Identification of activators for a functional DNA sequence that promotes replication initiation.

• Project/Area Number: 19770150
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Molecular biology
• Research Institution: Kyushu University
• Principal Investigator: FUJIMITSU Kazuyuki Kyushu University, 大学院・薬学研究院, 特任助教 (90448431)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥3,940,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥540,000); Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2008: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2007: ¥1,600,000 (Direct Cost: ¥1,600,000)
• Keywords: 核酸 / 遺伝子 / ゲノムDNA複製 / 蛋白質 / 活性制御 / 機能性DNA / 機能性 DNA

Research Abstract

To study the mechanism for regulation of the initiation of DNA replication, I focused on a specific DNA segment that activates the initiator of E. coli chromosomal replication, DnaA. Our recent results suggested the presence of a soluble factor that can promote the activity of the DNA segment. Here, we reveal that two DNA binding proteins simultaneously function to stimulate the DNA segment in vitro and in vivo.

Research Products

• [Journal Article] Regulation of the replication cycle: conserved and diverse regulatory systems for DnaA and oriC (2010)
  • Author(s): Katayama, T., Ozaki, S., Keyamura, K., Fujimitsu, K
  • Journal Title: Nature Reviews Microbiology 8巻 Pages: 163-170
  • Peer Reviewed
• [Journal Article] Regulation of the replication cycle : conserved and diverse regulatory systems for DnaA and oriC (2010)
  • Author(s): Katayama, T., et al
  • Journal Title: Nature Reviews Microbiology 8 Pages: 163-170
  • Peer Reviewed
• [Journal Article] Specific genomic sequences of E. coli promote replicational initiation by directly reactivating ADP-DnaA (2009)
  • Author(s): Fujimitsu, K., Senriuchi, T., Katayama T
  • Journal Title: Genes & Development 23巻 Pages: 1221-1233
  • Peer Reviewed
• [Journal Article] Specific genomic sequences of E.coli promote replicational initiation by directly reactivating ADP-DnaA (2009)
  • Author(s): Fujimitsu, K., et al
  • Journal Title: Genes & Development 23 Pages: 1221-1233
  • Peer Reviewed
• [Journal Article] Loss of Hda activity stimulates replication initiation from I-box, but not R4 mutant origins in Escherichia coli (2008)
  • Author(s): Riber, L., Fujimitsu, K., Katayama, T., Lobner-Olesen, A
  • Journal Title: Mol Microbiol 71巻 Pages: 107-122
  • Peer Reviewed
• [Journal Article] Modes of overinitiation, dnaA gene expression, and inhibition of cell division in a novel cold-sensitive hda mutant of Escherichia coli (2008)
  • Author(s): Fujimitsu, K., Su'etsugu, M., Yamaguchi, Y., Mazda, K., Fu, N., Kawakami, H., Katayama, T.
  • Journal Title: J Bacteriol 190巻 Pages: 5368-5381
  • Peer Reviewed
• [Journal Article] Modes of overinitiation, dnaA gene expression, and inhibition of cell division in a novel coldsensitive hda mutant of Escherichia coli (2008)
  • Author(s): Fujimitsu, K., et.al.
  • Journal Title: J Bacteriol 190 Pages: 5368-5381
  • Peer Reviewed
• [Journal Article] Loss of Hda activity stimulates replication initiation from I-box, but not R4 mutant origins in Escherichia coli (2008)
  • Author(s): Riber, L., et.al.
  • Journal Title: Mol Microbiol 71 Pages: 107-122
  • Peer Reviewed
• [Journal Article] The interaction of DiaA and DnaA regulates the replication cycle in E. coli by directly promoting ATP DnaA-specific initiation complexes (2007)
  • Author(s): Keyamura, K., Fujikawa, N., Ishida, T., Ozaki, S., Su'etsugu, M., Fujimitsu, K., Kagawa, W., Yokoyama, S., Kurumizaka, H., Katayama, T
  • Journal Title: Genes & Development 21巻 Pages: 2083-2099
  • Peer Reviewed
• [Journal Article] The interaction of DiaA and DnaA regulates the replication cycle in E. coli by directly promoting ATP DnaA-specific initiation complexes (2007)
  • Author(s): Keyamura, K., et. al.
  • Journal Title: Genes and Development 21 Pages: 2083-2099
  • Peer Reviewed
• [Presentation] 大腸菌染色体複製イニシエーターDnaAは特異的なDNA配列上で高次複合体を形成し活性化される (2009)
  • Author(s): 藤光和之, 他1名
  • Organizer: 第82回生化学会大会
  • Place of Presentation: 兵庫県神戸市
  • Year and Date: 2009-10-24
• [Presentation] 大腸菌染色体複製イニシエーターDnaAは特異的なDNA配列上で高次複合体を形成し活性化される (2009)
  • Author(s): 藤光和之, 片山勉
  • Organizer: 第82回 生化学会大会
  • Place of Presentation: 兵庫県神戸市
  • Year and Date: 2009-10-24
• [Presentation] Multiple copies of the ribonucleotide reductase genes repress overinitiation of chromosomal replication caused by a novel cold-sensitive hda mutant in Escherichia coli (2008)
  • Author(s): Fujimitsu, K., 他6名
  • Organizer: 3R Symposium 2008
  • Place of Presentation: 静岡県掛川市
  • Year and Date: 2008-10-27
• [Presentation] Multiple copies of the ribonucleotide reductase genes repress overinitiation of chromosomal replication caused by a novel coldsensitive hda mutant in Escherichia coli (2008)
  • Author(s): Fujimitsu, K., et.al.
  • Organizer: 3R Symposium 2008
  • Place of Presentation: 静岡県掛川市
  • Year and Date: 2008-10-27
• [Presentation] Specific E. coli chromosomal regions can reactivate replication initiation activity of DnaA by exchanging bound nucleotide from ADP to ATP in vitro (2008)
  • Author(s): Fujimitsu, K, 他2名
  • Organizer: EMBO workshop (Replication & Segregation of Chromosomes)
  • Place of Presentation: ノルウェー王国ヤイロ
  • Year and Date: 2008-06-16
• [Presentation] Specific E. coli chromosomal regions can promote exchanging of DnaA-bound nucleotide from ADP to ATP (2008)
  • Author(s): Fujimitsu, K, 他2名
  • Organizer: Keystone symposia(DNA replication and recombination)
  • Place of Presentation: アメリカ合衆国ニューメキシコ州サンタフェ
  • Year and Date: 2008-02-11
• [Presentation] Specific E. coli chromosomal regions can promote exchanging of DnaA-bound nucleotide from ADP to ATP. (2008)
  • Author(s): Fujimitsu, K, et. al.
  • Organizer: Keystone symposia(DNA replication and recombinatin)
  • Place of Presentation: アメリカ合衆国ニューメキシコ州サンタフェ
  • Year and Date: 2008-02-11
• [Presentation] 複製開始蛋白質DnaAを再活性化する2種類の機能性DNA部位は異なる制御様式をもつ (2007)
  • Author(s): 藤光和之, 他2名
  • Organizer: 第4回21世紀大腸菌研究会
  • Place of Presentation: 静岡県藤枝市
  • Year and Date: 2007-07-17