Cell biological analysis of vacuole formation machinery in plant cells

• Project/Area Number: 19770052
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Morphology/Structure
• Research Institution: The Institute of Physical and Chemical Research
• Principal Investigator: TOYOOKA Kiminori The Institute of Physical and Chemical Research, 機能開発研究グループ, 研究員 (10360596)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,790,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥390,000); Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2007: ¥2,100,000 (Direct Cost: ¥2,100,000)
• Keywords: 液胞 / 電子顕微鏡 / 膜タンパク質 / 高圧凍結技法 / シロイヌナズナ / 根 / タバコ

Research Abstract

高圧凍結技法と免疫電顕法を組み合わせ、液胞の由来やその形成機構を明らかにすることを目的とした。液胞膜タンパク質PPaseは分化している細胞内で、ゴルジ体由来の小胞、その小胞のクラスター、リング状の構造体、液胞膜に局在することが明らかとなり、分化の終わった細胞ではゴルジ体と液胞膜のみに存在することがわかった。さらに、PPase変異体、オートファジー機能欠損株を用いた電顕解析により、PPaseが存在するリング構造は形態形成に必須あることを明らかにした。

Research Products

• [Journal Article] Multidrug and toxic compound extrusion-typm transporters implicated in vacuolar sequestratyon of nicotine in tobacco roots. (2009)
  • Author(s): Shoji, T., Inai, K., Yazaki, Y., Sato,Y., Takase, H.,Shitan, N., Yazaki, K., Goto, Y., Toyooka, K., Matsuoka, K. and Hashimoto, T.
  • Journal Title: Plant Physiol. 149 Pages: 708-718
  • Peer Reviewed
• [Journal Article] A Chloroplastic UDP-glucose pyrophosphorylase from Arabidopsis : A committing enzyme for the first step of sulfolipid biosynthesis. (2009)
  • Author(s): Okazaki, Y., Shimojima, M., Sawada, Y., Toyooka, K., Narisawa, T., Mochida, K., Tanaka, H., Matsuda, F., Hirai, A., Yokota Hirai, M., Ohta, H., and Saito, K.
  • Journal Title: Plant Cell. (in press)
  • Peer Reviewed
• [Journal Article] Identification and characterization of the NA+/H+ antiporter NHAS3 from the thylakoid membrane of synechocystis SP. PCC 6803. (2009)
  • Author(s): Tsunekawa K, Shijuku T, Hayashimoto M, Kojima Y, Onai K, Morishita M, Ishiura M, Kuroda T, Nakamura T, Kobayashi H, Sato M, Toyooka K, Matsuoka K, Omata T, Uozumi N.
  • Journal Title: J Biol Chem. (in press)
  • Peer Reviewed
• [Journal Article] A mobile secretory vesicle cluster involved in mass transport from the Golgi to plant cell exterior. (2009)
  • Author(s): Toyooka, K., Goto, Y., Asatsuma, S., Koizumi, M., Mitsui, T. and Matsuoka, K.
  • Journal Title: Plant Cell. (in press)
  • Peer Reviewed
• [Journal Article] The roles of actin cytoskeleton and microtubules for membrane recycling of a food vacuole in Tetrahymena thermophila. (2009)
  • Author(s): Sugita M, Nakano K, Sato M, Toyooka K, Numata O.
  • Journal Title: Cell Motil Cytoskeleton. (in press)
  • Peer Reviewed
• [Journal Article] Identifying new components participating in the secondary cell wall formation of vessel elements in Zinnia and Arabidopsis. (2009)
  • Author(s): Endo, S., Pesquet, E., Yamaguchi, M., Tashiro, G., Sato, M., Toyooka, K., Nishikubo, N., Utagawa-Motose, M., Kubo, M., Fukuda, H. and Demura, T.
  • Journal Title: Plant Cell. (in press)
  • Peer Reviewed
• [Journal Article] EFC/F-BAR proteins and the N-WASP-WIP complex induce membrane curvature-dependent actin polymerization. (2008)
  • Author(s): Takano K, Toyooka K and Suetsugu S
  • Journal Title: EMBO J. 27 Pages: 2817-2828
  • Peer Reviewed
