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Analysis of reproductive isolation by sex pheromones in unicellular conjugatophycean Closterium

Research Project

Project/Area Number 19770068
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Biodiversity/Systematics
Research InstitutionJapan Women's University

Principal Investigator

TSUCHIKANE Yuki  Japan Women's University, 理学部, 学術研究員 (20434152)

Project Period (FY) 2007 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥3,870,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥570,000)
Fiscal Year 2009: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2007: ¥1,400,000 (Direct Cost: ¥1,400,000)
Keywordsミカヅキモ / 性フェロモン / 有性生殖 / 生殖隔離 / 種分化 / ヘテロタリック / 接合藻 / 緑藻
Research Abstract

The Closterium peracerosum-strigosum-littorale (C. psl.) complex is a unicellular charophycean alga and their sex pheromones, responsible for the progress of the sexual reproduction, are well characterized. The C. psl. complex is known to be composed of several reproductively isolated mating groups of heterothallic strains. In this study, we assessed the phylogenetic relationships among reproductively isolated mating groups of the C. psl. complex and sex pheromones. Cross-reactivities of sex pheromones among the groups are also analyzed. The existing degree of reproductive isolation can be explained by differences in molecular structures and physiological activities of sex pheromones of these heterothallic mating groups.

Report

(4 results)
  • 2009 Annual Research Report   Final Research Report ( PDF )
  • 2008 Annual Research Report
  • 2007 Annual Research Report
  • Research Products

    (42 results)

All 2010 2009 2008 2007 Other

All Journal Article (6 results) (of which Peer Reviewed: 6 results) Presentation (30 results) Book (2 results) Remarks (4 results)

  • [Journal Article] Sexual processes and phylogenetic relationships of homothallic strain of the Closterium peracerosum-strigosum-littorale complex (Zygnematales, Charophyceae)2010

    • Author(s)
      Yuki Tsuchikane, Machiko Sato, Tomoko Ootaki, Yume Kokubun, Hisayoshi Nozaki, Motomi Ito, Hiroyuki Sekimoto
    • Journal Title

      Journal of Phycology 46(2)

      Pages: 274-284

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Sexual processes and phylogenetic relationships of homothallic strain of the Closterium peracerosum-strigosum-littorale complex (Zygnematales, Charophyceae).2010

    • Author(s)
      Yuki Tsuchikane, Machiko Sato, Tomoko Ootaki, Yume Kokubun, Hisayoshi Nozaki, Motomi Ito, Hiroyuki Sekimoto
    • Journal Title

      Journal of Phycology 45

      Pages: 274-284

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A taxonomic study of Eudorina elegans (Volvocaceae, Chlorophyceae) from Taiwan, based on morphology and molecular phylogeny2008

    • Author(s)
      Toshihiro K. Yamada, Yuki Tsuchikane, Jiunn-Tzong Wu, Hiroyuki Sekimoto, Kazuyuki Miyaji, Hisayoshi Nozaki
    • Journal Title

      HIKOBIA 15

      Pages: 135-143

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Reproductive isolation by sex pheromones in the Closterium peracerosum-strigosum-littorale complex (Zygnematales, Charophyceae)2008

    • Author(s)
      Yuki Tsuchikane, Motomi Ito, Hiroyuki Sekimoto
    • Journal Title

      Journal of Phycology 44(5)

      Pages: 1197-1203

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Reproductive isolation by sex pheromones in the Closterium peracerosum-strigosum-littorale complex (Zygnematales, Charophyceae)2008

    • Author(s)
      Yuki Tsuchikane, Motomi Ito and Hiroyuki Sekimoto
    • Journal Title

      Journal of Phycology 44

      Pages: 1197-1203

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A taxonomic study of Eudorina elegans (Volvocaceae, Chlorophyceae) from Taiwan, based on morphology and molecular phylogeny2008

    • Author(s)
      Toshihiro K. Yamada, Yuki Tsuchikane, Jiunn-Tzong Wu, Hiroyuki Sekimoto, Kazuyuki Miyaji, and Hisayoshi Nozaki
    • Journal Title

      HIKOBIA 15

      Pages: 135-143

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Presentation] 単細胞接合藻におけるホモタリスムの分子系統2010

