Analysis of the proteaosme assembly pathway

• Project/Area Number: 19770156
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Molecular biology
• Research Institution: The University of Tokyo (2008); Tokyo Metropolitan Organization for Medical Research (2007)
• Principal Investigator: YASHIRODA Hideki The University of Tokyo, 大学院薬学系研究科, 准教授 (20311425)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,490,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥390,000); Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2007: ¥1,800,000 (Direct Cost: ¥1,800,000)
• Keywords: 生体高分子構造・機能 / プロテアソーム / シャペロン / 出芽酵母 / X線結構造解析

Research Abstract

出芽酵母を用いた遺伝学的解析により、プロテアソームによるタンパク質分解に異常を来す変異株を取得し、責任遺伝子を同定しDMP1, DMP2 (degradation of misfolded protein)と命名した。Dmp1 とDmp2 はヘテロ二量体を形成し、形成途上のプロテアソームの20S 複合体と一時的に会合することを明らかにした。DMP1,DMP2 欠損株では20S 複合体の形成が障害され、特に異常なαリングの形成が生じることから、正しいαリングの形成を支援することによりプロテアソームの形成を助けるシャペロン分子であることを明らかにした。

Research Products

• [Journal Article] Molecular mechanisms of proteasome assembly (2009)
  • Author(s): Murata S, Yashiroda H, Tanaka K
  • Journal Title: Nat Rev Mol Cell Biol 10(2) Pages: 104-15
  • Peer Reviewed
• [Journal Article] Molecular mechanisms of proteasome assembly (2009)
  • Author(s): Shigeo Murata
  • Journal Title: Nature Reviews Molecular Cell Biology 10 Pages: 104-15
  • Peer Reviewed
• [Journal Article] Dissecting β-ring assembly pathway of the mammalian 20S proteasome (2008)
  • Author(s): Hirano Y, Kaneko T, Okamoto K, Bai M, Yashiroda H, Furuyama K, Kato K, Tanaka K, Murata S
  • Journal Title: EMBO J 27(16) Pages: 2204-13
  • Peer Reviewed
• [Journal Article] Crystal structure of a chaperone complex that contributes to the assembly of yeast 20S proteasomes (2008)
  • Author(s): Yashiroda H, Mizushima T, Okamoto K, Kameyama T, Hayashi H, Kishimoto T, Niwa S, Kasahara M, Kurimoto E, Sakata E, Takagi K, Suzuki A, Hirano Y, Murata S, Kato K, Yamane T, Tanaka K
  • Journal Title: Nat Struct Mol Biol 15(3) Pages: 228-36
  • Peer Reviewed
• [Journal Article] 出芽酵母20S プロテアソーム形成に関わるシャペロン複合体dmp1/Dmp2の立体構造解析 (2008)
  • Author(s): 八代田英樹、水島恒裕
  • Journal Title: 細胞工学, (秀潤社) 27 Pages: 474-475
• [Journal Article] プロテアソームの分子集合機構 (2008)
  • Author(s): 平野祐子、八代田英樹、佐伯泰
  • Journal Title: 実験医学, (羊土社) 26(2) Pages: 102-109
• [Journal Article] Dissecting beta-ring assembly pathway of the mammalian 20S proteasome (2008)
  • Author(s): Yuko Hirano
  • Journal Title: The EMBO Journal 27 Pages: 2204-13
  • Peer Reviewed
• [Journal Article] Crystal structure of a chaperone complex that contributes to the assembly of yeast 20S proteasomes (2008)
  • Author(s): 八代田 英樹
  • Journal Title: Nature Structural & Molecular Biology 15 Pages: 228-236
  • Peer Reviewed
• [Presentation] アミノ酸アナログによるストレス応答に必要なプロテアソーム会合支援複合体Dmp1-Dmp2 (2008)
  • Author(s): 八代田英樹
  • Organizer: BMB2008
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-11
• [Presentation] アミノ酸アナログによるストレス応答に必要なプロテアソーム会合支援複合体Dmp1-Dmp2 (2008)
  • Author(s): 八代田英樹
  • Organizer: BMB2008
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2008-12-11
• [Presentation] The Dmp1-Dmp2 complex helps to assemble the yeast 20S proteasome (2008)
  • Author(s): Hideki Yashiroda
  • Organizer: FASEB Summer Research conference
  • Place of Presentation: Vermont, USA
  • Year and Date: 2008-12-11
• [Presentation] The Dmp1-Dmp2 complex helps to assemble the yeast 20S proteasome (2008)
  • Author(s): Hideki Yashiroda
  • Organizer: 第15回武田科学振興財団生命科学シンポジウム
  • Place of Presentation: シェラトン都ホテル東京
  • Year and Date: 2008-12-02
• [Presentation] The Dmp1-Dmp2 complex helps to assemble yeast 20S proteasomes (2008)
  • Author(s): Hideki Yashiroda
  • Organizer: Zomes V
  • Place of Presentation: RIKEN Yokohama
  • Year and Date: 2008-11-12
• [Presentation] 20Sプロテアソームの形成を支援する出芽酵母Dmp1-Dmp2の解析 (2008)
  • Author(s): 八代田英樹
  • Organizer: 東京大学生命科学研究ネットワークシンポジウム2008
  • Place of Presentation: 東京大学安田講堂
  • Year and Date: 2008-09-23
• [Presentation] The Dmp1-Dmp2 complex helps to assemble the yeast 20S proteasome (2008)
  • Author(s): Hideki Yashiroda
  • Organizer: FASEB summer research conferences
  • Place of Presentation: Vermont
• [Presentation] 出芽酵母20S プロテアソーム形成に関わる新規シャペロン複合体 (2007)
  • Author(s): 八代田英樹
  • Organizer: BMB2007
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-14
• [Presentation] Identification and characterization of novel proteasome associated proteins in S.cerevisiae (2007)
  • Author(s): 八代田 英樹
  • Organizer: The Ubiquitin Family
  • Place of Presentation: Cold Siring Harbor Laboratory, USA
• [Presentation] 20Sプロテアソーム会合支援複合体Dmpl/Dmp2の解析 (2007)
  • Author(s): 八代田 英樹
  • Organizer: 第40回酵母遺伝学フォーラム
  • Place of Presentation: 大阪
• [Presentation] 出芽酵母20Sプロテアソーム形成に関わる新規シャペロン複合体 (2007)
  • Author(s): 八代田 英樹
  • Organizer: BMB2007
  • Place of Presentation: 横浜
• [Remarks]
  • URL: http://www.f.u-tokyo.ac.jp/?tanpaku/
• [Remarks]
  • URL: http://www.f.u-tokyo.acjp/tanpaku/
• [Remarks]
  • URL: http://www.rinshoken.or.jp/MO/index-jp.htm