Analysis of molecular mechanisms of endoreduplication through chloroplast-dependent regulation.

• Project/Area Number: 19770044
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: 植物生理・分子
• Research Institution: The Institute of Physical and Chemical Research
• Principal Investigator: YOSHIZUMI Takeshi The Institute of Physical and Chemical Research, 植物ゲノム機能研究チーム, 研究員 (80342872)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥3,840,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥540,000); Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2008: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2007: ¥1,500,000 (Direct Cost: ¥1,500,000)
• Keywords: 発生・分化 / エンドリデュプリケーション / 葉緑体 / 情報伝達 / 細胞サイズ / 細胞周期 / エンドリデュブリケーション

Research Abstract

We focused on the molecular mechanisms of endoreduplication controlled by chloroplast. Because two genes relating chloroplast were identified as the corresponding genes of dominant mutants, ilp2-D and ilp5-D that shows increased polyploidy. To examine physiological function of these genes, we analyzed loss of these genes mutants. However, these mutants did not showed significant phenotypes on polyploidy levels.
We newly developed a unique construction of RNAi for the knock-down of these genes, and published a paper describing several advantage points of this method.

Research Products

• [Journal Article] Simple construction of plant RNAi vectors using long oligonucleotides. (2009)
  • Author(s): Higuchi M, Yoshizumi T, Kuriyama T, Hara H, Akagi C, Shimada H, Matsui M
  • Journal Title: J. Plant Res. 122 Pages: 477-482
  • NAID: 10025180875
• [Journal Article] Arabidopsis MBF1s control leaf cell cycle and its expansion. (2009)
  • Author(s): Tojo T, Tsuda K, Yoshizumi T, Ikeda A, Yamaguchi J, Matsui M, Yamazaki KI.
  • Journal Title: Plant Cell Physiol. 50 Pages: 254-264
  • NAID: 10027337233
• [Journal Article] Simple construction of plant RNAi vectors using long oligonucleotides (2009)
  • Author(s): Higuchi M et al.
  • Journal Title: Journal of Plant Research 122 Pages: 477-482
  • Peer Reviewed
• [Journal Article] Arabidopsis MBF1s control leaf cell cycle and its expansion (2009)
  • Author(s): Tojo, et al.
  • Journal Title: Plant Cell Physiology 50 Pages: 254-264
  • Peer Reviewed
• [Journal Article] Quantitative analysis of heterogeneous spatial distribution of Arabidopsis leaf trichomes using micro X-ray computed tomography. (2008)
  • Author(s): Kaminuma E, Yoshizumi T, Wada T, Matsui M, Toyoda T.
  • Journal Title: Plant J. 56 Pages: 470-482
• [Journal Article] RETARDED GROWTH OF EMBRYO1, a New Basic Helix-Loop-Helix Protein, Expresses in Endosperm to Control Embryo Growth. (2008)
  • Author(s): Kondou Y, Nakazawa M, Kawashima M, Ichikawa T, Yoshizumi T, Suzuki K, Ishikawa A, Koshi T, Matsui R, Muto S, Matsui M.
  • Journal Title: Plant Physiol. 147 Pages: 1924-1935
• [Journal Article] The DNA replication checkpoint aids survival of plants deficient in the novel replisome factor ETG1. (2008)
  • Author(s): Takahashi N, Lammens T, Boudolf V, Maes S, Yoshizumi T, De Jaeger G, Witters E, Inze D, De Veylder L.
