Identification and structure-function analyses of recognition/processing factors for arrested replication forks

• Project/Area Number: 19770157
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Molecular biology
• Research Institution: Tokyo Metropolitan Organization for Medical Research
• Principal Investigator: TANAKA Taku Tokyo Metropolitan Organization for Medical Research, 東京都臨床医学総合研究所, 主任研究員 (80425686)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,880,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥480,000); Fiscal Year 2008: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2007: ¥1,800,000 (Direct Cost: ¥1,800,000)
• Keywords: DNA複製 / 複製再開始 / ・複製フォーク / 分子認識 / 分子間相互作用 / タンパク質 / マイクロアレイ / 核酸 / 結晶構造解析 / 大腸菌 / 複製

Research Abstract

停止フォークセンサー、大腸菌PriAタンパク質の DNA結合ドメインの結晶構造を解析し、極めてユニークなフォーク特異的認識機構を有していることを明らかにした。また、真核細胞のフォーク安定化因子、Mrc1、Swi1/3タンパク質が、直接物理的に結合しており、停止フォーク上で協調した相互作用をすることを見出した。これらの結果は、真核細胞のチェックポイント機構解明に重要な情報を提供するものである。

Research Products

• [Journal Article] Structural basis of the 3'-end recognition of a leading strand in stalled replication forks by PriA (2007)
  • Author(s): Sasaki, K., Ose, T., Okamoto, N., Maenaka, K., Tanaka, T., Masai, H., Saito, M., Shirai, T., and Kohda, D.
  • Journal Title: EMBO J 28 Pages: 2584-2593
  • Peer Reviewed
• [Journal Article] Escherichia coli PriA protein, two modes of DNA binding and activation of ATP hydrolysis (2007)
  • Author(s): Tanaka, T., Mizukoshi, T., Sasaki, K., Kohda, D., and Masai, H
  • Journal Title: J. Biol. Chem 282 Pages: 19917-19927
  • Peer Reviewed
• [Journal Article] Strustural basis the 3'-end recognition of a leading strand in stall replication forks by PriA (2007)
  • Author(s): Sasaki, K., Ose T., Okamoto n., Maenaka K., Tanaka T., Masai H ., Saito M., Shirai T., and Kohda D.
  • Journal Title: EMBO Journal 26 Pages: 2589-2593
  • Peer Reviewed
• [Journal Article] Escherishia PriA protein, two modes of DNA binding and activation of ATP hydrolysis (2007)
  • Author(s): Tanaka, T., Mizukohsi, T., Sasaki, K., Kohda, D., and Masai, H.
  • Journal Title: Journal of Biological Chemistry 282 Pages: 19917-19927
  • Peer Reviewed
• [Presentation] Regulation of replication fork integrity during S phase. (2008)
  • Author(s): Hisao Masai, Motoshi Hayano, Seiji Matsranoto, Yutaka Kanoh, Mika Yokoyama, Michie Shimmoto, Naoko Kakusho, Naoko Yoshizawa-Sugata and Taku Tanaka.
  • Organizer: 第31回 日本分子生物学会
  • Place of Presentation: 兵庫県神戸市
  • Year and Date: 2008-12-10
• [Presentation] Regulation of replication fork in maintenance of genomic integrity. (2008)
  • Author(s): Hisao Masai, Motoshi Hayano, Seiji Matsumoto, Yutaka Kanoh, Michie Shimmoto, Mika Yokoyama, Taku Tanaka, Naoko Kakusho, Naoko Yoshizawa-Sugata, Zhiying You, Sayuri Ito, Chika Taniyama and Ai Ishii.
  • Organizer: 第6回 3R Symposium
  • Place of Presentation: 静岡県掛川市
  • Year and Date: 2008-10-29
• [Presentation] Regulation of assembly and progression of replication fork by Cdc7 kinase. (2008)
  • Author(s): Hisao Masai, Motoshi Hayano, Seiji Matsumoto, Yutaka Kanoh, Mika Yokoyama, Michie Shimmoto, Naoko Kakusho, and Taku Tanaka.
  • Organizer: The First GCEO Symposium at Tokyo Institute of Technology on Chromosome Biology
  • Place of Presentation: 東京
  • Year and Date: 2008-10-24
• [Presentation] Regulation of establishment and maintenance of replication fork by Cdc7 kinase. (2008)
  • Author(s): Hisao Masai, Motoshi Hayano, Seiji Matsumoto, Naoko Kakusho, Mika Yokoyama, Yutaka Kanoh, Michie Simmoto and Taku Tanaka.
  • Organizer: EMBO Conference on "Replication and Segregation of Chromosomes"
