Elucidating the mechanism for diffusion of microtubule-binding proteins using charged nanoparticles.

• Project/Area Number: 19770138
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Biophysics
• Research Institution: The Institute of Physical and Chemical Research
• Principal Investigator: MINOURA Itsushi The Institute of Physical and Chemical Research, 分子動態解析技術開発チーム, 研究員 (70373371)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥600,000); Fiscal Year 2009: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2008: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2007: ¥1,300,000 (Direct Cost: ¥1,300,000)
• Keywords: キネシン / 微小管 / モーター蛋白質 / 弱結合 / ミカエリスメンテンモデル / 非特異的静電相互作用 / ブラウン運動 / ナノ粒子 / KIF1A / チューブリン / ミカエリスーメンテン / 静電ポテンシャル / 1次元拡散運動 / 静電相互作用

Research Abstract

Several types of motor proteins such as KIF1A, MCAK, and dynein are known to show diffusional motion along microtubules. To elucidate the role of electrostatic interaction on this motion, I measured the interaction between positively charged nanoparticles of 60 nm in diameter and microtubules. The particles interact with microtubules in a charge-dependent manner and showed one-dimensional diffusion along microtubules. The motion was analyzed using a Michaelis-Menten type kinetic model. The result revealed that there are two-states : "diffusion"and "binding", during the diffusional motion.

Research Products

• [Journal Article] One-dimensional Brownian motion of charged nanoparticles along microtubules: A model system for weak binding interactions. (2010)
  • Author(s): Itsushi Minoura, Eisaku Katayama, Ken Sekimoto, Etsuko Muto
  • Journal Title: Biophysical Journal 98(8) Pages: 1589-1597
  • Peer Reviewed
• [Journal Article] One-dimensional Brownian motion of charged nanoparticles along microtubules : A model system for weak binding interactions (2010)
  • Author(s): Itsushi Minoura, Eisaku Katayama, Ken Sekimoto, Etsuko Muto
  • Journal Title: Biophysical Journal 98(8)(in press)
  • Peer Reviewed
• [Presentation] C-terminal tail of tubulin nonessential for Brownian motion of KIFlA (2010)
  • Author(s): 出川雅士, 箕浦逸史, 内村誠一, 武藤悦子
  • Organizer: 54th Annual Meeting of Biophysical Society
  • Place of Presentation: San Francisco(アメリカ合衆国)
  • Year and Date: 2010-02-24
• [Presentation] Three-state model for one-dimensional Brownian motion of charged nanoparticles along microtubules (2010)
  • Author(s): Itsushi Minoura, Eisaku Katayama, Ken Sekimoto, Seiichi Uchimura, Masashi Degawa, Etsuko Muto
  • Organizer: 54th Annual Meeting of Biophysical Society
  • Place of Presentation: San Francisco(アメリカ合衆国)
  • Year and Date: 2010-02-21
• [Presentation] Three-state model for one-dimensional Brownian motion of charged nanoparticles along microtubules. (2010)
  • Author(s): Itsushi Minoura, E. Katayama, K. Sekimoto, S. Uchimura, M. Degawa, E. Muto
  • Organizer: 54th Annual Meeting of Biophysical Society
  • Place of Presentation: サンフランシスコ
• [Presentation] C-terminal tail of tubulin nonessential for Brownian motion of KIF1A (2010)
  • Author(s): Masashi Degawa, Itsushi Minoura, S. Uchimura, E. Muto
  • Organizer: 54th Annual Meeting of Biophysical Society
  • Place of Presentation: サンフランシスコ
• [Presentation] The role of extended C-terminal tail of tubulin on weak-binding interaction between motor proteins and microtubules(チューブリンC末端の伸長した構造が弱結合に果たす役割) (2009)
  • Author(s): 箕浦逸史、内村誠一、出川雅士、武藤悦子
  • Organizer: 第47回日本生物物理学会年会
  • Place of Presentation: 徳島市
  • Year and Date: 2009-10-31
• [Presentation] Is the tubulin C-terminus tail necessary for the 1D Brownian motion of KIFlA?(KIFlAの一次元ブラウン運動にチューブリンC末端は必要か?) (2009)
  • Author(s): 出川雅士、箕浦逸史、内村誠一、武藤悦子
  • Organizer: 第47回日本生物物理学会年会
  • Place of Presentation: 徳島市
  • Year and Date: 2009-10-31
• [Presentation] The role of extended C-terminal tail of tubulin on weak-binding interaction between motor proteins and microtubules(チューブリンC末端の伸長した構造が弱結合に果たす役割) (2009)
  • Author(s): Itsushi Minoura, Seiichi Uchimura, Masashi Degawa, Etsuko Muto
  • Organizer: 47th Annual Meeting of the Biophysical Society of Japan(第47回日本生物物理学会年会)
  • Place of Presentation: 徳島
• [Presentation] Is the tubulin C-terminus tail necessary for the 1D Brownian motion of KIF1A? (KIF1Aの一次元ブラウン運動にチューブリンC末端は必要か?) (2009)
  • Author(s): Masashi Degawa, Itsushi Minoura, Seiichi Uchimura, Etsuko Muto
  • Organizer: 47th Annual Meeting of the Biophysical Society of Japan(第47回日本生物物理学会年会)
  • Place of Presentation: 徳島
• [Presentation] 荷電粒子の1次元ブラウン運動は微小管の電荷分布にどのように影響されるか? (2008)
  • Author(s): 箕浦逸史, 内村誠一, 武藤悦子
  • Organizer: 日本生物物理学会第46会年会
  • Place of Presentation: 福岡
  • Year and Date: 2008-12-04
• [Presentation] 微小管表面の静電ポテンシャルを反映した荷電粒子の1次元ブラウン運動 (2008)
  • Author(s): 箕浦逸史、武藤悦子
  • Organizer: ナノ学会第6回大会
  • Place of Presentation: 福岡
• [Presentation] How does the charge distribution of microtubules affect the one-dimensional Brownian motion of charged particles? (荷電粒子の1次元ブラウン運動は微小管の電荷分布にどのように影響されるか?) (2008)
  • Author(s): Itsushi Minoura, Seiichi Uchimura, Etsuko Muto
  • Organizer: 46th Annual Meeting of the Biophysical Society of Japan(日本生物物理学会第46回年会)
  • Place of Presentation: 福岡
• [Remarks]
  • URL: http://mtu.brain.riken.jp/research.html
• [Remarks]
  • URL: http://researchmap.jp/minoura124/
• [Remarks]
  • URL: http://mtu.brain.riken.jp/
• [Remarks]
  • URL: http://mtu.brain.riken.jp/Brownianmotion_Jap_notyet.html