    • Author(s)
      土金勇樹・加納亜子・関本弘之
    • Organizer
      日本植物分類学会第9回大会
    • Place of Presentation
      愛知教育大学(愛知県刈谷市)
    • Year and Date
      2010-03-26
    • Related Report
      2009 Annual Research Report
  • [Presentation] 接合藻ヒメミカヅキモにおけるホモタリック株の解析:ヘテロタリック株との接合実験から明らかになった「性」の存在2010

    • Author(s)
      土屋美紀・土金勇樹・関本弘之
    • Organizer
      日本植物分類学会第9回大会
    • Place of Presentation
      愛知教育大学(愛知県刈谷市)
    • Year and Date
      2010-03-26
    • Related Report
      2009 Annual Research Report
  • [Presentation] 接合藻ヒメミカツキモのホモタリック株における「性」の発見2010

    • Author(s)
      土金勇樹・土屋美樹・日向淑恵・関本弘之
    • Organizer
      日本藻類学会第34回大会
    • Place of Presentation
      筑波大学(茨城県つくば市)
    • Year and Date
      2010-03-20
    • Related Report
      2009 Annual Research Report
  • [Presentation] Characterization of a novel receptor-like protein kinase relating to the sexual reproduction of a unicellular charophycean alga, Closterium peracerosum-strigosum-littorale complex2010

    • Author(s)
      Hashiba S., Marukawa Y., Ichikawa M., Nishii I., Akatsuka S., Tsuchikane Y., Abe J., Sekimoto H.
    • Organizer
      International Symposium of Cell-Cell Communication in Plant Reproduction from pollination to fertilization
    • Place of Presentation
      奈良県新公会堂(奈良県奈良市)
    • Year and Date
      2010-03-11
    • Related Report
      2009 Annual Research Report
  • [Presentation] Asymmetric reproductive isolation by sex pheromones in a unicellular charophycean alga, Closterium.2010

    • Author(s)
      Tsuchikane Y., Sekimoto H
    • Organizer
      International Symposium of Cell-Cell Communication in Plant Reproduction from pollination to fertilization
    • Place of Presentation
      奈良県新公会堂(奈良県奈良市)
    • Year and Date
      2010-03-11
    • Related Report
      2009 Annual Research Report
  • [Presentation] Characterization of a novel receptor-like protein kinase relating to the sexual reproduction of a unicellular charophycean alga, Closterium peracerosum-strigosum-littorale complex2010

    • Author(s)
      Sayuri Hashiba, Yuka Marukawa, Machiko Ichikawa, Ichiro Nishii, Satoko Akatsuka, Yuki Tsuchikane, Jun Abe, Hiroyuki Sekimoto
    • Organizer
      International Symposium of Cell-Cell Communication in Plant Reproduction from pollination to fertilization
    • Place of Presentation
      Nara, Japan
    • Related Report
      2009 Final Research Report
  • [Presentation] Asymmetric reproductive isolation by sex pheromones in a unicellular charophycean alga, Closterium2010

    • Author(s)
      Yuki Tsuchikane, Hiroyuki Sekimoto
    • Organizer
      International Symposium of Cell-Cell Communication in Plant Reproduction from pollination to fertilization
    • Place of Presentation
      Nara, Japan
    • Related Report
      2009 Final Research Report
  • [Presentation] 接合藻ヒメミカヅキモにおけるホモタリック株の解析:ヘテロタリック株との接合実験から明らかになった「性」の存在2010

    • Author(s)
      土屋美紀・土金勇樹・関本弘之
    • Organizer
      日本植物分類学会第9回大会
    • Place of Presentation
      愛知
    • Related Report
      2009 Final Research Report
  • [Presentation] 単細胞接合藻におけるホモタリスムの分子系統2010

    • Author(s)
      土金勇樹・加納亜子・関本弘之
    • Organizer
      日本植物分類学会第9回大会
    • Place of Presentation
      愛知
    • Related Report
      2009 Final Research Report
  • [Presentation] 接合藻ヒメミカッキモのホモタリック株における「性」の発見2010

    • Author(s)
      土金勇樹・土屋美樹・日向淑恵・関本弘之
    • Organizer
      日本藻類学会第34回大会
    • Place of Presentation
      つくば
    • Related Report
      2009 Final Research Report
  • [Presentation] ホモタリックな接合藻ヒメミカヅキモにおける接合調整分子の解析2009

