Structural and functional studies on RNases in the RNA degradation system
Project/Area Number |
19770083
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Single-year Grants |
Research Field |
Structural biochemistry
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Research Institution | Osaka University |
Principal Investigator |
NAKAGAWA Noriko Osaka University, 大学院・理学研究科, 助教 (50379278)
|
Project Period (FY) |
2007 – 2009
|
Project Status |
Completed (Fiscal Year 2009)
|
Budget Amount *help |
¥3,960,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥660,000)
Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2007: ¥1,100,000 (Direct Cost: ¥1,100,000)
|
Keywords | RNA / RNA分解 / 酵素反応 / 立体構造 / トランスクリプトーム解析 / 相互作用解析 / 高度好熱菌 / 立体構造解析 |
Research Abstract |
Degradation of mRNA plays an essential role in regulation of gene expression in all organisms. Defects in this process lead to potentially fatal defects. mRNA degradation is mediated by the concerted action of several ribonucleases including β-CASP family proteins. This study revealed the mechanism of RNA degradation by β-CASP family proteins and found a novel pathway that involves these proteins.
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Report
(4 results)
Research Products
(33 results)
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[Journal Article] Crystal Structure of An Archaeal Cleavage and Polyadenylation Specificity Factor Subunit from Pyrococcus horikoshii2010
Author(s)
Nishida, Y., Ishikawa, H., Baba, S., Nakagawa, N., Kuramitsu, S., Masui, R.
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Journal Title
Related Report
Peer Reviewed
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[Journal Article] A Novel Single-Stranded DNA-Specific 3′-5′Exonuclease, Thermus thermophilus Exonuclease I, Is Involved in Several DNA Repair Pathways2010
Author(s)
Shimada, A., Masui, R., Nakagawa, N., Takahata, Y., Kim, K., Kuramitsu, S., Fukui, K.
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Journal Title
Nucleic Acids Res. (in press)
Related Report
Peer Reviewed
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[Journal Article] Structure of RecJ Exonuclease Defines Its Specificity for Single-Stranded DNA2010
Author(s)
Wakamatsu, T., Kitamura, Y., Kotera, Y., Nakagawa, N., Kuramitsu, S., Masui, R.
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Journal Title
J. Biol. Chem. Vol.285,No.13
Pages: 9762-9769
Related Report
Peer Reviewed
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[Journal Article] Structure of TTHA1623, A Novel Metallo-betalactamase Superfamily protein from Thermus thermophilus HB82009
Author(s)
Yamamura, A., Okada, A., Kameda, Y., Ohtsuka, J., Nakagawa, N., Ebihara, A., Nagata, K., Tanokura, M.
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Journal Title
Acta Cryst. Vol.F65,No.5
Pages: 455-459
Related Report
Peer Reviewed
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[Journal Article] Insights into Different Dependence of dNTP Triphosphohydrolase on Metal Ion Species from Intracellular Ion Concentrations in Thermus thermophilus2008
Author(s)
Kondo, N., Nishikubo, T., Wakamatsu, T., Ishikawa, H., Nakagawa, N., Kuramitsu, S., Masui, R.
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Journal Title
Extremophiles Vol.12,No.2
Pages: 217-223
Related Report
Peer Reviewed
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[Journal Article] Crystal Structure of TTHA1429, A Novel Metallobeta- lactamase Superfamily Protein from Thermus thermophilus HB82008
Author(s)
Yamamura, A., Ohtsuka, J., Kubota, K., Agari, Y., Ebihara, A., Nakagawa, N., Nagata, K., Tanokura, M.
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Journal Title
Proteins Vol.73,No.4
Pages: 1953-1057
Related Report
Peer Reviewed
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[Journal Article] Bound Nucleotide Controls the Endonuclease Activity of Mismatch Repair Enzyme MutL2008
Author(s)
Fukui, K., Nishida, M., Nakagawa, N., Masui, R., Kuramitsu, S.
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Journal Title
J. Biol. Chem. Vol.283,No.18
Pages: 12136-12145
Related Report
Peer Reviewed
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[Journal Article] Crystal Structure of a Putative DNA Methylase TTHA0409 from Thermus thermophilus HB82008
Author(s)
Morita, R., Ishikawa, H., Nakagawa, N., Kuramitsu, S., Masui, R.
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Journal Title
Proteins Vol.73,No.1
Pages: 259-264
Related Report
Peer Reviewed
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[Journal Article] Analysis of a Nuclease Activity of Catalytic Domain of Thermus thermophilus MutS2 by High-Accuracy Mass Spectrometry2007
Author(s)
Fukui, K., Takahata, Y., Nakagawa, N., Kuramitsu, S., Masui, R.
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Journal Title
Nucleic Acids Res. Vol.35,No.15
Related Report
Peer Reviewed
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[Journal Article] Crystal Structure of Family 5 Uracil-DNA Glycosylase Bound to DNA2007
Author(s)
Kosaka, H., Hoseki, J., Nakagawa, N., Kuramitsu, S., Masui, R.
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Journal Title
J. Mol. Biol. Vol.373,No.4
Pages: 839-850
Related Report
Peer Reviewed
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[Journal Article] UvrA and UvrB Enhance Mutations Induced by Oxidized Deoxyribonucleotides2007
Author(s)
Hori, M., Ishiguro, C., Suzuki, T., Nakagawa, N., Nunoshiba, T., Kuramitsu, S., Yamamoto, K., Kasai, H., Harashima, H., Kamiya, H.
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Journal Title
DNA Repair Vol.6,No.12
Pages: 1786-1793
NAID
Related Report
Peer Reviewed
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