Kinetic and X-ray crystallographic analysis of quantum mechanical hydrogen tunneling in enzyme-catalyzed reaction

• Project/Area Number: 19770118
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Functional biochemistry
• Research Institution: Osaka Medical College
• Principal Investigator: MURAKAWA Takeshi Osaka Medical College, 医学部, 助教 (90445990)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥3,890,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥690,000); Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2008: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2007: ¥900,000 (Direct Cost: ¥900,000)
• Keywords: プロトントンネリング / ビルトインキノン補酵素 / 同位体効果 / ビルトイン補酵素 / トパキノン

Research Abstract

It is becoming widely accepted that the hydrogen tunneling is a general feature of the enzyme-catalyzed reaction at room temperature, especially in the reaction requiring high activation energy such as C-H bond cleavage. We analyzed stereospecific abstraction of substrate ・^-proton catalyzed by copper amine oxidases from Arthrobacter globiformis (AGAO) by transition-state kinetics and X-ray crystallographic analysis. These results provide new finding for enzyme-enhanced hydrogen tunneling.

Research Products

• [Journal Article] Further insight into the mechanism of streoselective proton abstraction by bacterial copper amine oxidase (2008)
  • Author(s): Masayasu Taki, Takeshi Murakawa (equally contributing), Takuya Nakamoto, Mayumi Uchida, Hideyuki Hayashi, Katsuyuki Tanizawa, Yukio Yamamoto, Toshihide Okajima
  • Journal Title: Biochemistry 47 Pages: 7726-7733
  • Peer Reviewed
• [Journal Article] Further insight into the mechanism of streoselective proton abstraction by bacterial copper amine oxidase (2008)
  • Author(s): Masayasu Taki, Takeshi Murakawa (equally contributing)
  • Journal Title: Biochemistry 47 Pages: 7726-7733
  • Peer Reviewed
• [Presentation] Acid-Base Chemistry in the Reductive Half-Reaction of Copper Amine Oxidase from Arthrobacter globiformis (2010)
  • Organizer: Gordon Research Conference
  • Place of Presentation: Ventura (USA)
• [Presentation] Acid-Base Chemistry in the Reductive Half-Reaction of Copper Amine Oxidase from Arthrobacter globiformis (2010)
  • Author(s): 村川武志
  • Organizer: Gordon Research Conference
  • Place of Presentation: Ventura(USA)
• [Presentation] 速度論および構造解析に基づく酵素触媒反応におけるプロトントンネリング機構の解析 (2009)
  • Organizer: 日本生化学会
  • Place of Presentation: 神戸
  • Year and Date: 2009-10-22
• [Presentation] 速度論および構造解析に基づく酵素触媒反応におけるプロトントンネリング機構の解析 (2009)
  • Author(s): 村川武志
  • Organizer: 日本生化学会
  • Place of Presentation: 神戸ポートアイランド(兵庫)
  • Year and Date: 2009-10-22
• [Presentation] 速度論および構造解析に基づく酵素反応触媒反応におけるプロトントンネリング機構の解析 (2008)
  • Author(s): 村川武志
  • Organizer: 日本生化学会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-09
• [Presentation] Hydrogen Tunneling in Bacterial Copper Amine Oxidase Reaction (2008)
  • Author(s): 村川武志
  • Organizer: Gordon Research Conference
  • Place of Presentation: Ventura (USA)