Molecular mechanism of chaperone network

• Project/Area Number: 19770137
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Biophysics
• Research Institution: The Institute of Physical and Chemical Research
• Principal Investigator: ZAKO Tamotsu The Institute of Physical and Chemical Research, 前田バイオ工学研究室, 専任研究員 (50399440)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,790,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥390,000); Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2007: ¥2,100,000 (Direct Cost: ¥2,100,000)
• Keywords: 好熱菌タンパク質 / 分子シャペロン / シャペロニン / プレフォルディン / 分子間相互作用 / リフォールディング / 蛍光偏光異方性解消法 / 変性タンパク質 / アミロイド / アップコンバージョンナノ粒子

Research Abstract

シャペロニン(CPN)はATP依存的に基質である変性タンパク質をリフォールディングする代表的なシャペロンである。一方プレフォルディン(PFD)は変性タンパク質を捕捉し、CPNへ受け渡すと考えられている.本研究では、ATPアナログを用いてCPNの構造変化サイクルを人為的に止め、PFDとの相互作用解析をおこなった.その結果、ADP状態(OPEN構造)のCPNに最も強く結合し基質タンパク質を受け渡していることが明らかとなった.

Research Products

• [Journal Article] Improvement of dispersion stability and characterization of upconversion nanophosphors covalently modified with PEG as a fluorescence bioimaging probe (2008)
  • Author(s): Zako, T., Nagata, H., Terada, N., Sakono, M, Soga, K and Maeda, M.
  • Journal Title: J. Materials Sci. 43 Pages: 5325-30
  • Peer Reviewed
• [Journal Article] Effect of the C-terminal truncation on the functional cycle of chaperonin groel : Implication that the C-terminal region facilitates the transition from the folding-arrested to the folding-competent state. (2008)
  • Author(s): Suzuki M, Ueno T, Iizuka R, Miura T, Zako T, Akahori R, Miyake T, Shimamoto N, Aoki M, Tanii T, Ohdomari I, Funatsu T.
  • Journal Title: J. Biol. Chem. 283 Pages: 23931-39
  • Peer Reviewed
• [Journal Article] Kinetic Analysis of Conformational Changes of GroEL Based on the Fluorescence of Tyrosine 506. (2008)
  • Author(s): Hosono K, Ueno T, Taguchi H, Motojima F, Zako T, Yoshida M, Funatsu T.
  • Journal Title: Protein J. 27 Pages: 461-8
  • Peer Reviewed
• [Journal Article] Dynamics of group II chaperonin and prefoldin probed by 13C NMR spectroscopy (2008)
  • Author(s): Kurimoto, E., Nishi, Y., Yamaguchi, Y., Zako, T., Iizuka, R., Ide, N., Yohda, M. and Kato, K.
  • Journal Title: Proteins 70 Pages: 1257-63
  • Peer Reviewed
• [Journal Article] Improvement of dispersion stability and characterization of upcbnversion nanophosphors covalently modified with PEG as a fluorescence bioimaging probe (2008)
  • Author(s): Zako, T.
  • Journal Title: Journal of Materials Science 43 Pages: 5325-5330
  • Peer Reviewed
• [Journal Article] Formation of highly toxic soluble amyloid beta oligomers by the molecular chaperone prefoldin (2008)
  • Author(s): Sakono, M.
  • Journal Title: FEBS Journal 275 Pages: 5982-5993
  • Peer Reviewed
• [Journal Article] Effect of the C-terminal truncation on the functional cycle of chaperonin groel (2008)
  • Author(s): Suzuki, M.
  • Journal Title: Journal of Biological Chemistry 283 Pages: 23931-23939
  • Peer Reviewed
• [Journal Article] Kinetic Analysis of Conformational Changes of GroEL Based on the Fluorescence of Tyrosine 506 (2008)
  • Author(s): Hosono, K.
  • Journal Title: Protein Journal 27 Pages: 461-468
