Pluripotency of Human ES cells : Study of cell polarity and symmetric division regulating pluripotency

• Project/Area Number: 19770172
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Cell biology
• Research Institution: Kyoto University
• Principal Investigator: SUMI Tomoyuki Kyoto University, 再生医科学研究所, 助教 (90378894)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,670,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥270,000); Fiscal Year 2008: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2007: ¥2,500,000 (Direct Cost: ¥2,500,000)
• Keywords: 細胞内・細胞間情報伝達 / ヒトES細胞 / 多能性 / Wnt / beta-catenin / 中胚葉 / ヒトSE細胞

Research Abstract

本研究は、ヒトES細胞が密着結合有する上皮細胞様の頂端部-基底部に沿った非対称性を有していることに着目し、細胞極性と多能性維持・初期分化誘導の関連性に焦点を当て研究を開始した。ヒトES細胞において細胞極性に制御するβ-cateninを特異的に活性化した結果、速やかに頂端部-基底部極性が消失し、哺乳類初期発生を模倣する一連の分化遺伝子の発現が誘導された。これらの結果は、ヒトES細胞の細胞極性の維持が多能性を維持する上で必須であることを示し、細胞極性の崩壊によって初期発生過程を模倣する遺伝子発現プログラムが惹起されることが明らかになった。

Research Products

• [Journal Article] Defining lineage specification of human embryonic stem cells by the orchestrated balance of canonical Wnt/b-catenin and BMP signaling (2008)
  • Author(s): Tomoyuki Sumi, Norihiro Tsuneyoshi, Norio Nakatsuji, Hirofumi Suemori
  • Journal Title: Development 135 Pages: 2969-2979
  • Peer Reviewed
• [Journal Article] PRDM14 suppresses expression of differentiation marker genes in human embryonic stem cells (2008)
  • Author(s): Norihiro Tsuneyoshi, Tomoyuki Sumi, Hiroaki Onda, Hiroshi Nojima, Norio Nakatsuji, Hirofumi Suemori
  • Journal Title: Biochem. Biophys. Res. Commun. 367 Pages: 899-905
  • Peer Reviewed
• [Journal Article] Defining early lineage specification of human embryonic stem cells by the orchestrated balance of canonical Wnt/β-catenin, Activin/Nodal, and BMP signaling (2008)
  • Author(s): Tomoyuki Sumi
  • Journal Title: Development 135 Pages: 2969-2979
  • Peer Reviewed
• [Journal Article] PRDM14 suppresses expression of differentiation marker genes in human embryonic stem cells. (2008)
  • Author(s): Norihiro Tsuneyoshi
  • Journal Title: Biochem. Biophys. Res. Commun. 367 Pages: 899-905
  • Peer Reviewed
• [Journal Article] Apoptosis and Differentiation of Human Embryonic Stem Cells Induced by Sustained Activation of c-Myc (2007)
  • Author(s): Tomoyuki Sumi, Norihiro Tsuneyoshi, Norio Nakatsuji, Hirofumi Suemori
  • Journal Title: Oncogene 26 Pages: 5564-5576
  • Peer Reviewed
• [Journal Article] Apoptosis and Differentiation of Human Embryonic Stem Cells Induced by Sustained Activation of c-Myc. (2007)
  • Author(s): Tomoyuki Sumi
  • Journal Title: Oncogene 26 Pages: 5564-5576
  • Peer Reviewed
• [Presentation] CANONICAL WNT/BETA-CATENIN-MEDIATED EARLY LINEAGE SPECIFICATION IN HUMAN EMBRYONIC STEM CELLS (2008)
  • Author(s): Tomoyuki Sumi
  • Organizer: 6th ISSCR (International Society for Stem Cell Research) Annual Meeting
  • Place of Presentation: Philadelphia, USA
• [Presentation] ヒトES細胞の多能性維持における古典的Wnt/beta-cateninシグナル伝達経路の役割 (2007)
  • Author(s): 角智行
  • Organizer: 第30回日本分子生物学会年会・第80回日本生化学会大会・合同大会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2007-12-12
• [Presentation] SUSTAINED ACTIVATION OF C-MYC DISTURBS SELF-RENEWAL OF HUMAN EMBRYONIC STEM CELLS (2007)
  • Author(s): Tomoyuki Sumi
  • Organizer: 5th ISSCR (International Society for Stem Cell Research) Annual Meeting
  • Place of Presentation: Cairns, Australia
• [Book] 「ヒトES細胞の現状と展望」, 分子細胞治療第6巻第3号 (2007)
  • Author(s): 角智行