Investigation of mechanisms for the maintenance of cell polarity in epithelial and neuronal cells

• Project/Area Number: 19770160
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Cell biology
• Research Institution: Gunma University
• Principal Investigator: SATO Takashi Gunma University, 生体調節研究所, 助教 (70344934)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,790,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥690,000); Fiscal Year 2008: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000); Fiscal Year 2007: ¥800,000 (Direct Cost: ¥800,000)
• Keywords: Rab8 / 上皮細胞 / 遺伝子欠損マウス / 極性 / トランスポーター / KOマウス / 極性輸送 / Rab8a

Research Abstract

細胞極性の維持には、蛋白質を各々の細胞膜領域へと運ぶ極性輸送が必須である。今までに作製した様々な極性輸送制御分子のKOマウスのうち、Rab8aKOマウスは小腸上皮細胞のapicalへの輸送に異常が現れ、微絨毛萎縮症との関連も示唆された。本研究では、Rab8aに類似したRab8bの機能の解明のため、Rab8b欠損マウスと、Rab8aとRab8bの両方を欠損するマウスの解析を行った。また、apical輸送におけるRab8aの分子機序解明のため、Rab8aと他のapical輸送関連分子の間での相互作用の検討も行った。

Research Products

• [Journal Article] p31 deficiency influences endoplasmic reticulum tubular morphology and cell survival (2009)
  • Author(s): Uemura T, Sato T(共筆頭著者), Aoki T, Yamamoto A, Okada T, Hirai R, Harada R, Mori K, Tagaya M, and Harada A
  • Journal Title: Mol. Cell. Biol 29 Pages: 1869-1881
  • NAID: 40019431649
  • Peer Reviewed
• [Journal Article] Involvement of proton-sensing TDAG8 in extracellular acidification-induced inhibition of proinflammatory cytokine production in peritoneal macrophages (2009)
  • Author(s): Mogi C, Tobo M, Tomura H, Murata N, He XD, Sato K, Kimura T, Ishizuka T, Sasaki T, Sato T, Kihara Y, Ishii S, Harada A, and Okajima F
  • Journal Title: J. Immunol 182 Pages: 3243-3251
  • Peer Reviewed
• [Journal Article] SAP-1 is a microvillus-specific protein tyrosine phosphatase that modulates intestinal tumorigenesis (2009)
  • Author(s): Sadakata H, Okazawa H, Sato T, Supriatna Y, Ohnishi H, Kusakari S, Murata Y, Ito T, Nishiyama U, Minegishi T, Harada A, and Matozaki T
  • Journal Title: Genes Cells 14 Pages: 295-308
  • Peer Reviewed
• [Journal Article] Investigation of the role of oligopeptide transporter PEPT1 and sodium/glucose cotransporter SGLT1 in intestinal absorption of their substrates using small GTP-binding protein Rab8-null mice (2009)
  • Author(s): Kato Y, Sugiura T, Nakadera Y, Sugiura M, Kubo Y, Sato T, Harada A, and Tsuji A
  • Journal Title: Drug Metab. Dispos 37 Pages: 602-607
  • Peer Reviewed
• [Journal Article] Investigation of the role of oligopeptide transporter PEPT1 and sodium/glucose cotransporter SGLT1 in intestinal absorption of their substrates using small GTP-binding nrotein Rab8-null mice (2009)
  • Author(s): Yukio Kato
  • Journal Title: Drug metabolism and disposition 37 Pages: 602-607
  • Peer Reviewed
• [Journal Article] SAP-1 is a microvillus-specific protein tyrosine phosphatase that modulates intestinal tumorigenesis (2009)
  • Author(s): Hisanobu Sadakata
  • Journal Title: Genes to cells 14 Pages: 295-308
  • Peer Reviewed
• [Journal Article] Involvement of proton-sensing TDAG8 in extracellular acidification-induced inhibition of proinflamrnatory cytokine production in peritoneal macrophages (2009)
  • Author(s): Chihiro Mogi
  • Journal Title: The journal of immunology 182 Pages: 3243-3251
  • Peer Reviewed
• [Journal Article] p31 deficiency influences endoplasmic reticulum tubular morphology and cell survival (2009)
  • Author(s): Takefumi Uemura
  • Journal Title: Molecular and cellular biology 29 Pages: 1869-4881
  • Peer Reviewed
• [Journal Article] Neuron-specific and inducible recombination by Cre recombinase in the mouse. (2008)
