生理・分子
• Research Institution: Ritsumeikan University
• Principal Investigator: KASAHARA Masahiro Ritsumeikan University, 生命科学部, 准教授 (70361748)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,730,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥330,000); Fiscal Year 2008: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2007: ¥2,300,000 (Direct Cost: ¥2,300,000)
• Keywords: フォトトロピン / 光応答 / 光受容体 / ヒメツリガネゴケ / シグナル伝達

Research Abstract

植物の光受容体であるフォトトロピンの光情報受容とシグナル伝達機構の解明を目指し、ヒメツリガネゴケをモデル系として研究を行った。これまでに、ヒメツリガネゴケのフォトトロピンを大腸菌、またはヒメツリガネゴケで発現させる系を構築した。今後、リン酸化活性の測定系、タンパク質精製系を改良することによって、フォトトロピンシグナル系の解析に有用な研究基盤になると考えられる。

Research Products

• [Journal Article] DNA molecule manipulation by motor proteins for itsanalysis in single molecule level.Anal. (2008)
  • Author(s): Yokokawa R., Miwa J., Tarhan MC., Fujita H., and Kasahara M.
  • Journal Title: Bioanal. Chem. 391 Pages: 2735-2743
  • Peer Reviewed
• [Journal Article] Chloroplast outer envelope protein CHUP1 is essential for chloroplast anchorage to the plasma membrane and chloroplast movement. (2008)
  • Author(s): Oikawa K., Yamasato A., Kong SG., Kasahara M., Nakai M., Takahashi F., Ogura Y., Kagawa T., and Wada M.
  • Journal Title: Plant Physiol 148 Pages: 829-842
  • Peer Reviewed
• [Journal Article] Chloroplast outer envelope protein CHUP1 is essential for chloroplast anchorage to the plasma membrane and chloroplast movement (2008)
  • Author(s): Oikawa K., Yamasato A., Kong SG., Kasahara M., Nakai M., Takahashi F., Ogura Y., Kagawa T., and Wada M.
  • Journal Title: Plant Physiol 148 Pages: 829-842
  • Peer Reviewed
• [Journal Article] DNA molecule manipulation by motor proteins for its analysis in single molecule level (2008)
  • Author(s): Yokokawa R., Miwa J., Tarhan MC., Fujita H., and Kasahara M.
  • Journal Title: Anal. Bioanal. Chem 391 Pages: 2735-2743
  • Peer Reviewed
• [Journal Article] DNA molecule manipulation by motor proteins for its analysis in single molecule level. (2008)
  • Author(s): Yokokawa R., Miwa J., Tarhan MC., Fujita H., and Kasahara M.
  • Journal Title: Anal. Bioanal. Chem. (In press)
  • Peer Reviewed
• [Journal Article] a newly found novel photoreceptor required for photomorphogenesis in stramenopiles. (2007)
  • Author(s): Takahashi F., Yamagata D., Ishikawa M., Fukamatsu Y., Ogura Y., Kasahara M., Kiyosue T., Kikuyama M., Wada M., and Kataoka H. AUREOCHROME:
  • Journal Title: Proc. Natl. Acad. Sci. U. S. A. 104 Pages: 19625-19630
  • Peer Reviewed
• [Journal Article] The TIR-NBS but not LRRdomains of two novel N-like proteinsare functionally competent to inducethe elicitor p50-dependenthypersensitive response (2007)
  • Author(s): Gao JS., Sasaki N., Kanegae H., Konagaya K., Takizawa K., Hayashi N., Okano Y., Kasahara M., Matsushita Y., and Nyunoya H.
  • Journal Title: Physiol. Mol.Plant Pathol. 71 Pages: 78-87
  • Peer Reviewed
• [Journal Article] AUREOCHROME: a newly found novel photoreceptor required for photomorphogenesis in stramenopiles. (2007)
  • Author(s): Takahashi F., Yamagata D., Ishikawa M., Fukamatsu Y., Ogura Y., Kasahara M., Kiyosue T., Kikuyama M., Wada M., and Kataoka H.
  • Journal Title: Proc. Natl. Acad. Sci. U.S.A. 104 Pages: 19625-19630
  • Peer Reviewed
• [Journal Article] The TIR-NBS but not LRR domains of two novel N-like proteins are functionally competent to induce the elicitor p50-dependent hypersensitive response. (2007)
  • Author(s): Gao JS., Sasaki N., Kanegae H., Konagaya K., Takizawa K., Hayashi N., Okano Y., Kasahara M., Matsushita Y., and Nyunoya H.
  • Journal Title: Physiol. Mol. Plant Pathol. 71 Pages: 78-87
  • Peer Reviewed
• [Presentation] ヒメツリガネゴケにおけるブラシノステロイドの働き (2009)
  • Author(s): 津森康一郎、丹生谷博、笠原賢洋
  • Organizer: 日本植物生理学会年会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-03-22
• [Presentation] ヒメツリガネゴケにおけるブラシノステロイドの生理作用 (2009)
  • Author(s): 津森康一郎、丹生谷博、笠原賢洋
  • Organizer: 第50回日本植物生理学会年会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-03-22
• [Presentation] Light green phenotype of a phototropin triple disruptant of Physcomitrella patens (2007)
  • Author(s): T.Takemoto and M.Kasahara
  • Organizer: International Symposium on Biosynthesis of Tetrapyrroles
  • Place of Presentation: 草津
  • Year and Date: 2007-12-08
• [Presentation] Phototropin and LOV proteins (2007)
  • Author(s): M.Kasahara
  • Organizer: International Symposium on Biosynthesis of Tetrapyrroles
  • Place of Presentation: 草津
  • Year and Date: 2007-12-08
• [Remarks]
  • URL: http://www.ritsumei.ac.jp/lifescience/skbiot/kasahara/LAB.html