Functional analysis of APC in neurite outgrowth

• Project/Area Number: 19770162
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Cell biology
• Research Institution: The University of Tokyo
• Principal Investigator: KAWASAKI Yoshihiro The University of Tokyo, 分子細胞生物学研究所, 助教 (10376642)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,760,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥360,000); Fiscal Year 2008: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2007: ¥2,200,000 (Direct Cost: ¥2,200,000)
• Keywords: 細胞骨格 / 運動 / APC / ユビキチン化 / NGF / Asef / Neurodap1 / 神経突起伸長

Research Abstract

APC結合蛋白質として見出したAsefは、各種dominant-negative変異体、及びshRNAを用いた解析によりPC12細胞におけるNGF刺激依存的な神経突起伸長に重要であることを見出した。さらに、蛍光イメージングを用いた解析によりAsefとAPCの細胞膜近傍への集積動態が異なることから、細胞膜近傍に集積したAPCがAsefと複合体を形成し、Asefの活性を制御していることが示唆された。

Research Products

• [Journal Article] Asef2 and Neurabin2 cooperatively regulat actin cytoskeletal organization and are involved in HGF-induced cell migration (2009)
  • Author(s): Sagara M, Kawasaki Y, Iemura SI, Natsume T, Takai Y, Akiyama T
  • Journal Title: Oncogene 28 Pages: 1357-1365
  • Peer Reviewed
• [Journal Article] Ligand-dependent interaction between estrogen receptor alpha and adenomatous polyposis coli (2008)
  • Author(s): Kouzmenko AP, Takeyama K, Kawasaki Y, Akiyama T, Kato S
  • Journal Title: Genes Cells 13 Pages: 723-730
  • Peer Reviewed
• [Journal Article] Truncation mutations abolish chromatin-associated activities of adenomatous polyposis coli (2008)
  • Author(s): Kouzmenko AP, Takeyama K, Kawasaki Y, Akiyama T, Kato S
  • Journal Title: Oncogene 27 Pages: 4888-4899
  • Peer Reviewed
• [Journal Article] Identification and Characterization of Asef2, a guanine-nucleotide exchange factor specific for Rac1 and Cdc42 (2007)
  • Author(s): Kawasaki Y., Sagara M., Shibata Y., Shirouzu M., Yokoyama S., Akiyama T
  • Journal Title: Oncogene 26 Pages: 7620-7627
  • Peer Reviewed
• [Journal Article] PH domain-mediated membrane targeting of Asef (2007)
  • Author(s): Muroya K., Kawasaki Y., Hayashi T., Ohwada S., Akiyama.T
  • Journal Title: Biochem. Biophys. Res. Commun 355 Pages: 85-88
  • Peer Reviewed
• [Journal Article] Crystal structure of the rac activator, asef, reveals its autoinhibitory mechanism (2007)
  • Author(s): Murayama K., Shirouzu M., Kawasaki Y., Kato-Murayama M., Hanawa-Suetsugu K., Sakamoto A., Katsura Y., Suenaga A., Toyama M., Terada T., Taiji M., Akiyama T., Yokoyama S
  • Journal Title: J. Biol. Chem 282 Pages: 4238-4242
  • Peer Reviewed
• [Journal Article] Identification and characterization of Asef2, a guanine-nucleotide exchange factor specific for Rac1 and Cdc42. (2007)
  • Author(s): Kawasaki. Y, et. al.
  • Journal Title: Oncogene 26 Pages: 7620-7627
  • Peer Reviewed
• [Presentation] APCの異常と発癌 (2009)
  • Author(s): 川崎善博
  • Organizer: 九州大学グローバルCOE「Wnt シグナルシンポジウム」
  • Place of Presentation: 福岡
• [Presentation] 癌抑制遺伝子APCの異常を介したポリープ形成におけるAsef/Asef2の関わり (2008)
  • Author(s): 川崎善博
  • Organizer: 日本分子生物学会
  • Place of Presentation: 神戸
• [Presentation] Role of the APC-associated guanine nucleotide exchange factor Asef/Asef2 in intestinal adenoma formation (2008)
  • Author(s): 川崎善博
  • Organizer: 日本癌学会
  • Place of Presentation: 名古屋
• [Presentation] Role of the APC-associated guanine nucleotide exchange factor Asef in signal transduction and angiogenesis (2008)
  • Author(s): 川崎善博
  • Organizer: 日本細胞生物学会
  • Place of Presentation: 横浜
• [Presentation] Role of the APC-associated guanine nucleotide exchange factor Asef in signal transduction and angiogenesis (2007)
  • Author(s): 川崎 善博
  • Organizer: 癌学会
  • Place of Presentation: 横浜
  • Year and Date: 2007-10-03
• [Presentation] Role of the APC-associated guanine nucleotide exchange factor Asef in signal transduction and angiogenesis (2007)
  • Author(s): 川崎善博
  • Organizer: 日本癌学会
  • Place of Presentation: 横浜