  • Author(s): Hashimoto Y, Muramatsu K, Uemura T, Harada R, Sato T, Okamoto K, and Harada A
  • Journal Title: Neuroreport 19 Pages: 621-624
  • Peer Reviewed
• [Journal Article] Neuron-specific recombination by Cre recombinase inserted into the murine tau locus (2008)
  • Author(s): Muramatsu K, Hashimoto Y, Uemura T, Kunii M, Harada R, Sato T, Morikawa A, and Harada, A
  • Journal Title: Biochem. Biophys. Res. Commun 370 Pages: 419-423
  • Peer Reviewed
• [Journal Article] Neuron-specific recombination by Cre recombinase inserted into the murinetau locus (2008)
  • Author(s): Kazuhiro Muramatsu
  • Journal Title: Biochemical and biophysical research communications 370 Pages: 419-423
  • Peer Reviewed
• [Journal Article] Neuron-specific and inducible recombination by Cre recombinase in the mouse (2008)
  • Author(s): Yukiko Hashimoto
  • Journal Title: Neuroreport 19 Pages: 621-624
  • Peer Reviewed
• [Journal Article] Transcriptional induction of mammalian ER quality control proteins is mediated by single or combined action of ATF6alpha and XBP1 (2007)
  • Author(s): Yamamoto K, Sato T(共筆頭著), Matsui T, Sato M, Okada T, Yoshida H, Harada A, and Mori K
  • Journal Title: Dev. Cell 13 Pages: 365-376
  • Peer Reviewed
• [Journal Article] The Rab8 GTPase regulates apical protein localization in intestinal cells (2007)
  • Author(s): Sato T, Mushiake S, Kato Y, Sato K, Sato M, Takeda N, Ozono K, Miki K, Kubo Y, Tsuji A, Harada R, and Harada A
  • Journal Title: Nature 448 Pages: 366-369
  • Peer Reviewed
• [Journal Article] 14-3-3 proteins and protein phosphatases are not reduced in tau-deficient mice (2007)
  • Author(s): Fujio K, Sato M, Uemura T, Sato T, Sato-Harada R, amd Harada A
  • Journal Title: Neuroreport 18 Pages: 1049-1052
  • Peer Reviewed
• [Journal Article] The Rab8 GTPase regulates apical protein localization in intestinal cells. (2007)
  • Author(s): Takashi Sato
  • Journal Title: Nature 448 Pages: 366-369
  • Peer Reviewed
• [Journal Article] Transcriptional induction of mammalian ER quality control proteins is mediated by single or combined action of ATF6alpha and XBP1. (2007)
  • Author(s): Keisuke Yamamoto
  • Journal Title: Developmental Cell 13 Pages: 365-376
  • Peer Reviewed
• [Journal Article] 14-3-3proteins and protein phosphatases are not reduced in tau-deficient mice. (2007)
  • Author(s): Katsunori Fujio
  • Journal Title: Neuroreport 18 Pages: 1049-1052
  • Peer Reviewed
• [Presentation] 細胞極性の維持におけるRab8aおよびRab8bの役割 (2009)
  • Author(s): 佐藤隆史
  • Organizer: 第114回日本解剖学会総会
  • Place of Presentation: 岡山
  • Year and Date: 2009-03-28
• [Presentation] Rab8は小腸上皮におけるアピカル蛋白の局在と微絨毛の維持に必須である (2008)
  • Author(s): 佐藤隆史
  • Organizer: 第60回日本細胞生物学会大会
  • Place of Presentation: 横浜
  • Year and Date: 2008-06-30
• [Presentation] 極性輸送制御因子Rab8は、離乳後の小腸吸収上皮細胞apical蛋白の局在と栄養吸収機能に必須である (2008)
  • Author(s): 佐藤隆史
  • Organizer: 第113回日本解剖学会総会
  • Place of Presentation: 大分
  • Year and Date: 2008-03-28
• [Presentation] 極性輸送制御因子Rab8は、離乳後の小腸吸収上皮細胞apical蛋白の局在と栄養吸収機能に必須である (2008)
  • Author(s): 佐藤 隆史
  • Organizer: 日本解剖学会
  • Place of Presentation: 大分大学 医学部
  • Year and Date: 2008-03-28
• [Presentation] 極性輸送制御因子Rab8は、離乳後の小腸吸収上皮細胞apical蛋白の局在と栄養吸収機能に必須である (2007)
  • Author(s): 佐藤隆史
  • Organizer: 第30回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-12
• [Presentation] 極性輸送制御因子Rab8は、離乳後の小腸吸収上皮細胞apical蛋白の局在と栄養吸収機能に必須である (2007)
  • Author(s): 佐藤 隆史
  • Organizer: 日本分子生物学会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2007-12-12
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