Elucidating the mechanism for diffusion of microtubule-binding proteins using charged nanoparticles.
| Project/Area Number |
19770138
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Biophysics
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| Research Institution | The Institute of Physical and Chemical Research |
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Principal Investigator |
MINOURA Itsushi The Institute of Physical and Chemical Research, 分子動態解析技術開発チーム, 研究員 (70373371)
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| Project Period (FY) |
2007 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥600,000)
Fiscal Year 2009: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2008: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2007: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Keywords | キネシン / 微小管 / モーター蛋白質 / 弱結合 / ミカエリスメンテンモデル / 非特異的静電相互作用 / ブラウン運動 / ナノ粒子 / KIF1A / チューブリン / ミカエリスーメンテン / 静電ポテンシャル / 1次元拡散運動 / 静電相互作用 |
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| Research Abstract |
Several types of motor proteins such as KIF1A, MCAK, and dynein are known to show diffusional motion along microtubules. To elucidate the role of electrostatic interaction on this motion, I measured the interaction between positively charged nanoparticles of 60 nm in diameter and microtubules. The particles interact with microtubules in a charge-dependent manner and showed one-dimensional diffusion along microtubules. The motion was analyzed using a Michaelis-Menten type kinetic model. The result revealed that there are two-states : "diffusion"and "binding", during the diffusional motion.
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Report
(4 results)
Research Products
(17 results)
