Fabrication of stretching DNA chip for a single molecule analysis of DNA binding proteins

• Project/Area Number: 19770124
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Biophysics
• Research Institution: Hokkaido University
• Principal Investigator: MATSUO Yasutaka Hokkaido University, 電子科学研究所, 助教 (90374652)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥2,870,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥270,000); Fiscal Year 2008: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2007: ¥1,700,000 (Direct Cost: ¥1,700,000)
• Keywords: 1分子計測 / 操作 / ナノバイオ / 生物物理 / 核酸 / 1分子計測(SMD) / 表面・界面物性

Research Abstract

微細な凹凸構造をもつ基板上にDNA を伸長固定化することでDNA 結合タンパク質が自由な環境下でDNA と相互作用が可能となるDNA チップの作製を試みた。研究成果としては光リソグラフィー技術を用いたシリコン基板上への微小な凹凸構造作製、また高分子フィルム上への凹凸構造を作製することにより、様々な基板へのDNA の伸長固定化条件の抽出を行った。これによりDNAとDNA結合タンパク質の相互作用解析用基板の作製を可能にした。

Research Products

• [Journal Article] Photo-induced fluorescence emission enhancement of azobenzene thin films (2008)
  • Author(s): O. Haruta, Y. Matsuo, K. Ijiro
  • Journal Title: Colloids and Surfaces A-Physicochemical and Engineering Aspects 313 Pages: 595-599
  • NAID: 120000952607
  • Peer Reviewed
• [Journal Article] Sequence-Specific Control of Azobenzene Assemblies by Molecular Recognition of DNA (2008)
  • Author(s): O. Haruta, Y. Matsuo, Y. Hashimoto, K.Niikura, K.Ijiro
  • Journal Title: Langmuir 24 Pages: 2618-2624
  • Peer Reviewed
• [Journal Article] Stabilization of DNA multiassembly by addition of a phosphate group at the 5 '-sticky end (2008)
  • Author(s): A. Tanaka, Y. Matsuo, K. Niikura, K. Ijiro
  • Journal Title: Chemistry Letters 37 Pages: 758-759
  • NAID: 10021082492
  • Peer Reviewed
• [Journal Article] Sequence-specifically platinum metal deposition on enzymatically synthesized DNA block copolymer (2008)
  • Author(s): A. Tanaka, Y. Matsuo, Y. Hashimoto and K. Ijiro
  • Journal Title: Chem. Commun 36 Pages: 4270-4272
  • Peer Reviewed
• [Journal Article] Stabilization of DNA multiassembly by addition of a phosphate group at the 5 '-sticky end (2008)
  • Author(s): A. Tanaka
  • Journal Title: Chemistry Letters 37 Pages: 758-759
  • Peer Reviewed
• [Journal Article] Sequence-specifically platinum metal deposition on enzymatic ally synthesized DNA block copolymer (2008)
  • Author(s): A. Tanaka
  • Journal Title: Chem. Commun 36 Pages: 4270-4272
  • Peer Reviewed
• [Journal Article] Sequence-Specific Control of Azobenzene Assemblies by Molecular Recognition of DNA (2008)
  • Author(s): O. Haruta
  • Journal Title: Langmuir 24 Pages: 2618-2624
  • Peer Reviewed
• [Journal Article] 高分子科学の最近の進歩「高分子と金属の複合化と高機能創製 (2007)
  • Author(s): 松尾保孝, 居城邦治
  • Journal Title: 高分子10月号 Pages: 845-849
• [Presentation] DNA を鋳型とした金属ナノ構造体の形成制御 (2008)
  • Author(s): 居城邦治, 田中あや, 石川綾子, 新倉謙一, 松尾保孝
  • Organizer: 第57回高分子討論会
  • Place of Presentation: 大阪市立大学
  • Year and Date: 2008-09-25
• [Presentation] DNA の塩基配列選択的なプラチナナノワイヤーの作製 (2008)
  • Author(s): 松尾保孝, 田中あや, 居城邦治
  • Organizer: 第3回バイオ関連化学合同シンポジウム