    • Author(s)
      土金勇樹・國分夢・関本弘之
    • Organizer
      日本植物学会73回大会、(山形)
    • Place of Presentation
      山形大学(山形県山形市)
    • Year and Date
      2009-09-18
    • Related Report
      2009 Annual Research Report
  • [Presentation] Sexual processes and phylogenetic relationships of a homothallic strain in the Closterium peracerosum- strigosum-littorale complex (Zygnematales, Charophyceae)2009

    • Author(s)
      Yuki Tsuchikane, Yume Kokubun, Hiroyuki Sekimoto
    • Organizer
      9th International Phycological Congress
    • Place of Presentation
      Tokyo, Japan
    • Year and Date
      2009-08-03
    • Related Report
      2009 Final Research Report
  • [Presentation] 単細胞接合藻ヒメミカヅキモのホモタリック株における接合調整分子の発見と特性解析2009

    • Author(s)
      國分夢・土金勇樹・関本弘之
    • Organizer
      日本藻類学会第33会大会
    • Place of Presentation
      沖縄県中頭郡琉球大学千原キャンパス
    • Year and Date
      2009-03-28
    • Related Report
      2008 Annual Research Report
  • [Presentation] 接合藻ヒメミカヅキモの生殖隔離 : 性フェロモン(PR-IP)の認識低下による非対称な生殖隔離2009

    • Author(s)
      土金勇樹・関本弘之
    • Organizer
      日本植物分類学会第8回大会
    • Place of Presentation
      宮城県仙台市
    • Year and Date
      2009-03-14
    • Related Report
      2008 Annual Research Report
  • [Presentation] 単細胞接合藻ヒメミカヅキモのホモタリック株における接合調整分子の発見と特性解析2009

    • Author(s)
      國分夢・土金勇樹・関本弘之
    • Organizer
      日本藻類学会第33会大会
    • Place of Presentation
      沖縄
    • Related Report
      2009 Final Research Report
  • [Presentation] ホモタリックな接合藻ヒメミカヅキモにおける接合調整分子の解析2009

    • Author(s)
      土金勇樹・國分夢・関本弘之
    • Organizer
      日本植物学会73回大会
    • Place of Presentation
      山形
    • Related Report
      2009 Final Research Report
  • [Presentation] 接合藻ヒメミカヅキモの生殖隔離:性フェロモン(PR-IP)の認識低下による非対称な生殖隔離2009

    • Author(s)
      土金勇樹・関本弘之
    • Organizer
      日本植物分類学会第8回大会
    • Place of Presentation
      仙台
    • Related Report
      2009 Final Research Report
  • [Presentation] Characterization of two receptor-like proteins relating to the sexual reproduction of a unicellular charophycean alga, Closterium peracerosum-strigosum-littorale complex2008

    • Author(s)
      Sekimoto, H., Akatsuka, S., Ichikawa, M., Marukawa, Y., Takekawa, Y., Abe, J., and Tsuchikane, Y
    • Organizer
      The Vth Asian Pacific Phycological Forum
    • Place of Presentation
      Victoria University, Wellington, New Zealand
    • Year and Date
      2008-11-14
    • Related Report
      2008 Annual Research Report
  • [Presentation] Biological speciation in Closterium (Zygnematales, Charophyceae) : Repro ductive isolation by sex pheromones2008

    • Author(s)
      Tsuchikane Y., and Sekimoto H
    • Organizer
      The Vth Asian Pacific Phycological Forum
    • Place of Presentation
      Victoria University, Wellington, New Zealand
    • Year and Date
      2008-11-13
    • Related Report
      2008 Annual Research Report
  • [Presentation] ホモタリックな接合藻ヒメミカヅキモの系統関係と接合機構の解析2008

    • Author(s)
      土金勇樹・佐藤真知子・中原千春・国分夢・野崎久義・伊藤元己・関本弘之
    • Organizer
      日本植物学会第72回大会
    • Place of Presentation
      高知県高知市高知大学朝倉キャンパス
    • Year and Date
      2008-09-26
    • Related Report
      2008 Annual Research Report
  • [Presentation] Characterization of two receptor-like proteins relating to the sexual reproduction of a unicellular charophycean alga, Closterium peracerosum-strigosum-littorale complex2008

    • Author(s)
      Sekimoto, H., Akatsuka, S., Ichikawa, M., Marukawa, Y., Takekawa, Y., Abe, J., Tsuchikane, Y
    • Organizer
      The Vth Asian Pacific Phycological Forum
    • Place of Presentation
      Wellington, New Zealand
    • Related Report
      2009 Final Research Report
  • [Presentation] Biological speciation in Closterium (Zygnematales, Charophyceae): Reproductive isolation by sex pheromones2008

