Characterization of the tRNA-s2T thiolation enzymes required for cell growth at extremely high temperatures

• Project/Area Number: 19770096
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Structural biochemistry
• Research Institution: National Institute of Advanced Industrial Science and Technology
• Principal Investigator: SHIGI Naoki National Institute of Advanced Industrial Science and Technology, バイオメディシナル情報研究センター, 研究員 (20392623)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,670,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥270,000); Fiscal Year 2008: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2007: ¥2,500,000 (Direct Cost: ¥2,500,000)
• Keywords: 核酸 / tRNA / タンパク質合成系 / 硫黄 / 補酵素 / 転移RNA / 好熱菌 / 生合成 / タンパク質合成 / 酵素 / 分子生物学

Research Abstract

高度好熱菌tRNA を耐熱化する働きをもつ硫黄化修飾の生合成機構の解析を行った。新規生合成因子を同定し、組換えタンパク質を用いて試験管内で反応機構を解析した。その結果この硫黄化修飾の生合成は新規中間体(チオカルボキシレート)を経由する新しいタイプのtRNA 硫黄化反応系であることが判明した。この系はモリブデン補酵素やチアミンなどの硫黄を含む補酵素の生合成系と共通の祖先から進化したと考えられる。

Research Products

• [Journal Article] Common thiolation mechanism in the biosynthesis of tRNA thiouridine and sulphur-containing cofactors (2008)
  • Author(s): 鴫 直樹、坂口裕理子、朝井真一、鈴木勉、渡辺公綱
  • Journal Title: The EMBO Journal 27 巻 Pages: 3267-3278
  • Peer Reviewed
• [Journal Article] Common thiolation mechanism in the biosynthesis of tRNA thiouridine and sulphur-containing cofactors (2008)
  • Author(s): 鴫直樹
  • Journal Title: The EMBO Journal 27 Pages: 3267-3278
  • Peer Reviewed
• [Journal Article] Common thiolation mechanism in the biosynthesis of tRNA thiouridine and sulphur-containing cofactors (2008)
  • Author(s): 鴫 直樹
  • Journal Title: The EMBO Journal 27 Pages: 3267-3278
  • Peer Reviewed
• [Presentation] Common thiolation mechanism in the biosynthesis of transfer RNA thiouridine and sulphur-containing cofactors (2009)
  • Author(s): 鴫 直樹ら
  • Organizer: EMBO-FEMS workshop on: Microbial sulfur metabolism
  • Place of Presentation: トマール(ポルトガル)
  • Year and Date: 2009-03-16
• [Presentation] Common thiolation mechanism in the biosynthesis of transfer RNA thiouridine and sulphur-containing cofactors (2009)
  • Author(s): 鴫直樹
  • Organizer: EMBO-FEMS workshop on : Microbial sulfur metabolism
  • Place of Presentation: トマール(ポルトガル)
  • Year and Date: 2009-03-16
• [Presentation] Characterization of the tRNA-s2T thiolation enzymes required for cell growth at extremely high temperatures (2008)
  • Author(s): 鴫 直樹ら
  • Organizer: RNA2008
  • Place of Presentation: ベルリン(ドイツ)
  • Year and Date: 2008-08-01
• [Presentation] Characterization of the tRNA-s2T thiolation enzymes required for cell growth at extremely high temperatures (2008)
  • Author(s): 鴫 直樹
  • Organizer: RNA2008
  • Place of Presentation: ベルリン (ドイツ)
  • Year and Date: 2008-08-01
• [Presentation] 高度好熱菌タンパク質合成系を耐熱化するtRNA の硫黄修飾の生合成機構 (2007)
  • Author(s): 鴫 直樹ら
  • Organizer: BMB2007 日本分子生物学会・日本生化学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-13
• [Presentation] 高度好熱菌タンパク質合成系を耐熱化するtRNAの硫黄修飾の生合成機構 (2007)
  • Author(s): 鴫 直樹
  • Organizer: BMB2007 日本分子生物学会・日本生化学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-13
• [Presentation] Characterization of the tRNA-s2T thiolation enzymes required for cell growth at extremely high temperatures (2007)
  • Author(s): 鴫 直樹ら
  • Organizer: 22nd tRNA workshop
  • Place of Presentation: ウプサラ(スウェーデン)
  • Year and Date: 2007-11-04
• [Presentation] Transfer RNA methylation in Thermus thermophilus (2007)
  • Author(s): 堀 弘幸ら
  • Organizer: 22nd tRNA workshop
  • Place of Presentation: ウプサラ(スウェーデン)
  • Year and Date: 2007-11-04
• [Presentation] Characterization of the tRNA-s2T thiolation enzymes required for cell growth at extremely high temperatures (2007)
  • Author(s): 鴫 直樹
  • Organizer: 22^<nd> tRNA workshop
  • Place of Presentation: ウプサラ (スウェーデン)
  • Year and Date: 2007-11-04
• [Presentation] Transfer RNA methylation in Thermus thermophilus (2007)
  • Author(s): 堀 弘幸
  • Organizer: 22^<nd> tRNA workshop
  • Place of Presentation: ウプサラ (スウェーデン)
  • Year and Date: 2007-11-04
• [Presentation] 高度好熱菌tRNA に耐熱性を付与する修飾塩基s2T の生合成機構 (2007)
  • Author(s): 鴫 直樹ら
  • Organizer: 第9回 日本RNA 学会年会
  • Place of Presentation: 名古屋
  • Year and Date: 2007-07-30
• [Presentation] 高度好熱菌tRNAに耐熱性を付与する修飾塩基s2Tの生合成機構 (2007)
  • Author(s): 鴫 直樹
  • Organizer: 第9回 日本RNA学会年会
  • Place of Presentation: 名古屋
  • Year and Date: 2007-07-30
• [Book] DNA and RNA modification enzymes (2009)
  • Author(s): 野間章子
  • Publisher: Landes Bioscience
• [Book] Biogenesis and functions of thio-compounds inDNA and RNA modification enzymes (transfer RNA: comparison of bacterial and eukaryotic thiolation machineries)
  • Author(s): 野間章子、鴫 直樹、鈴木 勉
  • Publisher: Landes Bioscience