Proteins degradation pathways exist around lipid droplets

• Project/Area Number: 19770166
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Cell biology
• Research Institution: Nagoya University
• Principal Investigator: OHSAKI Yuki Nagoya University, 大学院・医学系研究科, 助教 (00378027)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,820,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥420,000); Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2007: ¥2,000,000 (Direct Cost: ¥2,000,000)
• Keywords: 脂質滴 / Apolipoprotein B / VLDL / プロテアソーム / 小胞体

Research Abstract

肝細胞では脂質滴(Lipid droplet : LD)由来の脂質とApolipoprotein B (ApoB)とが小胞体で結合して超低比重リポプロテイン(VLDL)が合成される. 本研究は (1)脂肪滴と小胞体の一部(ApoBが貯留する)が融合した構造(ApoB-crescent)がプロテアソーム阻害剤処理時に増加する事, (2)脂質滴画分には多くのプロテアソーム分解系関連因子が存在する事を肝癌由来培養細胞を用いて明らかにし, 脂質滴周辺でのApoB代謝機構の存在を示唆した.

Research Products

• [Journal Article] Lipid droplet-associated proteins protect renal tubular cells from fatty acid-induced apoptosis (2008)
  • Author(s): Urahama Y, Ohsaki Y, Fujita Y, Maruyama S, Yuzawa Y, Matsuo S, Fujimoto T
  • Journal Title: The American Journal of Pathology 173(5) Pages: 1286-1294
  • Peer Reviewed
• [Journal Article] Lipid droplets: a classic organelle with new outfits (2008)
  • Author(s): Fujimoto T, Ohsaki Y, Cheng J, Suzuki M, Shinohara Y
  • Journal Title: Histochem Cell Biol 130(2) Pages: 263-279
  • Peer Reviewed
• [Journal Article] Platelet-derived growth factor signals play critical roles in differentiation of longitudinal smooth muscle cells in mouse embryonic gut. (2008)
  • Author(s): Kurahashi M, Niwa Y, Cheng J, Ohsaki Y, Fujita A., Goto H, Fujimoto Y, Torihashi S.
  • Journal Title: Neurogastroenterol Motil 20(5) Pages: 521-531
  • Peer Reviewed
• [Journal Article] Lipid droplets are arrested in the ER membrane by tight binding of lipidated apolipoprotein B-100. (2008)
  • Author(s): Ohsaki Y, Cheng J, Suzuki M, Fujita A, Fujimoto T.
  • Journal Title: J Cell Sci 121(14) Pages: 2415-2422
  • Peer Reviewed
• [Journal Article] 脂肪滴形成のメカニズム (2008)
  • Author(s): 藤本 豊士, 大崎 雄樹, 鈴木 倫毅
  • Journal Title: 膜 MEMBRANE 33(1) Pages: 17-23
  • NAID: 10020130439
• [Journal Article] Biogenesis of cytoplasmic lipid droplets : From the lipid ester globule in the membrane to the visible structure (2008)
  • Author(s): 大崎雄樹
  • Journal Title: Biochimica et Biophysica Acta (In press)
  • Peer Reviewed
• [Journal Article] Lipid droplet-associated proteins protect renal tubular cells from fatty acid-induced apoptosis (2008)
  • Author(s): 浦濱善倫
  • Journal Title: The American Journal of Pathology 173 Pages: 1286-1294
  • Peer Reviewed
• [Journal Article] Lipid droplets : a classic organelle with new outfits (2008)
  • Author(s): 藤本豊士
  • Journal Title: Histochemistry and Cell Biology 130 Pages: 263-279
  • Peer Reviewed
• [Journal Article] Lipid droplets are arrested in the ER membrane by tight binding of lipidated apolipoprotein B-100 (2008)
  • Author(s): 大崎雄樹
  • Journal Title: Journal of Cell Science 121 Pages: 2415-2422
  • Peer Reviewed
• [Journal Article] Platelet-derived growth factor signals play critical roles in differentiation of longitudinal smooth muscle cells in mouse embryonic gut. (2008)
  • Author(s): 倉橋 正明
  • Journal Title: Neurogastroenterology and motilily (In press)
  • Peer Reviewed
• [Journal Article] 脂肪滴形成のメカニズム (2008)
  • Author(s): 藤本 豊士
  • Journal Title: 膜 MEMBRANE 33 Pages: 17-23
• [Journal Article] All-trans-retinol generated by rhodopsin photobleaching induces rapid recruitment of TIP47 to lipid droplets in the retinal pigment epithelium. (2007)
