Structural analysis of domains from human general transcription factor, TFIIE for the determination of the complete structure.
Project/Area Number |
19770090
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Single-year Grants |
Research Field |
Structural biochemistry
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Research Institution | Yokohama City University |
Principal Investigator |
OKUDA Masahiko Yokohama City University, 国際総合科学研究科, 特任助教 (60448686)
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Project Period (FY) |
2007 – 2008
|
Project Status |
Completed (Fiscal Year 2008)
|
Budget Amount *help |
¥3,700,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥300,000)
Fiscal Year 2008: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2007: ¥2,400,000 (Direct Cost: ¥2,400,000)
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Keywords | 構造生物学 / 転写 / 基本転写因子 / タンパク質 / 立体構造 / 核磁気共鳴法 / 蛋白質 / 遣伝子発現 / NMR / 遺伝子発現 |
Research Abstract |
真核生物において、タンパク質をコードする遺伝子の転写はRNAポリメラーゼIIが行うが、開始時には基本転写因子と呼ばれる少なくとも5種類のタンパク質の助けが必要となる。本研究では、ヒト由来の基本転写因子TFIIEとTFIIHに対して、これまで不明であった両者の相互作用ドメインを同定した。さらに、遊離状態と会合状態の両方の立体構造を核磁気共鳴(NMR)装置で決定し、認識機構を明らかにした。
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Report
(3 results)
Research Products
(16 results)
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[Journal Article] Structural insight into the TFIIE/TFIIH interaction : TFIIE and p53 share the binding region on TFIIH.2008
Author(s)
Okuda, M., Tanaka, A., Satoh, M., Mizuta, S., Takazawa, M., Ohkuma, Y., Nishimura, Y.
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Journal Title
EMBO J. 27
Pages: 1161-1171
Related Report
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[Journal Article] Novel structural and functional mode of a knot essential for RNA binding activity of the Esa1 presumed chromodomain.2008
Author(s)
Shimojo, H., Sano, N., Moriwaki, Y., Okuda, M., Horikoshi, M., Nishimura, Y.
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Journal Title
J. Mol. Biol. 378
Pages: 987-1001
Related Report
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[Journal Article] Structural characterization of human general transcription factor TFIIF in solution.2008
Author(s)
Akashi, S., Nagakura, S., Yamamoto, S., Okuda, M., Ohkuma, Y., Nishimura, Y.,
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Journal Title
Protein Sci. 17
Pages: 389-400
Related Report
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[Journal Article] Structural insight into the TFUE/THIE1 interaction : TFIIE and p53 share the binding region on TFIIH2008
Author(s)
Okuda, M., Tanaka, A, Satoh, M., Mizuta, S., Takazawa, M., Ohkuma, Y., Nishimura, Y.
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Journal Title
The EMBO Journal 27
Pages: 1161-1171
Related Report
Peer Reviewed
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[Journal Article] Novel structural and functional mode of a knot essential for RNA binding activity of the Esal presumed chromodomain2008
Author(s)
Shimojo, H., Sano, N., Moriwaki, Y., Okuda, M., Horikoshi, M., Nishimura, Y.
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Journal Title
Journal of Molecular Biology 378
Pages: 987-1001
Related Report
Peer Reviewed
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[Journal Article] Structural characterization of human general transcription factor TFIIF in solution2008
Author(s)
Akashi, S., Nagakura, S., Yamamoto, S., Okuda, M., Ohkuma, Y., Nishimura, Y.
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Journal Title
Protein Science 17
Pages: 389-400
Related Report
Peer Reviewed
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[Presentation] NMR Studies on the Interaction between the General Transcription Factors2008
Author(s)
Okuda, M., Tanaka, A., Satoh, M., Mizuta, S., Takazawa, M., Ohkuma, Y., Nishimura, Y.
Organizer
TFIIE and TFIIH XXIIIrd ICMRBS Conference
Place of Presentation
米国、カリフォルニア州、サンディエゴ、シェラトンホテル
Year and Date
2008-08-26
Related Report
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[Presentation] NMR Studies on the Interaction between the General Transcription Factors, TFIIE and TFIIH2008
Author(s)
Okuda, M., Tanaka, k, Satoh, M., Mizuta, S., Takazawa, M., Ohkuma, Y., Nishimura, Y.
Organizer
XXIIIrd ICMRBS Conference
Place of Presentation
米国、カリフォルニア州、サンディエゴ、シェラトンホテル
Year and Date
2008-08-26
Related Report
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