| Budget Amount *help |
¥3,870,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥570,000)
Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2008: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2007: ¥1,400,000 (Direct Cost: ¥1,400,000)
|
|---|
| Research Abstract |
The ubiquitin ligase complex SCF^<Fbs1>, which contributes to the ubiquitination of glycoproteins, is involved in the endoplasmic reticulum-associated degradation (ERAD) pathway. To elucidate the structural basis of SCF^<Fbs1> function, we determined the crystal structures of the Skp1-Fbs1 complex, the sugar binding domain (SBD) of the Fbs1-RNaseB complex and Skp1-Fbg3 complex at 2.4, 2.7 and 2.6 A resolutions, respectively. The structure of the Skp1-Fbs1 complex illustrated the SCF^<Fbs1> ubiquitin ligase model.
|