  • Journal Title: EMBO J. 27 Pages: 1840-1851
• [Journal Article] The DNA replication checkpoint aids survival of plants deficient in the novel replisome factor ETG1 (2008)
  • Author(s): Takahashi, et al.
  • Journal Title: EMBO Journal 27 Pages: 1840-1851
  • Peer Reviewed
• [Journal Article] Quantitative analysis of heterogeneous spatial distribution of Arabidopsis leaf trichomes using micro X-ray computed tomography (2008)
  • Author(s): Kaminuma, et al.
  • Journal Title: Plant Journal 56 Pages: 470-482
  • Peer Reviewed
• [Presentation] SD5, a homologue of spliceosome subunit, regulates proliferation in post-seedling development (2009)
  • Author(s): Takeshi Yoshizumi et al.
  • Organizer: 21th International conference on Arabidopsis Research
  • Place of Presentation: エジンバラ、スコットランド
  • Year and Date: 2009-07-01
• [Presentation] SD5, a homologue of spliceosome subunit, is essential for proliferation in post-seedling development. (2009)
  • Author(s): Yoshizumi T, Hongo H, Kuromori T, Horii Y, Imura Y, Kamiya A, Shimada H, Matsui M.
  • Organizer: 20th International Conference on Arabidopsis Research
  • Place of Presentation: United Kingdom.
• [Presentation] スプライセオソームの構成因子と相同性を持つシロイヌナズナSD5の欠損は、細胞増殖の抑制を引き起こす (2009)
  • Author(s): 吉積毅, 本郷洋明, 黒森崇, 井村優子, 神谷麻子, 島田浩章, 松井南
  • Organizer: 第50回日本植物生理学会年会
  • Place of Presentation: 名古屋
• [Presentation] 機能獲得型変異株を用いたエンドリデュプリケーション制御機構解明の試み (2008)
  • Author(s): 吉積毅、本郷洋明、原博子、島田浩章、松井南
  • Organizer: 植物生理学会第49回日本植物生理学会年会
  • Place of Presentation: 札幌コンベンションセンター
  • Year and Date: 2008-03-20
• [Presentation] 新規ploidy変異株increased level of polyploidy4-D(ilp-D)の解析 (2008)
  • Author(s): 原博子、吉積毅、島田浩章、松井南
  • Organizer: 植物生理学会第49回日本植物生理学会年会
  • Place of Presentation: 札幌コンベンションセンター
  • Year and Date: 2008-03-20
• [Presentation] スプライオソームの構成因子と相同性を持つSD5の欠損は、シロイヌナズナの芽生えで致死性を示す (2008)
  • Author(s): 本郷洋明、吉積毅、黒森崇、神谷麻子、島田浩章、松井南
  • Organizer: 植物生理学会第49回日本植物生理学会年会
  • Place of Presentation: 札幌コンベンションセンター
  • Year and Date: 2008-03-20
• [Presentation] SD5, a homologue of spliceosome subunit, is essential for proliferation in post-seedling development. (2008)
  • Author(s): Yoshizumi T, Hongo H, Kuromori T, Horii Y, Imura Y, Kamiya A, Shimada H, Matsui M.
  • Organizer: 19th International Conference on Arabidopsis Research
  • Place of Presentation: Canada
• [Presentation] スプライセオソームの構成因子と相同性を持つシロイヌナズナSD5の欠損は、細胞増殖の抑制を引き起こす (2008)
  • Author(s): 吉積毅, 本郷洋明, 黒森崇, 井村優子, 神谷麻子, 島田浩章, 松井南
  • Organizer: 第31回日本分子生物学会年会
  • Place of Presentation: 神戸
• [Book] Plant Cell Monographs (Chapter name: Plant cell growth signalling and its link to ploidy.) (2008)
  • Author(s): Yoshizumi T, Breuer C, Matsui M, Sugimoto-Shirasu K.
  • Publisher: Springer
• [Book] Plant cell growth signalling and its link to ploidy.(eds. Beemster G and Bogre L, The Plant Growth Regulators) (2007)
  • Author(s): Yoshizumi T, Breuer C, Minami M, Sugimoto-Shirasu K
  • Publisher: Springer
• [Remarks]
  • URL: http://pfgweb.gsc.riken.jp/indexJ.html
• [Remarks]
  • URL: http://pfgweb.gsc.riken.jp/publicationsJ.html