    • Author(s)
      Yuki Tsuchikane, Hiroyuki Sekimoto
    • Organizer
      he Vth Asian Pacific Phycological Forum
    • Place of Presentation
      Wellington, New Zealand
    • Related Report
      2009 Final Research Report
  • [Presentation] ホモタリックな接合藻ヒメミカヅキモの系統関係と接合機構の解析2008

    • Author(s)
      土金勇樹・佐藤真知子・中原千春・國分夢・野崎久義・伊藤元己・関本弘之
    • Organizer
      日本植物学会第72回大会
    • Place of Presentation
      高知
    • Related Report
      2009 Final Research Report
  • [Presentation] 接合藻ヒメミカヅキモの非対称な生殖隔離に関わる性フェロモン2008

    • Author(s)
      土金勇樹・山崎華奈子・伊藤元己・関本弘之
    • Organizer
      日本藻類学会第32回大会
    • Place of Presentation
      東京
    • Related Report
      2009 Final Research Report 2007 Annual Research Report
  • [Presentation] ヒメミカヅキモの有性生殖過程のタイムラプス解析2007

    • Author(s)
      佐藤真知子・土金勇樹・関本弘之
    • Organizer
      日本植物学会71回大会
    • Place of Presentation
      東京(口頭発表)
    • Related Report
      2009 Final Research Report
  • [Presentation] ホモタリックな接合藻ヒメミカヅキモの有性生殖機構2007

    • Author(s)
      土金勇樹・大滝知子・中原千春・佐藤真知子・野崎久義・伊藤元己・関本弘之
    • Organizer
      日本植物学会71回大会
    • Place of Presentation
      東京
    • Related Report
      2009 Final Research Report
  • [Presentation] 接合藻ヒメミカヅキモの性フェロモンによる生殖隔離2007

    • Author(s)
      土金勇樹・伊藤元己・関本弘之
    • Organizer
      日本植物学会71回大会
    • Place of Presentation
      東京
    • Related Report
      2009 Final Research Report
  • [Presentation] 接合藻ヒメミカヅキモの性フェロモンによる生殖隔離2007

    • Author(s)
      土金 勇樹・伊藤 元己・関本 弘之
    • Organizer
      日本植物学会71会大会
    • Place of Presentation
      東京
    • Related Report
      2007 Annual Research Report
  • [Presentation] ホモタリックな接合藻ヒメミカヅキモの有性生殖機構2007

    • Author(s)
      土金 勇樹・大滝 知子・中原 千春・佐藤 真知子・野崎 久義・伊藤 元己・関本 弘之
    • Organizer
      日本植物学会71会大会
    • Place of Presentation
      東京
    • Related Report
      2007 Annual Research Report
  • [Presentation] ヒメミカヅキモの有性生殖過程のタイムラプス解析2007

    • Author(s)
      佐藤真知子・土金勇樹・関本弘之
    • Organizer
      日本植物学会71会大会
    • Place of Presentation
      東京
    • Related Report
      2007 Annual Research Report
  • [Book] 8. 4免疫応答の利用, 細胞生物学実験法(野村港二編著)2007

    • Author(s)
      土金勇樹
    • Publisher
      朝倉書店
    • Related Report
      2009 Final Research Report
  • [Book] 「8.4 免疫応答の利用」, 『細胞生物学実験の操作法』(野村浩二 編著)2007

    • Author(s)
      土金勇樹・関本弘之
    • Publisher
      朝倉書店
    • Related Report
      2007 Annual Research Report
  • [Remarks]

    • URL

      http://web.mac.com/tsuchikane_01/tsuchikane_yuki/home.html

    • Related Report
      2009 Final Research Report
  • [Remarks]

    • URL

      http://web.mac.com/tsuchikane_01/tsuchikane_yuki/home.html

    • Related Report
      2009 Annual Research Report
  • [Remarks]

    • URL

      http://web.mac.com/tsuchikane_01/tsuchikane-yuki/home.html

    • Related Report
      2008 Annual Research Report
  • [Remarks]

    • URL

      http://web.mac.com/tsuchikane_01/tsuchikane_yuki/home.html

    • Related Report
      2007 Annual Research Report

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Published: 2007-04-01   Modified: 2016-04-21  

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