Analysis of Transcriptional regulation by HSFs in development process

• Project/Area Number: 19770148
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Molecular biology
• Research Institution: Yamaguchi University
• Principal Investigator: FUJIMOTO Mitsuaki Yamaguchi University, 大学院・医学系研究科, 講師 (80359900)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,720,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥420,000); Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2007: ¥1,900,000 (Direct Cost: ¥1,900,000)
• Keywords: 熱ショック転写因子 / レンズ / 発生過程 / 転写

Research Abstract

レンズにおいて熱ショック転写因子HSF4の結合領域を網羅的に調べた結果、HSF4結合領域のほとんどはイントロンあるいは近傍の遺伝子から10 kb 以上離れた領域に存在した.この領域は、HSF群の結合が発生過程で変化し、近傍に存在する遺伝子は熱ショックによって誘導されることが分かった.また、HSF4の結合が周辺部のクロマチン修飾に影響を及ぼすことも明らかになった.

Research Products

• [Journal Article] Analysis of HSF4 Binding Regions Reveals Its Necessity For GeneRegulation during Development and Heat Shock Response in Mouse Lenses (2008)
  • Author(s): MITSUAKI FUJIMOTO, KOJI OSHIMA, TOYOHIDE SHINKAWA, BEI BEI WANG, SACHIYE INOUYE, NAOKI HAYASHIDA, RYOSUKE TAKII, AKIRA NAKAI
  • Journal Title: The Journal of biological Chemistry 30 Pages: 29961-29970
  • Peer Reviewed
• [Journal Article] Attenuation of Progressive Hearing Loss ina Model ofAge-related Hearing Loss by a Heat Shock Proteins Inducer (2008)
  • Author(s): TAKEFUMI MIKURIYA, KAZUMA SUGAHARA,KAZUTAKA SUGIMOTO, MITSUAKI FUJIMOTO,TSUYOSHI TAKEMOTO, MAKOTO HASHIMOTO,YOSHINOBU HIROSE, HIROAKI SHIMOGORI,NAOKI HAYASHIDA, SACHIYE INOUYE, NAKAIAKIRA, HIROSHI YAMASHITA
  • Journal Title: Geranylgeranylactone, Brain Research 1212 Pages: 9-17
  • Peer Reviewed
• [Journal Article] Analysis of HSF 4 binding regions reveals its necessity for gene regulation during development and heat shock response in mouse lenses (2008)
  • Author(s): Mitsuaki Fujimoto
  • Journal Title: The Journal of biological Chemistry 283 Pages: 29961-29970
  • Peer Reviewed
• [Journal Article] Attenuation of progressive hearing loss in a model of age-related hearing loss by a heat shock protein inducer, geranylgeranylacetone (2008)
  • Author(s): Takefumi Mikuriya
  • Journal Title: 30 Pages: 9-17
  • Peer Reviewed
• [Journal Article] Heat shock transcription Factor 1 is required for maintenance of ciliary beating in mice (2007)
  • Author(s): Eiichi Takaki
  • Journal Title: The Journal of biological Chemistry 282 Pages: 37285-37292
  • Peer Reviewed
• [Journal Article] Heat shock transcription Factor 1 opens chromatin structure of interleukin-6 Promoter to facilitate binding of an activator or a repressor (2007)
  • Author(s): Sachiye Inouye
  • Journal Title: The Journal of biological Chemistry 282 Pages: 33210-33271
  • Peer Reviewed
• [Journal Article] Genetic evidence for a protective role for heat shock factor 1 and heat shock protein 70 against colitis (2007)
  • Author(s): Ken-Ichiro Tanaka
  • Journal Title: The Journal of biological Chemistry 282 Pages: 23240-23252
  • Peer Reviewed
• [Presentation] 熱ショック転写因子HSF4の発生過程と熱ショック応答での役割 (2008)
  • Author(s): 藤本充章
  • Organizer: 日本分子生物学会・日本生化学学会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-10
• [Presentation] 熱ショック転写因子HSF4が認識するDNA結合配列の同定 (2007)
  • Author(s): 藤本 充章
  • Organizer: 日本分子生物学会・日本生化学学会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-12
• [Remarks]
  • URL: http://ds22.cc.yamaguchi-u.ac.jp/~seika2/