forward genetic approach toward identifying gene(s) suppresses dwarf and lethal phenotypes of Arabidopsis mekk1 mutant

• Project/Area Number: 19770045
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: 植物生理・分子
• Research Institution: The Institute of Physical and Chemical Research
• Principal Investigator: ICHIMURA Kazuya The Institute of Physical and Chemical Research, 植物免疫研究チーム, 研究員 (70321726)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,780,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥480,000); Fiscal Year 2008: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2007: ¥1,700,000 (Direct Cost: ¥1,700,000)
• Keywords: 細胞死 / 防御反応 / プロテインキナーゼ / MAPキナーゼカスケード / リン酸化 / 抵抗性遺伝子 / 病害抵抗性 / MAPキナーゼ経路 / 植物免疫 / 細胞内情報伝達

Research Abstract

PAMP情報伝達に関わるシロイヌナズナMEKK1遺伝子の欠損変異体は、構成的な防御反応により、強い矮性を伴う致死形質を示す。この表現型の原因遺伝子を解明するため、1)MEKK1調節ドメインの発現誘導により、欠損変異体表現型の再現系を構築した。2)上記の形質転換シロイヌナズナを用いたEMSスクリーニングにより、矮性致死表現型を抑制する変異体を単離した。また、ポジショナルクローニングに向けた条件検討を行った。

Research Products

• [Journal Article] Negative regulation of PAMP-triggered immunity by an E3 ubiquitin ligase triplet in Arabidopsis. (2008)
  • Author(s): Trujillo,M., Ichimura, K., Casais, C. and Shirasu, K.
  • Journal Title: Current Biology 18 Pages: 1396-1401
  • Peer Reviewed
• [Journal Article] Negative Regulation of PAMP-Triggered Immunity by an E3 Ubiquitin Ligase Triplet in Arabidopsis (2008)
  • Author(s): Marco, Trujillo
  • Journal Title: Current Biology 18 Pages: 1396-1401
  • Peer Reviewed
• [Journal Article] CERK1, a LysM receptor kinase, is essential for chitin elicitor signaling in Arabidopsis. (2007)
  • Author(s): Ichimura, K. (10名中5番目)
  • Journal Title: Proc. Acad. Natl. Sci. U.S.A 109 Pages: 19613-19618
  • Peer Reviewed
• [Journal Article] The Mitogen-Activated Protein Kinase Cascade MKK3-MPK6 Is an Important Part of the Jasmonate Signal Transduction Pathway in Arabidopsis. (2007)
  • Author(s): Ichimura, K. (8名中3番目)
  • Journal Title: Plant Cell 19 Pages: 805-818
  • Peer Reviewed
• [Journal Article] CERK1, a LysM receptor kinase, is essential for chitin elicitor signaling in Arabidopsis. (2007)
  • Author(s): Miya, A., et. al.
  • Journal Title: Proc. Acad. Natl. Sci. U.S.A. 109 Pages: 19613-19618
  • Peer Reviewed
• [Presentation] シロイヌナズナMEKK1に結合するU-box型ユビキチンリガーゼの解析 (2009)
  • Author(s): 市村和也
  • Organizer: 日本植物生理学会 第50回年会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-03-22
• [Presentation] シロイヌナズナMEKK1に結合するU-box型ユビキチンリガーゼの解析 (2008)
  • Author(s): 市村和也
  • Organizer: 植物生理学会第50回年会
  • Place of Presentation: 名古屋
  • Year and Date: 2008-03-21
• [Presentation] シロイヌナズナmekk1変異体表現形を抑制する変異体の単離 (2008)
  • Author(s): 市村和也
  • Organizer: 植物生理学会第49回年会
  • Place of Presentation: 札幌
  • Year and Date: 2008-03-20
• [Presentation] シロイヌナズナmekk1変異体表現形を抑制する変異体の単離 (2008)
  • Author(s): 市村和也
  • Organizer: 第49回日本植物生理学会年会
  • Place of Presentation: 札幌
• [Book] Signal Cross Talk in Plant Stress Responses (2009)
  • Author(s): Fuminori Takahashi
  • Publisher: Wiley-Blackwell(印刷中)
• [Book] Plant Mitogen Activated Protein Kinase cascades in Signaling Crosstalk. Chapter 2, In Signal Crosstalk in Plant Stress Responses. (Yoshioka, K. Shinozaki, K. eds)
  • Author(s): Takahashi, F., Ichimura, K., Shinozaki, K., Shirasu, K.
  • Publisher: Ames, Iowa, Blackwell publishing(In press)
• [Remarks]
  • URL: http://www.riken.jp/r-world/info/release/press/2007/071120/index.html
• [Remarks]
  • URL: http://www.riken.jp/r-world/info/release/press/2008/080905_2/index.html
• [Remarks]
  • URL: http://www.riken.jp/r-world/info/release/press/2008/080905_2/index.html
• [Remarks]
  • URL: http://www.riken.jp/r-world/info/release/press/2007/071120/index.html