Identification and characterization of small RNA specifically acting in Arabidopsis endosperm

• Project/Area Number: 19770043
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: 植物生理・分子
• Research Institution: National Institute for Basic Biology (2008); The Institute of Physical and Chemical Research (2007)
• Principal Investigator: TATEMATSU Kiyoshi National Institute for Basic Biology, 植物器官形成学研究室, 助教 (00373324)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,220,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥420,000); Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2007: ¥1,400,000 (Direct Cost: ¥1,400,000)
• Keywords: 種子発芽 / 小分子RNA / 胚乳特異的 / 次世代シーケンス技術 / AP2転写因子 / miR172 / AtABA2 / エクスパンシン遺伝子 / small RNA / 胚乳軟弱化 / ABA生合成

Research Abstract

シロイヌナズナ種子吸水時に胚乳で遺伝子発現制御に関与する小分子RNAの同定を行った。胚乳で特異的に発現する小分子RNAを次世代シーケンス技術により塩基配列決定を行った。その結果、胚乳特異的に発現するAP2転写因子群を認識する既知のmiR172や、アブシジン酸の生合成酵素AtABA2や細胞壁成分の加水分解に関わるエクスパンシン遺伝子をターゲットとする新奇小分子RNA候補が得られた。こうした遺伝子群が胚乳で機能することで種子発芽が制御されていると予想された。