  • Author(s): Tsuiki E, Fujita A, Ohsaki Y, Cheng J, Irie T, Yoshikawa K, Senoo H, Mishima K, Kitaoka T, Fujimoto T
  • Journal Title: Invest Ophthalmol Vis Sci 48(6) Pages: 2858-2867
  • Peer Reviewed
• [Journal Article] All-trans-retinol generated by rhodopsin photobleaching induces rapid recruitment of TIP47 t0 lipid droplets in the retinal pigment epithelium. (2007)
  • Author(s): 築城 英子
  • Journal Title: Investigative ophthalmology & visual science 48 Pages: 2858-2867
  • Peer Reviewed
• [Journal Article] Biogenesis of cytoplasmic lipid droplets: From the lipid ester globule in the membrane to the visible structure
  • Author(s): Ohsaki Y, Cheng J, Suzuki M, Shinohara Y, Fujita A, Fujimoto T
  • Journal Title: Biochim Biophys Acta 2008 印刷中
  • Peer Reviewed
• [Presentation] 脂肪滴に局在するユビキチン化関連蛋白質の解析 (2009)
  • Author(s): 大崎雄樹, 程晶磊, 鈴木倫毅, 藤本豊士
  • Organizer: 第114回日本解剖学会総会・全国学術集会
  • Place of Presentation: 岡山理科大学(岡山市)
  • Year and Date: 2009-03-28
• [Presentation] ADP-ribosylationによるApoB代謝制御 (2008)
  • Author(s): 大崎雄樹, 程晶磊, 鈴木倫毅, 藤本豊士
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会合同大会
  • Place of Presentation: 神戸ポートアイランド(神戸市)
  • Year and Date: 2008-12-12
• [Presentation] ADPリボシル化阻害によるApoB分泌抑制 (2008)
  • Author(s): 大崎雄樹, 程晶磊, 鈴木倫毅, 藤本豊士.
  • Organizer: 第49回日本組織細胞化学会総会・学術集会
  • Place of Presentation: 長崎大学医学部(長崎市)
  • Year and Date: 2008-10-06
• [Presentation] Brefeldin A処理による小胞体内腔での脂肪滴形成 (2008)
  • Author(s): 大崎雄樹, 程晶磊, 鈴木倫毅, 藤本豊士
  • Organizer: 第113回日本解剖学会総会・全国学術集会
  • Place of Presentation: 大分大学医学部(由布市)
  • Year and Date: 2008-03-27
• [Presentation] Lipoprotein産生細胞における脂肪滴-小胞体密着構造の形成と意義 (2007)
  • Author(s): 大崎雄樹, 程晶磊, 鈴木倫毅, 藤田秋一, 藤本豊士
  • Organizer: 第30回日本分子生物学会年会・第80回日本生化学会大会合同大会
  • Place of Presentation: パシフィコ横浜(横浜市)
  • Year and Date: 2007-12-12
• [Presentation] Lipoprotein産生細胞における脂肪滴-小胞体密着構造の形成と意義 (2007)
  • Author(s): 大崎 雄樹
  • Organizer: 第30回日本分子生物学会年会・第80回日本生化学会大会 合同大会
  • Place of Presentation: パシフィコ横浜(横浜市)
  • Year and Date: 2007-12-12
• [Presentation] Budding of lipid droplets form ER is arrested by accumulation of lipidated Apolipoprotein B-100 (2007)
  • Author(s): Yuki Ohsaki, Jinglei Cheng, Michitaka Suzuki, Akikazu Fujita, and Toyoshi Fujimoto
  • Organizer: 科学研究費補助金特定領域研究「メンブレントラフィック」国際シンポジウム
  • Place of Presentation: 淡路夢舞台国際会議場(淡路市)
  • Year and Date: 2007-11-27
• [Presentation] Budding of lipid droplets form ER is arrested by accumulation of lipidated Apolipoprotein B-100 (2007)
  • Author(s): 大崎 雄樹
  • Organizer: 科学研究費補助金特定領域研究「メンブレントラフィック」国際シンポジウム
  • Place of Presentation: 淡路夢舞台国際会議場(兵庫県淡路市)
  • Year and Date: 2007-11-27
• [Presentation] 肝癌由来培養細胞における脂質清-小胞体密着構造の解析 (2007)
  • Author(s): 大崎 雄樹
  • Organizer: 日本解剖学会第67回中部支部学術集会
  • Place of Presentation: 愛知医科大学(愛知県長久手町)
  • Year and Date: 2007-11-13
• [Presentation] 肝癌由来培養細胞における脂質滴-小胞体密着構造の解析 (2007)
  • Author(s): 大崎雄樹, 程晶磊, 鈴木倫毅, 藤田秋一, 藤本豊士
  • Organizer: 日本解剖学会第67回中部支部学術集会
  • Place of Presentation: 愛知医科大学(愛知県長久手町)
  • Year and Date: 2007-10-13
• [Presentation] Lipid droplets interact with ER in lipoprotein secretory cells (2007)
  • Author(s): 大崎雄樹, 程晶磊, 鈴木倫毅, 藤田秋一, 藤本豊士
  • Organizer: 第48回日本組織細胞化学会総会・学術集会
  • Place of Presentation: 甲府市総合市民会館(甲府市)
  • Year and Date: 2007-09-29
• [Presentation] Lipid droplets interact with ER in lipoprotein secretory cells (2007)
  • Author(s): 大崎 雄樹
  • Organizer: 第48回日本組織細胞化学会総会・学術集会
  • Place of Presentation: 甲府市総合市民会館(山梨県甲府市)
  • Year and Date: 2007-09-29
• [Presentation] Formation of lipid droplet-ER fusion structures in hepatocytes (2007)
  • Author(s): 大崎 雄樹
  • Organizer: 第40回日本発生生物学会・第59回日本細胞生物学会 合同大会
  • Place of Presentation: 福岡国際会議場(福岡市)
  • Year and Date: 2007-05-30