  • Peer Reviewed
• [Journal Article] Dynamics of group II chaperonin and prefoldin probed by (13)C NMR spectroscopy (2008)
  • Author(s): Kurimoto, E.
  • Journal Title: Proteins 70 Pages: 1257-1263
  • Peer Reviewed
• [Journal Article] Selectivity improvement in protein nanopatterning with a hydroxy-terminated self-assembled monolayer template (2007)
  • Author(s): Miyake, T, Tanii, T., Kato, K., Zako, T., Funatsu, T. and Ohdomari I.
  • Journal Title: Nanotechnology 18 Pages: 305304-305304
  • Peer Reviewed
• [Journal Article] Complex formation of CdSe/ZnS/TOPO nanocrystal vs. molecular chaperone in aqueous solution by hydrophobic interaction (2007)
  • Author(s): Horiuchi, H., Iwami, N., Tachibana, F., Ohtaki, A., Iizuka, R., Zako, T., Oda, M., Yohda, M. and Tani, T.
  • Journal Title: J. Luminescence 127 Pages: 192-197
  • Peer Reviewed
• [Journal Article] Selectivity improvement in protein nanopatterning with a hydroxy-terminated self-assembled monolayer template (2007)
  • Author(s): Miyake, T.
  • Journal Title: Nanotechnology 18 Pages: 304-305
  • Peer Reviewed
• [Journal Article] Complex formation of CdSe/ZnS/TOPO nanocrystal vs. molecular chaperone in aqueous solution by hydrophobic interaction (2007)
  • Author(s): Horiuchi, H.
  • Journal Title: Journal of Luminescence 127 Pages: 192-197
  • Peer Reviewed
• [Journal Article] Formation of highly toxic soluble amyloid beta oligomers by the molecular chaperone prefoldin
  • Author(s): Sakono, M., Zako, T., Ueda, H., Yohda, M. and Maeda, M.
  • Journal Title: FEBS Journal (in press)
  • Peer Reviewed
• [Journal Article] Dynamics of group II chaperonin and prefoldin probed by (13)C NMR spectroscopy
  • Author(s): Kurimoto, E.
  • Journal Title: Proteins In press
  • Peer Reviewed
• [Presentation] Detection and diagnostics of amyloidic proteins using luminescent conjugated polymers (2008)
  • Author(s): Mikael Lindgren, Tamotsu Zako, Mizuo Maeda, Per Hammarstrom and K. Peter R. Nilsson
  • Organizer: Joint Meeting of the Japanese Biochemical Society and the Molecular Biology Society of Japan
  • Place of Presentation: Kobe
  • Year and Date: 2008-12-11
• [Presentation] Detection and diagnostics of amyloidic proteins using luminescent conjugated polymers (2008)
  • Author(s): Zako, T.
  • Organizer: Joint Meeting of the Japanese Biochemical Society and the Molecular Biology Society of Japan
  • Place of Presentation: Kobe, Japan
  • Year and Date: 2008-12-11
• [Presentation] Development of PEGylated upconversion nanophosphors as a fluorescence bioimaging probe and its application for tumor cell imaging (2008)
  • Author(s): Tamotsu Zako, Hiroyasu Nagata, Naofumi Terada, Arata Utsumi, Masafumi Sakono, Hiroshi Ueda, Masafumi Yohda, Kohei Soga and Mizuo Maeda
  • Organizer: YABEC2008
  • Place of Presentation: Tokyo
  • Year and Date: 2008-11-30
• [Presentation] Development of PEGylated upconversion nanophosphors as a fluorescence bioimaging probe and its application for tumor cell imaging (2008)
  • Author(s): Zako, T.
  • Organizer: YABEC2008
  • Place of Presentation: Tokyo, Japan
  • Year and Date: 2008-11-30
