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X-ray crystal structural analysis of vault

Research Project

Project/Area Number 19770082
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Structural biochemistry
Research InstitutionOsaka University

Principal Investigator

TANAKA Hideaki  Osaka University, 蛋白質研究所, 助教 (40346169)

Project Period (FY) 2007 – 2008
Project Status Completed (Fiscal Year 2008)
Budget Amount *help
¥3,750,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥450,000)
Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2007: ¥1,800,000 (Direct Cost: ¥1,800,000)
Keywordsvault / X線結晶構造解析 / 自然免疫 / 核酸-蛋白質複合体 / 自然免疫反応 / 脂質ラフト / 生体超分子複合体 / SPring-8 / BL44XU / ボルト / RNA-蛋白質複合体 / 核膜孔複合体 / 核一細胞質間物質輸送 / BL44U
Research Abstract

本研究において、ボルト(vault)粒子の回転対称が39回回転対称であることを明らかにした。この事実に基づいて構造解析を進めることで3.5 A分解能でボルト粒子外殻の全体構造を決定することに成功した。粒子外殻は78個のMVP (Major Vault Protein)で形成されており、粒子長軸が約670Å、胴体部分の最大直径が約400Åであった。MVPには12個のドメイン構造があり、そのうちの1つが脂質ラフトへの結合に重要であるとされるストマチンと非常によく似た構造を持つことが明らかになった。これにより、ボルトが脂質ラフトに結合する可能性を示すことができた。

Report

(3 results)
  • 2008 Annual Research Report   Final Research Report ( PDF )
  • 2007 Annual Research Report
  • Research Products

    (20 results)

All 2009 2008 2007 Other

All Journal Article (7 results) (of which Peer Reviewed: 7 results) Presentation (9 results) Remarks (4 results)

  • [Journal Article] The structure of rat liver vault at 3.5 Angstrom resolution2009

    • Author(s)
      Tanaka H, Kato K, Yamashita E, Sumizawa T, Zhou Y, Yao M, Iwasaki K, Yoshimura M and Tsukihara T
    • Journal Title

      Scinence 323

      Pages: 384-388

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] The structure of rat liver vault at 3.5 angstrom resolution2009

    • Author(s)
      Tanaka H., et.al.
    • Journal Title

      Science 323

      Pages: 384-388

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A vault ribonucleoprotein particle exhibiting 39-fold dihedral symmetry2008

    • Author(s)
      Kato K, Tanaka H, Sumizawa T, Yoshimura M, Yamashita E, Iwasaki K and Tsukihara T
    • Journal Title

      Acta Crystallogr D Biol Crystallogr. 64

      Pages: 525-531

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] A vault ribonucleoprotein particle exhibiting 39-fold dihedral symmetry2008

    • Author(s)
      Kato K., et.al.
    • Journal Title

      Acta Crystallogr D Biol Crystallogr 64

      Pages: 525-531

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A vault ribonucleoprotein particle exhibiting 19-fold dihedral symmetry2008

    • Author(s)
      Kato k. er al.
    • Journal Title

      Acta Crystallogr D Biol Crystallogr inpress

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The struchture of melon necrotic spot virus determined at 2.8 A resolution.2008

    • Author(s)
      Wada Y., et. al.
    • Journal Title

      Acta Crystallogr F Str-uct Biol Cryst Commun 64

      Pages: 8-13

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Structures of the thermophilic F1-APTase epsilon subunit suggerting ATP-regulated arm motion of its C-terminal doma-in in F1.2007

    • Author(s)
      Yagi H.et al.
    • Journal Title

      Proc Natl Acad Sci USA 04

      Pages: 11233-11238

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Presentation] X-ray structure of the vault purified from rat liver.2008

    • Author(s)
      田中秀明、加藤公児、住澤知之、山下栄樹、吉村政人、周勇、姚閔、岩崎憲治、月原冨武
    • Organizer
      日本製物物理学会
    • Place of Presentation
      福岡県福岡市(福岡国際会議場)
    • Related Report
      2008 Final Research Report
  • [Presentation] Structure of vault purified from rat liver2008

    • Author(s)
      Kato K, Tanaka H, Sumizawa T, Yamashita E, Yoshimura M, Zhou Y, Yao M, Tanaka I, Iwasaki K and Tsukihara T
    • Organizer
      IUCr (International Union of Crystallography) 2008
    • Place of Presentation
      大阪府大阪市(大阪国際会議場)
    • Related Report
      2008 Final Research Report
  • [Presentation] The structure of melon necrotic virus determined at 2.8 A resolution2008

    • Author(s)
      Wada Y, Tanaka H, Yamashita E, Ichiki-Uehara T, Omura T and Tsukihara T
    • Organizer
      IUCr (International Union of Crystallography) 2008
    • Place of Presentation
      大阪府大阪市(大阪国際会議場)
    • Related Report
      2008 Final Research Report
  • [Presentation] X-ray structure of the vault purified from rat liver2008

    • Author(s)
      田中秀明
    • Organizer
      日本生物物理学会
    • Place of Presentation
      福岡国際会議場
    • Related Report
      2008 Annual Research Report
  • [Presentation] Structure of vault purified from rat liver2008

    • Author(s)
      加藤公児
    • Organizer
      International Union of Crystallography 2008
    • Place of Presentation
      大阪国際会議場
    • Related Report
      2008 Annual Research Report
  • [Presentation] The structure of melon necrotic spot virus detetmined at 2.8 A resolution2008

    • Author(s)
      和田康史
    • Organizer
      International Union of Crystallography 2008
    • Place of Presentation
      大阪国際会議場
    • Related Report
      2008 Annual Research Report
  • [Presentation] Struture of a latge ribonucleoprotein complex2008

    • Author(s)
      加藤 公児
    • Organizer
      The joint Symposium of the 3rd International Symposium of Institutes Network and Hot Spring Harbor-Global COE Symposium
    • Place of Presentation
      大分県別府市亀の井ホテル
    • Related Report
      2007 Annual Research Report
  • [Presentation] Rotational symmetry of vault from rat liver2007

    • Author(s)
      田中 秀明
    • Organizer
      ISDSB2007
    • Place of Presentation
      東京都江戸川区タワーホール船堀
    • Related Report
      2007 Annual Research Report
  • [Presentation] ラット肝臓由来vaultのX線結晶構造解析2007

    • Author(s)
      田中 秀明
    • Organizer
      日本結晶学会2007年度年会
    • Place of Presentation
      東京工業大学大岡山キャンパス
    • Related Report
      2007 Annual Research Report
  • [Remarks] ボルトのイメージ図、ムービーなど(公開)

    • URL

      http://www.protein.osaka-u.ac.jp/crystallography/mvp/

    • Related Report
      2008 Final Research Report
  • [Remarks] ボルトに関する報道試料など(非公開)

    • URL

      http://www.protein.osaka-u.ac.jp/olabb/tsukihara/houdou/

    • Related Report
      2008 Final Research Report
  • [Remarks] ボルトのイメージ図、ムービーなど(公開)

    • URL

      http://www.protein.osaka-u.ac.jp/crystallography/mvp/

    • Related Report
      2008 Annual Research Report
  • [Remarks] ボルトに関する報道試料な(非公開)

    • URL

      http://www.protein.osaka-u.ac.jp/crystallography/houdou/index.html

    • Related Report
      2008 Annual Research Report

URL: 

Published: 2007-04-01   Modified: 2016-04-21  

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