Protein complex analysis for the Chromatin regulation and its functional network

• Project/Area Number: 19770152
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Molecular biology
• Research Institution: Nagoya City University
• Principal Investigator: TAGAMI Hideaki Nagoya City University, 大学院・システム自然科学研究科, 准教授 (70273216)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,790,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥390,000); Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2007: ¥2,100,000 (Direct Cost: ¥2,100,000)
• Keywords: ヒストン / タンパク質複合体 / クロマチン制御 / 機能ネットワーク / ヒストン複合体

Research Abstract

本研究ではヒストン複合体解析を通してクロマチン形成の分子機構、および動的なクロマチン制御を介する細胞機能の解明を目指した。酵母をモデル系として、様々な条件下におけるヒストン複合体を精製し、ヒストン相互作用因子ネットワークとそのダイナミクス解析を行った。酵母やヒトのヒストン複合体を比較することでクロマチン形成の共通の分子基盤と戦略のバリエーションが示唆された。また、ヒストンシャペロンFACTが複製や転写だけでなく、よりグローバルにヒストンH2A/H2Bをクロマチンから解離させるという興味深い結果を得た。

Research Products

• [Journal Article] The HP1α-CAF-1-SetDB1 complex provides H3K9me1 for Suv39-mediated K9me3 in pericentric heterochromatin (2009)
  • Author(s): Alejandra Loyola, Hideaki Tagami, Tiziana Bonaldi, Daniele Roche, Jean Pierre Quivy, Axel Imhof, Yoshihiro Nakatani, Sharon YR Dent, and Genevieve Almouzni
  • Journal Title: EMBO Reports (accepted)
  • Peer Reviewed
• [Journal Article] HJURP, a key CENP-A-partner for maintenance and deposition of CENP-A at centromeres at late telophase/G1 (2009)
  • Author(s): Elaine M. Dunleavy, Daniele Roche, Hideaki Tagami, Nicolas Lacoste, Dominique Ray-Gallet, Yusuke Nakamura, Yataro Daigo, Yoshihiro Nakatani, Genevieve Almouzni-Pettinotti.
  • Journal Title: Cell 137 Pages: 487-497
  • Peer Reviewed
• [Journal Article] Mechanism and significance of specific proteolytic cleavage of Reelin (2009)
  • Author(s): Shiori Kohno, Takao Kohno, Yoshimi Nakano, Kenta Suzuki, Moe Ishii, Hideaki Tagami, Atsushi Baba and Mitsuharu Hattori
  • Journal Title: Biochemical and Biophysical Research Communications 380 Pages: 93-97
  • Peer Reviewed
• [Journal Article] Mechanism and significance of specific proteolytic cleavage of Reelin (2009)
  • Author(s): Shiori Kohno, et.al.
  • Journal Title: Biochem Biophys Res Commun 380 Pages: 93-97
  • Peer Reviewed
• [Journal Article] Chk1 is a histone H3 threonine 11 kinase that regulates DNA damage-induced transcriptional repression (2008)
  • Author(s): Midori Shimada, Hiroyuki Niida, Doaa H. Zineldeen, Hideaki Tagami, Masafumi Tanaka, Hiroyuki Saito, and Makoto Nakanishi
  • Journal Title: Cell 132 Pages: 221-232
  • Peer Reviewed
• [Journal Article] Chkl is a histone H3 threonine 11 kinase that regulates DNA damage-induced transcriptional repression (2008)
  • Author(s): Shimada, M., et. al.
  • Journal Title: Cell 132 Pages: 221-232
  • Peer Reviewed
• [Journal Article] An oncoprotein from the plant pathogen Agrobacterium has histone-chaperone-like activity (2007)
  • Author(s): Shinji Terakura, Yoshihisa Ueno, Hideaki Tagami, Saeko Kitakura, Chiyoko Machida, Hiroetsu Wabiko, Hiroji Aiba, Leon Otten, Hironaka Tsukagoshi, Kenzo Nakamura, and Yasunori Machida
  • Journal Title: Plant Cell 19 Pages: 2855-2865
  • Peer Reviewed
• [Journal Article] An oncoprotein from the plant pathogen Agrobacterium has histone-chaperone-like activity (2007)
  • Author(s): Terakura, S., et. al.
  • Journal Title: Plant Cell 19 Pages: 2855-2865
  • Peer Reviewed
• [Presentation] 新規クロマチン因子Mlo2とヒストンH3/H4相互作用 (2009)
  • Author(s): 田上英明
  • Organizer: 遺伝研研究会「単細胞における複合システム系の連帯と統合」
  • Place of Presentation: 三島
  • Year and Date: 2009-03-24
• [Presentation] 新規クロマチン関連因子Mlo2とヒストンH3/H4相互作用 (2009)
  • Author(s): 保坂いづみ、田上英明
  • Organizer: 第26回染色体ワークショップ
  • Place of Presentation: 姫路
  • Year and Date: 2009-01-27
• [Presentation] FACTは出芽酵母ヒストンH2A/H2Bダイナミクスを規定する (2009)
  • Author(s): 田上英明、加藤麻希
  • Organizer: 第26回染色体ワークショップ
  • Place of Presentation: 姫路
  • Year and Date: 2009-01-26
• [Presentation] FACTを介した酵母ヒストンH2A/H2Bダイナミクス (2008)
  • Author(s): 田上英明
  • Organizer: BMB2008
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-10
• [Presentation] 出芽酵母H2A-H2B複合体の分子解剖 (2008)
  • Author(s): 加藤麻希、田上英明
  • Organizer: BMB2008
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-10
• [Presentation] EACTを介した酵母ヒストンH2A/H2Bダイナミクス (2008)
  • Author(s): 田上英明、加藤麻希
  • Organizer: BMB2008
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-10
• [Presentation] 酵母ヒストンH2A/H2Bから見るクロマチンダイナミクス (2008)
  • Author(s): 田上英明
  • Organizer: クロマチン研究会
  • Place of Presentation: 三島
  • Year and Date: 2008-10-22
• [Presentation] FACT MEDIATES HISTONE H2A/H2B DYNAMICS IN YEAST (2008)
  • Author(s): Hideaki Tagami and Maki Kato
  • Organizer: International Symposium on Chromosome Dynamics in Ise
  • Place of Presentation: 三重
  • Year and Date: 2008-05-28
• [Book] 「S1マッピングによるRNA末端の決定」『RNA実験ノート』上無敵のバイオテクニカルシリーズ 稲田利文、塩見春彦 編 (2008)
  • Author(s): 田上英明
  • Publisher: 羊土社