Analysis of HSP90-Regulated Signaling Mechanisms in Plant

• Project/Area Number: 19770040
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: 植物生理・分子
• Research Institution: National Institute for Basic Biology
• Principal Investigator: YAMADA Kenji National Institute for Basic Biology, 高次細胞機構研究部門, 助教 (00360339)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,880,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥480,000); Fiscal Year 2008: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2007: ¥1,800,000 (Direct Cost: ¥1,800,000)
• Keywords: シロイヌナズナ / HSP90 / 熱ショック応答 / シャペロン / 熱ショックタンパク質 / 転写制御 / 高温耐性 / HSF

Research Abstract

シロイヌナズナを用い,HSP90がどのような細胞内因子と結合し,その働きを調節するかを調べるため,HSP90阻害剤を処理したシロイヌナズナの応答を解析した.その結果,植物においてHSP90は熱ショック応答を制御していることと,オーキシン応答を制御していることがわかった.HSP90はシロイヌナズナにおいて常に細胞内に蓄積している熱ショック転写制御因子,HSFA1と結合し,この結合はHSP90阻害剤により阻害された.HSP90阻害剤処理によりHSFA1が活性化された.これらのことから,HSP90は熱ショック転写制御因子と結合しその活性を負に制御していることが示唆された.一方,オーキシンで誘導されるレポーター遺伝子をもつシロイヌナズナを用い,HSP90阻害剤で,オーキシン応答が顕著に抑制されることがわかった.さらに,HSP90阻害剤で,内在性のオーキシン応答遺伝子の発現が顕著に抑制された.これらのことから,HSP90はオーキシン応答を制御することが示唆された.

Research Products

• [Journal Article] Constitutive and inducible ER bodies of Arabidopsis thaliana accumulate distinct β-glucosidases (2009)
  • Author(s): K. Ogasawara, et.al.
  • Journal Title: Plant Cell Physiol. 50(in press)
  • Peer Reviewed
• [Journal Article] NAI2 is an endoplasmic reticulum body component that enables ER body formation in Arabidopsis thaliana (2008)
  • Author(s): K. Yamada, A. J. Nagano, M. Nishina, I. Hara-Nishimura, M. Nishimura
  • Journal Title: Plant Cell 20 Pages: 2529-2540
  • Peer Reviewed
• [Journal Article] Cytosolic heat shock protein 90 regulates heat shock transcription factor in Arabidopsis thaliana (2008)
  • Author(s): K. Yamada, M. Nishmura
  • Journal Title: Plant Signal. Behay 3 Pages: 660-662
• [Journal Article] Cytosolic heat shock protein 90 regulates heat shock transcription factor in Arabidopsis thaliana (2008)
  • Author(s): K. Yamada and M. Nishimura
  • Journal Title: Plant Signal. Behav. 3 Pages: 660-662
• [Journal Article] NAI2 is an endoplasmic reticulum body component that enables ER body formation in Arabidopsis thaliana (2008)
  • Author(s): K. Yamada, et.al.
  • Journal Title: Plant Cell 20 Pages: 2529-2540
  • Peer Reviewed
• [Journal Article] Cytosolic heat shock protein 90 regulates heat shock transcription factor in Arabidopsis thaliana. (2008)
  • Author(s): K. Yamada and M. Nishimura
  • Journal Title: Plant Signal. Behav. 3(In press)
• [Journal Article] Cytosolic HSP90 regulates the heat shock response that is responsible for heat acclimation in Arabidopsis thaliana (2007)
  • Author(s): K. Yamada, Y. Fukao, M. Hayashi, M. Fukazawa, I. Suzuki, M. Nishimura
  • Journal Title: Jr. Biol. Chem. 282(52) Pages: 37794-37804
  • Peer Reviewed
• [Journal Article] Cytosolic HSP90 regulates the heat shock response that is responsible for heat acclimation in Arabidopsis thaliana. (2007)
  • Author(s): K. Yamada, et. al.
  • Journal Title: J. Biol. Chem. 282 Pages: 37794-37804
  • Peer Reviewed
• [Presentation] NAI2 is an endoplasmic reticulum (ER) body component that enables ER body formation (2008)
  • Author(s): Yamada, A. J. Nagano, M. Nishina, I. Hara-Nishimura, M. Nishimura
  • Organizer: The 55th NIBB Conference Arabidopsis Workshop 2008
  • Place of Presentation: Aichi, Japan
• [Presentation] NAI2 is an endoplasmic reticulum (ER) body component that enables ER body formation (2008)
  • Author(s): K. Yamada
  • Organizer: The 55^<th> NIBB Conference on Arabidopsis Workshop
  • Place of Presentation: Aichi, Japan
• [Presentation] シロイヌナズナの薬剤耐性獲得におけるHSP90の役割 (2007)
  • Author(s): 山田健志,西村幹夫
  • Organizer: 第30回日本分子生物学会,第80回日本生化学会大会合同大会
  • Place of Presentation: 横浜
• [Presentation] HSP90 regulates heat shock response that is responsible for heat adaptation (2007)
  • Author(s): K. Yamada, Y. Fukao, M. Hayashi, M. Fukazawa, I. Suzuki, M. Nishimura
  • Organizer: 18th International Conference on Arabidopsis Research
  • Place of Presentation: Beijing, China
• [Presentation] 熱処理によるHSP90活性の減少が熱応答を引き起こす (2007)
  • Author(s): 山田健志,深沢美津江,鈴木育,西村幹夫
  • Organizer: 第48回日本植物生理学会年会
  • Place of Presentation: 松山
• [Presentation] HSP90 regulates heat shock response that is responsible for heat adaptation. (2007)
  • Author(s): K. Yamada
  • Organizer: 18th International Conference on Arabidopsis Research
  • Place of Presentation: Beijing, China
• [Remarks]
  • URL: http://www.nibb.ac.jp/celmech/jp/
• [Remarks] ホームページ
  • URL: http://www.nibb.ac.jp/sections/nishimura.html
• [Remarks]
  • URL: http://www.nibb.ac.jp/sections/nishimura.html