Crystallographic study of poly-γ-glutamate hydrolase.

• Project/Area Number: 19770093
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Structural biochemistry
• Research Institution: National Institute of Agrobiological Sciences
• Principal Investigator: FUJIMOTO Zui National Institute of Agrobiological Sciences, タンパク質機能研究ユニット, 主任研究員 (20370679)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥3,800,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥600,000); Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2008: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2007: ¥1,200,000 (Direct Cost: ¥1,200,000)
• Keywords: X線結晶解析 / ボリーγーグルタミン酸 / PGA酸加水分解酵素 / 納豆菌 / 酵素反応機構 / X線結晶構造解析 / 双晶 / ポリ-γ-グルタミン酸

Research Abstract

Poly-γ-glutamate hydrolase P (PghP) of Bacillus subtilis bacteriophage ΦNIT1 hydrolyzes the γ-glutamyl peptide linkage of extracellular poly-γ-glutamate produced by bacilli. Crystal structure of PghP was determined at a resolution of 1.9 A. Structure of PghP was elucidated as a globular protein with an open α/β mixed core structure and a seven-stranded parallel/anti-parallel β-sheet. Structure analysis demonstrated that PghP had a catalytic center containing a zinc ion and an overall topology resembling mammalian carboxypeptidase A and related enzymes.

Research Products

• [Journal Article] Crystallization and preliminary crystallographic analysis of poly-γ-glutamate hydrolase from bacteriophage FNIT1. (2009)
  • Author(s): Zui Fujimoto, Isao Shiga, Yoshifumi Itoh, Keitarou Kimura
  • Journal Title: Acta Crystallog. sect. F Struct. Biol. Crystalli. Commun. 65(9) Pages: 913-916
  • Peer Reviewed
• [Journal Article] Crystallization and preliminary crystallographic analysis of poly-g-glutamate hydrolase from bacteriophage FNIT1 (2009)
  • Author(s): Zui Fujimoto, Isao Shiga, Yoshifumi Itoh, Keitarou Kimura
  • Journal Title: Acta Crystallog.sect.F Struct.Biol.Crystalli.Commun. 65 Pages: 913-916
  • Peer Reviewed
• [Presentation] 納豆菌ファージのγポリグルタミン酸分解酵素PghPの構造 (2010)
  • Author(s): 木村啓太郎、藤本瑞
  • Organizer: 日本農芸化学会2010年度大会
  • Place of Presentation: 東京大学駒場キャンパス
  • Year and Date: 2010-03-29
• [Presentation] 納豆菌ファージのγポリグルタミン酸分解酵素PghPの構造 (2010)
  • Author(s): 木村啓太郎, 藤本瑞
  • Organizer: 日本農芸化学会2010年度大会講演要旨集
  • Place of Presentation: 東京
• [Presentation] バクテリオファージ由来ポリ-γ-グルタミン酸加水分解酵素の結晶構造解析における双晶データの扱い (2009)
  • Author(s): 藤本瑞、木村啓太郎
  • Organizer: 日本結晶学会2009年度大会
  • Place of Presentation: 関西学院大学上ヶ原キャンパス
  • Year and Date: 2009-12-05
• [Presentation] Bacteriophage contamination and fermentation of Natto by Bacillus subtilis(natto)-Study of phage related key enzyme that spoils sticky texture of natto- (2009)
  • Author(s): 木村啓太郎, 藤本瑞
  • Organizer: BioMicrowold 2009
  • Place of Presentation: リスボン ポルトガル
  • Year and Date: 2009-12-03
• [Presentation] 納豆菌ファージが生産するポリ-γ-グルタミン酸加水分解酵素のX線結晶構造解析 (2009)
  • Author(s): 藤本 瑞
  • Organizer: 第26回PFシンポジウム
  • Place of Presentation: つくば国際会議場
  • Year and Date: 2009-03-25
• [Presentation] 納豆菌ファージが生産するポリ-γ-グルタミン酸加水分解酵素のX線結晶構造解析 (2009)
  • Author(s): 藤本瑞, 志賀勲, 伊藤義文, 木村啓太郎
  • Organizer: 第26回PFシンポジウム要旨集
  • Place of Presentation: つくば
• [Presentation] Bacteriophage contamination and fermentation of Natto by Bacillus subtilis (natto) -Study of phage related key enzyme that spoils sticky texture of natto- (2009)
  • Author(s): Keitarou Kimura, Zui Fujimoto
  • Organizer: BioMicrowold2009
  • Place of Presentation: Lisbon Portugal
• [Presentation] バクテリオファージ由来ポリ-γ-グルタミン酸加水分解酵素の結晶構造解析における双晶データの扱い (2009)
  • Author(s): 藤本瑞、木村啓太郎
  • Organizer: 日本結晶学会2009年度大会講演要旨集
  • Place of Presentation: 西宮
• [Book] Amino-Acid Homopolymers Occurring in Nature.(Edited by Hamano, Yoshimitsu, Enzymic degradation of poly-gamma-glutamic acid. In Microbiology Monographs, vol.15)
  • Author(s): Keitarou Kimura, Zui Fujimoto
  • Publisher: Springer.(印刷中)