  • Place of Presentation: 東京工業大学
  • Year and Date: 2008-09-20
• [Presentation] DNAの塩基配列に選択的なプラチナナノワイヤーの作製 (2008)
  • Author(s): 松尾保孝
  • Organizer: 生体機能関連化学シンポジウム
  • Place of Presentation: 東京工業大学
  • Year and Date: 2008-09-20
• [Presentation] Synthesis of silver metallic nanoparticles complexed with DNA by photoreduction (2008)
  • Author(s): 石川綾子, 松尾保孝, 居城邦治
  • Organizer: Internatinal conference photoexcited process and applications
  • Place of Presentation: Sapporo
  • Year and Date: 2008-09-10
• [Presentation] Synthesis of silver metallic nanoparticles complexed with DNA by photo reduction (2008)
  • Author(s): 松尾保孝
  • Organizer: International conference of photo-excited process a nd applications
  • Place of Presentation: 札幌ロイトン札幌
  • Year and Date: 2008-09-09
• [Presentation] Silver/oligonucleotides hybrid nanoparticles prepared by photoreduction (2007)
  • Author(s): 佐藤壮人, 石川綾子, 松尾保孝, 居城邦治
  • Organizer: 2007 Korea-Japan Symposium on Frontier Photoscience
  • Place of Presentation: Gyeongju,Korea
  • Year and Date: 2007-11-22
• [Presentation] DNA を用いた発光性銀ナノ粒子の作製とバイオイメージングへの応用 (2007)
  • Author(s): 松尾保孝, 石川綾子, 佐藤壮人, 居城邦治
  • Organizer: 生体機能関連化学部会
  • Place of Presentation: 東北大学多元物質科学研究所
  • Year and Date: 2007-09-29
• [Presentation] DNA でコートした銀ナノ粒子の作製と光励起発光現象 (2007)
  • Author(s): 佐藤壮人, 石川綾子, 松尾保孝, 居城邦治
  • Organizer: 第59回コロイドおよび界面化学討論会
  • Place of Presentation: 信州大学
  • Year and Date: 2007-09-22
• [Presentation] オリゴヌクレオチド/銀ハイブリッドナノ粒子の発光特性解析 (2007)
  • Author(s): 佐藤壮人, 石川綾子, 松尾保孝, 居城邦治
  • Organizer: 第56回高分子討論会
  • Place of Presentation: 名古屋工業大学
  • Year and Date: 2007-09-20
• [Presentation] DNA-metal hybrid nanomaterials (2007)
  • Author(s): 居城邦治, 佐藤壮人, 新倉謙一, 松尾保孝
  • Organizer: SPIE Optics+photonics 2007
  • Place of Presentation: San Diego Convention Center, San Diego, California USA
  • Year and Date: 2007-08-27
• [Presentation] DNA-metal hybrid nanomaterials (2007)
  • Author(s): Y. Matsuo
  • Organizer: SPIE Optics+Photonics 2007
  • Place of Presentation: San Diego Convention Center, San Diego, Calif ornia USA
  • Year and Date: 2007-08-27
• [Presentation] Photoluminescence of silver nanoparticles complexed with DNA (2007)
  • Author(s): 松尾保孝, 佐藤壮人, 居城邦治
  • Organizer: The 13th International Conferencde on Photochemistry
  • Place of Presentation: Cologne, Germany
  • Year and Date: 2007-07-29
• [Presentation] DNA と複合化した銀ナノ粒子の発光挙動 (2007)
  • Author(s): 佐藤壮人, 松尾保孝, 居城邦治
  • Organizer: 第56回高分子年次大会
  • Place of Presentation: 国立京都国際会館
  • Year and Date: 2007-05-30
• [Presentation] DNA を用いた金属ナノ材料の作製 (2007)
  • Author(s): 松尾保孝
  • Organizer: 第2回東北大学多元物質研究所若手講演会
  • Place of Presentation: 東北大学多元物質研究所
  • Year and Date: 2007-05-15
• [Book] 金属・マイクロ粒子の形状・構造制御技術、第7章 (2009)
  • Author(s): 居城邦治, 新倉謙一, 松尾保孝
  • Publisher: シーエムシー出版
• [Book] ナノバイオ大事典-COMPREHENSIVE NANO-BIO HANDBOOK-LB(Langmuir-Blodgett)膜 (2007)
  • Author(s): 居城邦治
  • Publisher: (株)テクノシステム・東京
• [Remarks]
  • URL: http://chem.es.hokudai.ac.jp/mdl/