Analysis of complement activation in MASP-deficient mice

• Project/Area Number: 19770113
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Functional biochemistry
• Research Institution: Fukushima Medical University
• Principal Investigator: IWAKI Daisuke Fukushima Medical University, 医学部, 助教 (10315492)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥3,930,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥630,000); Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2008: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2007: ¥1,200,000 (Direct Cost: ¥1,200,000)
• Keywords: 自然免疫 / 補体 / レクチン / セリンプロテアーゼ

Research Abstract

MASP-1 and MASP-2 are known to cleave some complement components and contribute to activation of the complement system. On the other hand, the function of MASP-3 remains unclear. To further clarify the roles of MASPs we generated and analyzed 3 types of MASP-deficient mice. In this study, we found a way to activate MASP-3 and the protease might be involved in activation of the alternative complement pathway.

Research Products

• [Journal Article] Essential role of mannose-binding lectin-associated serine protease-1 in activation of the complement factor (2010)
  • Author(s): M. Takahashi, Y. Ishida, D. Iwaki, K. Kanno, T. Suzuki, Y. Endo, Y. Homma, T. Fujita
  • Journal Title: D.J. Exp. Med. 207 Pages: 29-37
  • Peer Reviewed
• [Journal Article] Essential role of mannose-binding lectin-associated serine protease-1 in activation of the complement factor D. (2010)
  • Author(s): Minoru Takahashi
  • Journal Title: J.Exp.Med. 207 Pages: 29-37
  • Peer Reviewed
• [Journal Article] Interactions of ficolin and mannose-binding lectin with fibrinogen/fibrin augment the lectin complement pathway. (2009)
  • Author(s): Y. Endo, N. Nakazawa, D. Iwaki, M. Takahashi, M. Matsushita, T. Fujita
  • Journal Title: J. Innate. Immun. 2 Pages: 33-42
  • Peer Reviewed
• [Journal Article] Interactions of ficolin and mannose-binding lectin with fibrinogen/fibrin augment the lectin complement pathway. (2009)
  • Author(s): Yuichi Endo
  • Journal Title: J.Innate.Immun. 2 Pages: 33-42
  • Peer Reviewed
• [Journal Article] Mannose-binding lectin (MBL)-associated serine protease (MASP)-1 contributes to activation of the lectin complement pathway. (2008)
  • Author(s): M. Takahashi, D. Iwaki, K. Kanno, Y. Ishida, J. Xiong, M. Matsushita, Y. Endo, S. Miura, N. Ishii, K. Sugamura, T. Fujita
  • Journal Title: J. Immunol. 180 Pages: 6132-6138
  • Peer Reviewed
• [Journal Article] Plasma UGRP1 levels associate with promoter G-112A polymorphism and the severity of asthma. (2008)
  • Author(s): K. Inoue, X. Wang, J. Saito, Y. Tanino, T. Ishida, D. Iwaki, T. Fujita, S. Kimura, M. Munakata
  • Journal Title: Allergol. Int. 57 Pages: 57-64
  • NAID: 10021246095
  • Peer Reviewed
• [Journal Article] Mannose-binding lectin (MBL)-associated serine protease(MASP)-1 contributes to activation of the lectin complement pathway (2008)
  • Author(s): Minoru Takahashi
  • Journal Title: J. Immunol 180・9 Pages: 6132-6138
  • Peer Reviewed
• [Journal Article] Plasma UGRP1 levels associate with promoter G-112A polymorphism and the severity of asthma (2008)
  • Author(s): Keiichi Inoue
  • Journal Title: Allergol. Int. 57・1 Pages: 57-64
  • Peer Reviewed
• [Journal Article] 補体活性化のレクチン経路とプロテアーゼ (2007)
  • Author(s): 岩城大輔、藤田禎三
  • Journal Title: 臨床免疫・アレルギー科 48巻 Pages: 711-718
  • NAID: 40015794098
• [Journal Article] 補体活性化のレクチン経路とプロテアーゼ (2007)
  • Author(s): 岩城 大輔
  • Journal Title: 臨床・免疫アレルギー科 48・6 Pages: 711-718
  • NAID: 40015794098
• [Presentation] Mannose-binding lectin-associated serine protease 3 (MASP-3)の機能解析 (2008)
  • Author(s): 岩城大輔、菅野和子、高橋実、遠藤雄一、松下操、藤田禎三
  • Organizer: BMB2008(第31回日本分子生物学会年会・第81回日本生化学会大会合同大会)
  • Place of Presentation: 兵庫県神戸市
  • Year and Date: 2008-12-10
• [Presentation] Mannose-binding lectin-associated serone protease 3(MASP-3)の機能解析 (2008)
  • Author(s): 岩城大輔
  • Organizer: BMB2008(第31回日本分子生物学会年会・第81回日本生化学会大会合同大会)
  • Place of Presentation: 兵庫県神戸市
  • Year and Date: 2008-12-10
• [Presentation] Activation of mannose-binding lectin-associated serine protease 3. (2008)
  • Author(s): D. Iwaki, K. Kanno, M. Takahashi, Y. Endo, M. Matsushita, T. Fujita
  • Organizer: XXII International Complement Workshop, Basel
  • Place of Presentation: Switzerland
  • Year and Date: 2008-09-30
• [Presentation] Activation of mannose-binding lectin-associated serine protease 3 (2008)
  • Author(s): Daisuke Iwaki
  • Organizer: XXII International Complement Workshop
  • Place of Presentation: Basel, Switzerland
  • Year and Date: 2008-09-30
• [Presentation] MASP-3の活性化と機能解析 (2008)
  • Author(s): 岩城大輔、菅野和子、高橋実、遠藤雄一、松下操、藤田禎三
  • Organizer: 第73回インターフェロン・サイトカイン学会第19回生体防御学会第45回補体シンポジウム3学会合同大会
  • Place of Presentation: 北海道札幌市
  • Year and Date: 2008-07-11
• [Presentation] MASP欠損マウスにおけるStaphylococcus aureusに対する食作用の解析 (2007)
  • Author(s): 岩城大輔、菅野和子、高橋実、遠藤雄一、松下操、藤田禎三
  • Organizer: 第44回補体シンポジウム
  • Place of Presentation: 神奈川県平塚市
  • Year and Date: 2007-08-24