• [Journal Article] EFC/F-BAR proteins and the N-WASP-WIP complex inducemembrane-curvature dependent actin polymerization (2008)
  • Author(s): Takano, et al
  • Journal Title: EMBO J. 27 Pages: 2817-2828
  • Peer Reviewed
• [Journal Article] Cytological and Biochemical Analysis of COF1, an Arabidopsis Mutant of an ABC Transporter Gene. (2007)
  • Author(s): Ukitsu, H., Kuromori, T., Toyooka K., Goto, Y., Matsuoka, K., Sakuradani, E., Shimizu, S., Kamiya, A., Imura, Y., Yuguchi, M., Wada, T., Hirayama, T., Shinozaki, K.
  • Journal Title: Plant Cell Physiol. 48 Pages: 1524-1533
  • NAID: 10027005334
  • Peer Reviewed
• [Journal Article] Development of the series of gateway binary vectors, pGWB, realize efficient construction of fusion genes for plant transformation. (2007)
  • Author(s): Nakagawa, T., Kurose, T., Hino, T., Tanaka, K., Kawamukai, M., Niwa, Y., Toyooka, K., Matsuoka, K., Jinbo, T.and Kimura, T
  • Journal Title: J. Biosci. Bioeng. 104 Pages: 34-41
  • Peer Reviewed
• [Journal Article] Novel regulation of MHC class II function in B cells. (2007)
  • Author(s): Matsuki Y, Ohmura-Hoshino M, Goto E, Aoki M, Mito-Yoshida M, Uematsu M, Hasegawa, T, Koseki H, Ohara O, Nakayama M, Toyooka K, Matsuoka K, Hotta H, Yamamoto A and Ishido S.
  • Journal Title: EMBO J. 26 Pages: 846-854
  • Peer Reviewed
• [Journal Article] Novel regulation of MHC class II function in B cells (2007)
  • Author(s): Matsuki. Y
  • Journal Title: EMBO Journal 26 Pages: 846-854
  • Peer Reviewed
• [Journal Article] Development of the series of gateway binary vectors, pGWB, realize efficient construction of fusion genes for plant transformation. (2007)
  • Author(s): Nakagawa, T.
  • Journal Title: J. Biosci. Bioeng. 104 Pages: 34-41
  • Peer Reviewed
• [Journal Article] Cytological and Biochemical Analysis of COF1, an Arabidopsis Mutant of an ABC Transporter Gene. (2007)
  • Author(s): Ukitsu, H.
  • Journal Title: Plant Cell Physiol. 48 Pages: 1524-1533
  • Peer Reviewed
• [Journal Article] MATE-type Transporters Implicated in Vacuolar Sequestration of Nicotine in Tobacco Roots
  • Author(s): Shoji, et al
  • Journal Title: Plant Physiol. (In press)
  • Peer Reviewed
• [Journal Article] ペプチドグリカン層をもつ葉緑体であるシアネレの分裂とFtsZリング形成
  • Author(s): 佐藤繭子, 他
  • Journal Title: 顕微鏡(日本顕微鏡学会編) (印刷中)
  • Peer Reviewed
• [Presentation] 高圧凍結技法による液胞形成過程の免疫組織化学的解析 (2009)
  • Author(s): 豊岡公徳、後藤友美、佐藤繭子、黒森崇、吉本光希、前島正義、松岡健
  • Organizer: 第50回日本植物生理学会年会
  • Place of Presentation: 名古屋
• [Presentation] 高圧凍結技法による植物液胞形成過程の微細構造解析 (2009)
  • Author(s): 後藤友美、佐藤繭子、若崎眞由美、河合たか子、松岡健、豊岡公徳
  • Organizer: 第65回日本顕微鏡学会
  • Place of Presentation: 仙台
• [Presentation] Secretory vesicle clusterを介した分泌機構の解析 (2008)
  • Author(s): 豊岡公徳、後藤友美、佐藤繭子、松岡健
  • Organizer: 第49回日本植物生理学会年会
  • Place of Presentation: 札幌
• [Presentation] 植物組織凍結超薄ライブラリーの作製とその免疫電子顕微鏡観察法の検討 (2008)
  • Author(s): 佐藤繭子、後藤友美、松岡健、篠崎一雄、豊岡公徳
  • Organizer: 第49回日本植物生理学会年会
  • Place of Presentation: 札幌
• [Presentation] 高圧凍結技法による植物試料の微細構造観察法および免疫電顕法の検討 (2008)
  • Author(s): 豊岡公徳、後藤友美、佐藤繭子、河合たか子
  • Organizer: 第24回医学生物電子顕微鏡技術学会学術講演会
  • Place of Presentation: 逗子
• [Remarks]
  • URL: http://labs.psc.riken.jp/gdrg/Japanese/Publications_j.html
• [Remarks]
  • URL: http://labs.psc.riken.jp/gdrg/Japanese/Publications_j.html
• [Remarks]
  • URL: http://labs.psc.riken.jp/gdrg/Japanese/Publications_j.html