• [Presentation] Small heat shock protein (sHsp)はストレス条件下においてアミロイドβと複合化し無毒のアミロイド様繊維を生成する (2008)
  • Author(s): 座古保、迫野昌文、内海現太、養王田正文、前田瑞夫
  • Organizer: 第3回臨床ストレス応答学会
  • Place of Presentation: 秋田
  • Year and Date: 2008-11-15
• [Presentation] Small heat shock protein (sHsp)はストレス条件下においてアミロイド6と複合化し無毒のアミロイド様繊維を生成する (2008)
  • Author(s): 座古保
  • Organizer: 第3回臨床ストレス応答学会
  • Place of Presentation: 秋田
  • Year and Date: 2008-11-15
• [Presentation] 分子シャペロンによる新規なアミロイド形成 (2008)
  • Author(s): 座古保、迫野昌文、内海現太、養王田正文、前田瑞夫
  • Organizer: 化学工学会沖縄大会
  • Place of Presentation: 沖縄
  • Year and Date: 2008-08-08
• [Presentation] Prefoldin prudentially interacts with group II chaperonin in its ADP bound open conformation (2008)
  • Author(s): Tamotsu Zako, Yosuke Murase, Ryo Iizuka, Taro Kanzaki, Masafumi Shimizu, Masafumi Yohda and Mizuo Maeda
  • Organizer: 33^<rd> FEBS congress and 11^<th> IUBMB conference
  • Place of Presentation: Athens, Greece
  • Year and Date: 2008-06-30
• [Presentation] Formation of Novel Non-toxic Insulin Amyloid Filaments by reducing agent (2008)
  • Author(s): Tamotsu Zako, Masafumi Sakono, Naomi Hashimoto, Masaki Ihara and Mizuo Maeda
  • Organizer: 8^<th> World Biomaterial Congress
  • Place of Presentation: Amsterdam, Netherlands
  • Year and Date: 2008-05-29
• [Presentation] Formation of highly toxic soluble amyloid beta oligomers by the molecular chaperone prefoldin (2008)
  • Author(s): Masafumi Sakono, Tamotsu Zako, Hiroshi Ueda, Masafumi Yohda and Mizuo Maeda
  • Organizer: Cold Spring Harbor Meeting Molecular Chaperons & Stress responses
  • Place of Presentation: New York, USA
  • Year and Date: 2008-05-05
• [Presentation] Interaction of prefoldin with group II chaperonin in the presence of various nucleotides (2007)
  • Author(s): Tamotsu Zako, Yosuke Murase, Andrei Zhukov, Robert Karlsson, Masafumi Shimizu, Ryo Iizuka, Mizuo Maeda, and Masafumi Yohda
  • Organizer: 2^<nd> World conference of Stress
  • Place of Presentation: Budapest, Hunagry
  • Year and Date: 2007-08-25
• [Presentation] Interaction of prefoldin with group II chaperonin in the presence of various nucleotides (2007)
  • Author(s): Tamotsu Zako
  • Organizer: 2nd World conference of Stress
  • Place of Presentation: Budapest, Hungary
  • Year and Date: 2007-08-25
• [Presentation] プレフォルディン-シャペロニンの相互作用解析による基質受け渡し機構の解明 (2007)
  • Author(s): 座古保, 村瀬陽介, 清水雅史, 飯塚怜, 前田瑞夫, 養王田正文
  • Organizer: 蛋白質科学会年会
  • Place of Presentation: 仙台
  • Year and Date: 2007-05-25
• [Presentation] プレフォルディンーシャペロニンの相互作用解析による基質受け渡し機構の解明 (2007)
  • Author(s): 座古保
  • Organizer: 蛋白質科学会年会
  • Place of Presentation: 仙台
  • Year and Date: 2007-05-25
• [Book] "Chaperonin and prefoldin - Two molecular chaperones that work cooperatively in archaea and eukaryote" (Invited Review) in Heat shock proteins : New Research (Edited by Emma Moreland and Camille Vincent) (2008)
  • Author(s): Zako, T., Iizuka, R., Kanzaki, T., Maeda, M. and Yohda, M.
  • Publisher: Nova Science Publishers, Inc., New York, USA
• [Book] Heat shock proteins : New Research Chapter XXI (2008)
  • Author(s): Zako, T.
  • Total Pages: 23
  • Publisher: Nova Science Publishers Inc., New York, USA