  • Place of Presentation: Norway, Geilo
  • Year and Date: 2008-06-19
• [Presentation] Regulation of establishment and maintenance of replication fork by Cdc7 kinase. (2008)
  • Author(s): Hisao Masai, Motoshi Hayano, Seiji Matsumoto, Naoko Kakusho, Mchie Shimmoto, Mika Yokoyama, Yutaka Kanoh, Taku Tanaka and Yiikari Odagiri.
  • Organizer: Ataxia-Telangiectasia Workshop
  • Place of Presentation: 滋賀県大津市
  • Year and Date: 2008-04-25
• [Presentation] 分裂酵母Mrc1タンパクの精製とその性情の解析 (2008)
  • Author(s): 横山 美佳、田中 卓、覺正 直子、正井 久雄
  • Organizer: 第25回染色体ワークショップ
  • Place of Presentation: ポスター発表
• [Presentation] Regulation of establishment and maintenance of replication fork by Cdc7 kinase (2008)
  • Author(s): Hisao Masai, Motoshi Hayano, Seiji Matsumoto, Naoko Kakusho, Michie Shimmoto, Mika Yokoyama, Yutaka Kanoh, Taku Tanaka and Yukari Odagiri
  • Organizer: Ataxia-Telangiectasia Workshop 2008
  • Place of Presentation: 滋賀(招待講演)
• [Presentation] Regulation of establishment and maintenance of replication fork by Cdc7 kinase (2008)
  • Author(s): Hisao Masai, Motoshi Hayano, Seiji Matsumoto, Naoko Kakusho, Mika Yokoyama, Yutaka Kanoh, Michie Simmoto and Taku Tanaka
  • Organizer: EMBO Conference on "Replication and Segregation of Chromosomes"
  • Place of Presentation: ノルウェイ(招待講演)
• [Presentation] Regulation of assembly and progression of replication fork by Cdc7 kinase (2008)
  • Author(s): Hisao Masai, Motoshi Hayano, Seiji Matsumoto, Yutaka Kanoh, Mika Yokoyama, Michie Shimmoto, Naoko Kakusho, and Taku Tanaka
  • Organizer: The First GCEO Symposium at Tokyo Institute of Technology on Chromosome Biology 2008
  • Place of Presentation: 東京(招待講演)
• [Presentation] Regulation of replication fork in maintenance of genomic integrity (2008)
  • Author(s): Hisao Masai, Motoshi Hayano, Seiji Matsumoto, Yutaka Kanoh, Michie Shimmoto, Mika Yokoyama, Taku Tanaka, Naoko Kakusho, Naoko Yoshizawa-Sugata, Zhiying You, Sayuri Ito, Chika Taniyama and Ai Ishii
  • Organizer: 第6回3R Symposium
  • Place of Presentation: 静岡(招待講演)
• [Presentation] Regulation of replication fork integrity during S phase (2008)
  • Author(s): Hisao Masai, Motoshi Hayano, Seiji Matsumoto, Yutaka Kanoh, Mika Yokoyama, Michie Shimmoto, Naoko Kakusho, Naoko Yoshizawa-Sugata and Taku Tanaka
  • Organizer: 第31回日本分子生物学会
  • Place of Presentation: 神戸(招待講演)
• [Presentation] PriAタンパク質:停止複製フォーク特異的認識のための構造基盤 (2007)
  • Author(s): 田中 卓、佐々木 香織、水越 利巳、神田 大輔、正井 久雄
  • Organizer: 第30回日本分子生物学会
  • Place of Presentation: 神奈川県横浜市
  • Year and Date: 2007-12-11
• [Presentation] DNA結合ドメインの2つのモジュールの役割とDNA3'末端結合ポケットの特異な構造 (2007)
  • Author(s): 田中 卓、佐々木 香織、神田 大輔、正井 久雄
  • Organizer: 第4回21世紀大腸菌研究会
  • Place of Presentation: 静岡県藤枝市
  • Year and Date: 2007-07-18
• [Presentation] PriAタンパク質による停止複製フォーク認識:DNA結合ドメインの2つのモジュールの役割とDNA3'末端結合ポケットの特異な構造 (2007)
  • Author(s): 田中 卓、佐々木 香織、神田 大輔、正井 久雄
  • Organizer: 第4回21世紀大腸菌研究会
  • Place of Presentation: 静岡(口頭発表)
• [Presentation] PriAタンパク質:停止複製フォーク特異的認識のための構造基盤 (2007)
  • Author(s): 田中 卓、佐々木 香織、水越 利巳、神田 大輔、正井 久雄
  • Organizer: 第30回日本分子生物学会
  • Place of Presentation: 横浜(口頭およびポスター発表)
• [Presentation] Structural basis for the DNA recognition of PriA protein in the stalled DNA replication fork (2007)
  • Author(s): 佐々木 香織、田中 卓、正井 久雄、前仲 勝実、神田 大輔
  • Organizer: 第30回日本分子生物学会
  • Place of Presentation: 横浜(ポスター発表)
• [Presentation] 、分裂酵母Mrc1タンパクの精製とその性情の解析 (2007)
  • Author(s): 横山 美佳、田中 卓、覺正 直子、正井 久雄
  • Organizer: 第30回日本分子生物学会
  • Place of Presentation: 横浜(ポスター発表)
• [Book] 「分子間相互作用解析ハンドブック」(礒辺俊明,中山敬一,伊藤隆司/編) 第二章 タンパク質-核酸相互作用解析法 1.South-Western法 (2007)
  • Author(s): 田中 卓、正井 久雄
  • Publisher: 羊土社