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The study of D-arginine met bolism and substrate specificity of arginine kinase from Sbellastrte indica

Research Project

Project/Area Number 19770058
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Animal physiology/Animal behavior
Research InstitutionKochi University

Principal Investigator

UDA Kouji  Kochi University, 教育研究部・自然科学系, 助教 (10448392)

Project Period (FY) 2007 – 2008
Project Status Completed (Fiscal Year 2008)
Budget Amount *help
¥3,700,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥300,000)
Fiscal Year 2008: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2007: ¥2,400,000 (Direct Cost: ¥2,400,000)
Keywords代謝生理 / 比較生化学 / D-アミノ酸 / アルギニンキナーゼ / クレアチンキナーゼ
Research Abstract

環形動物類のケヤリの生体内にはD-及びL-アルギニンを共に基質として利用し,D-及びL-アルギニンリン酸を合成することのできる特異なアルギニンキナーゼが存在している。
本研究では,このケヤリ・アルギニンキナーゼのアミノ酸置換変異体を多数作製し,その酵素活性の変化を検討することで,触媒機能に関与するアミノ酸残基の特定を行った。その結果,54位のアミノ酸残基が酵素触媒反応に関与すること,64,89,320位のアミノ酸残基は基質認識に関与することが示された。

Report

(3 results)
  • 2008 Annual Research Report   Final Research Report ( PDF )
  • 2007 Annual Research Report
  • Research Products

    (11 results)

All 2009 2008 2007

All Journal Article (8 results) (of which Peer Reviewed: 8 results) Presentation (3 results)

  • [Journal Article] The Role of Arg-96 in Danio rerio Creatine Kinase in Substrate Recognition and Active Center Configuration2009

    • Author(s)
      Uda K., Kuwasaki, A., Shima, K., Matsumoto, T. and Suzuki, T
    • Journal Title

      Int. J. Biol. Macromol (印刷中)

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] A novel arginine kinase from the shrimp Neocaridina denticulata : the fourth arginine kinase gene lineage2009

    • Author(s)
      Iwanami, K., Iseno, S., Uda K. and Suzuki, T
    • Journal Title

      Gene (印刷中)

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] The Role of Arg-96 in Danio rerio Creatine Kinase in Substrate Recogn ition and Active Center Configuration2009

    • Author(s)
      Uda K., Kuwasaki, A., Shima, K., Matsumoto, T. and Suzuki, T.
    • Journal Title

      Int. J. Biol. Macromol 413(予定)(印刷中)

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Evolution of the diverse array of phosphagen systems present in annelids2008

    • Author(s)
      Suzuki T., Uda K., Adachi M., Sanada H., Tanaka K., Mizuta C., Ishida K., Ellington W. R
    • Journal Title

      Comp. Biochem. Physiol. B. Biochemistry and Molecular Biology 152

      Pages: 60-66

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Two-domain arginine kinase from the deep-sea clam Calyptogena kaikoi-Evidence of two active domains2008

    • Author(s)
      Uda K., Yamamoto K., Iwasaki N., Iwai M., Fujikura K., Ellington W.R., Suzuki T
    • Journal Title

      Comp. Biochem. Physiol. B. Biochemistry and Molecular Biology 151

      Pages: 176-182

    • NAID

      120002126559

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Cytoplasmic and mitochondrial creatine kinases from the skeletal muscle of sperm whale (Physeter macrocephalus)2008

    • Author(s)
      Iwanami K., Uda K., Tada H., Suzuki T
    • Journal Title

      Molecular cloning and enzyme characterization.The Protein Journal 27

      Pages: 43-49

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Evolution of the cytoplasmic and mitochondrial phosphagen kinases unique to annelid groups2007

    • Author(s)
      Tanaka K., Uda K., Shimada M., Takahashi K., Gamou S., Ellington W.R.,
    • Journal Title

      J. Mol. Evol 65

      Pages: 616-625

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] A novel arginine kinase with substrate specificity toward D-arginine.2007

    • Author(s)
      Uda K.
    • Journal Title

      The Protein J 26

      Pages: 281-291

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Presentation] REACTION MECHANISM OF SABELLASTARTE ARGININE KINASE WITH SUBSTRATE SPECIFICITY TOWARDS D-ARGININE2008

    • Author(s)
      Uda K. and Suzuki T
    • Organizer
      33rd FEBS Congress & 11th IUBMB Conference
    • Place of Presentation
      アテネ(ギリシア)
    • Year and Date
      2008-07-01
    • Related Report
      2008 Annual Research Report 2008 Final Research Report
  • [Presentation] ケヤリ・アルギニンキナーゼの基質認識機構の解明2007

    • Author(s)
      宇田幸司
    • Organizer
      日本動物学会第78回弘前大会
    • Place of Presentation
      弘前大学
    • Year and Date
      2007-09-21
    • Related Report
      2008 Final Research Report
  • [Presentation] ケヤリ・アルギニンキナーゼの基質認識機構の解明2007

    • Author(s)
      宇田 幸司
    • Organizer
      日本動物学会 第78回弘前大会
    • Place of Presentation
      弘前大学
    • Year and Date
      2007-09-21
    • Related Report
      2007 Annual Research Report

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Published: 2007-04-01   Modified: 2016-04-21  

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