Research Products

• [Journal Article] CHOTTO1, a double AP2 domain protein of Arabidopsis thaliana, regulates germination and seedling growth under excess supply of glucose and nitrate (2009)
  • Author(s): Yamagishi, K. *, Tatematsu, K.*, Yano, R., Preston, J., Kitamura, S., Takahashi, H., McCourt, P., Kamiya, Y., and Nambara, E
  • Journal Title: Plant Cell Physiol 50 Pages: 330-340
  • NAID: 10027337592
  • Peer Reviewed
• [Journal Article] The Arabidopsis abscisic acid catabolic gene CYP707A2 plays a key role in nitrate control of seed dormancy (2009)
  • Author(s): Matakiadis, T., Alboresi, A., Jikumaru, Y., Tatematsu, K., Pichon, O., Renou, J.-P., Kamiya, Y., Nambara, E., and Truong, H.-N.
  • Journal Title: Plant Physiol 149 Pages: 949-960
  • Peer Reviewed
• [Journal Article] The Arabidopsis abscisic acid catabolic gene CYP707A2 plays a key role in nitrate control of seed dormancy (2009)
  • Author(s): Matakiadis, et.al.
  • Journal Title: Plant Physiology 149 Pages: 949-960
  • Peer Reviewed
• [Journal Article] CHOTTO1, a double AP2 domain protein of Arabidopsis thaliana, regulates germination and seedling growth under excess supply of glucose and nitrate (2009)
  • Author(s): Yamagishi and Tatematsu, et.al.
  • Journal Title: Plant and Cell Physiology 50 Pages: 330-340
  • NAID: 10027337592
  • Peer Reviewed
• [Journal Article] Phytochrome-and gibberellin-mediated regulation of abscisic acid metabolism during germination of photoblastic lettuce seeds (2008)
  • Author(s): Sawada, Y., Aoki, M., Nakaminami, K., Mitsuhashi, W., Tatematsu, K., Kushiro, T., Koshiba, T., Kamiya, Y., Inoue, Y., Nambara, E., and Toyomasu, T.
  • Journal Title: Plant Physiol 146 Pages: 1386-1396
  • Peer Reviewed
• [Journal Article] Co-regulation of ribosomal protein genes as an indicator of growth status : Comparative transcriptome analysis on axillary shoots and seeds in Arabidopsis (2008)
  • Author(s): Tatematsu, K., Kamiya, Y., and Nambara, E.
  • Journal Title: Plant Signal. Behav 3 Pages: 450-452
• [Journal Article] Transcription factor AtTCP14 regulates embryonic growth potential during seed germination in Arabidopsis thaliana (2008)
  • Author(s): Tatematsu, K., Nakabayashi, K., Kamiya, Y., and Nambara, E
  • Journal Title: Plant J. 53 Pages: 42-52
  • Peer Reviewed
• [Journal Article] Phytochrome and gibberellin-mediated regulation of abscisic acid metabolism during germination of photoblastic lettuce seeds (2008)
  • Author(s): Sawada, et.al.
  • Journal Title: Plant Physiology 146 Pages: 1386-1396
  • Peer Reviewed
• [Journal Article] Transcription factor AtTCP14 regulates embryonic growth potential during seed germination in Arabidopsis thaliana (2008)
  • Author(s): Kiyoshi Tatematsu
  • Journal Title: The Plant Journal 53 Pages: 45-52
  • Peer Reviewed
• [Journal Article] 発芽過程のホルモン調節-胚の生長ポテンシャルと胚乳の軟弱化の調節- (2007)
  • Author(s): 立松圭、南原英司
  • Journal Title: 植物の生長調節 Vol.42 No.2 Pages: 139-145
  • NAID: 110006533779
• [Journal Article] 発芽過程のホルモン調節-胚の生長ポテンシャルと胚乳の軟弱化の調節 (2007)
  • Author(s): 立松 圭
  • Journal Title: 植物の生長調節 42 Pages: 139-145
  • NAID: 110006533779
• [Presentation] Small RNA expression profiling during seed germination in Arabidopsis thaliana (2008)
  • Author(s): Tatematsu, K., Matsui, A., Morosawa, T., Kaminuma, E., Okamoto, M., Toyoda, T., Shinozaki, K., Kamiya, Y., Seki, M., and Nambara, E
  • Organizer: The 55th NIBB Conference Arabidopsis Workshop 2008
  • Place of Presentation: Okazaki
• [Presentation] Comparative studies on non-dormant Col and dormant Cvi seeds: Common and polymorphic regulations on molecular mechanism of seed imbibition in Arabidopsis (2008)
  • Author(s): Kimura, M., Preston, J., Tatematsu, K., Kanno, Y., Toh, S., Kawakami, N., Kamiya, Y., and Nambara, E.
  • Organizer: 19th Intl. Conf
  • Place of Presentation: Arabidopsis Res., Montreal
• [Presentation] Functional genomics on seed germination in Arabidopsis (2008)
  • Author(s): Nambara, E., Tatematsu, K., and Kamiya, Y.
  • Organizer: 6th Canadian Plant Genomics Workshop
  • Place of Presentation: Toronto
• [Presentation] シロイヌナズナ種子吸水時の遺伝子発現解析 (2008)
  • Author(s): 立松圭、Jeremy Preston、菅野裕理、藤茂雄、川上直人、神谷勇治、南原英司
  • Organizer: 第49回日本植物生理学会年会
  • Place of Presentation: 札幌
• [Presentation] Functional genomics on seed germination in Arabidopsis (2008)
  • Author(s): Nambara, et.al.
  • Organizer: 6th Canadian Plant Genomics Workshop
  • Place of Presentation: Toronto, Canada
• [Presentation] Comparative studies on non-dormant Col and dormant Cvi seeds : Common and polymorphic regulations on molecular mechanism of seed imbibition in Arabidopsis (2008)
  • Author(s): Kimura, et.al.
  • Organizer: 19th Intl. Conf. Arabidopsis Res.
  • Place of Presentation: Montreal, Canada
• [Presentation] Small RNA expression profiling during seed germination in Arabidopsis thaliana (2008)
  • Author(s): Tatematsu, et.al.
  • Organizer: The 55th NIBB Conference Arabidopsis Workshop 2008
  • Place of Presentation: Okazaki, Aichi, Japan
• [Presentation] Regulation of endogenous levels of abscisic acid in photoblastic lettuce seeds (2007)
  • Author(s): Sawada, Y., Aoki, M., Nakaminami, K., Mitsuhashi, W., Tatematsu, K., Jikumaru, Y., Kushiro, T., Kamiya, Y., Inoue, Y., Nambara, E., and Toyomasu, T
  • Organizer: 19th Intl. Conf. Plant Growth Substances Association Meeting
  • Place of Presentation: Puerto Vallarta
• [Presentation] Studies on early events in response to seed imbibition in Arabidopsis (2007)
  • Author(s): Preston, J., Tatematsu, K., Kamiya, Y., and Nambara, E
  • Organizer: 2nd ISSS Workshop on Molecular Aspects of Seed Dormancy and Germination
  • Place of Presentation: Salamanca
• [Presentation] Studies on early events in response to seed imbibition in Arabidopsis (2007)
  • Author(s): Preston Jeremy
  • Organizer: 2nd ISSS Workshop on Molecular Aspects of Seed Dormancy and Germination
  • Place of Presentation: スペイン・サラマンカ
• [Book] 第15章発芽関連遺伝子の解析発芽生物学種子発芽の生理・生体・分子機構,種生物学会編 (2009)
  • Author(s): 南原英司、立松圭、内藤哲
  • Publisher: 文一総合出版
• [Book] 発芽生物学種子発芽の生理・生体・分子機構, 種生物学会編 (2009)
  • Author(s): 南原ら
  • Total Pages: 465
  • Publisher: 文一総合出版