• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Study on the regulation of endothelial cell-cell adhesions by Rap1 small GTPase and its biological functions

Research Project

Project/Area Number 19770185
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Cell biology
Research InstitutionNational Cardiovascular Center Research Institute

Principal Investigator

FUKUHARA Shigetomo  National Cardiovascular Center Research Institute, 循環器形態部, 室長 (70332880)

Project Period (FY) 2007 – 2008
Project Status Completed (Fiscal Year 2008)
Budget Amount *help
¥3,840,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥540,000)
Fiscal Year 2008: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2007: ¥1,500,000 (Direct Cost: ¥1,500,000)
Keywords細胞内 / 細胞間情報伝達 / VE-cadherin / 細胞間接着 / Rap1 / アクチン細胞骨格 / FRAP解析 / 細胞内小胞輸送
Research Abstract

全身の細胞に酸素や栄養を供給する血管の内腔は、血管内皮細胞によって覆われている。内皮細胞は互いに接着し血液が外に漏れないようにバリヤーとして機能しているが、炎症時や虚血によって新たに血管が形成されるときにはそのバリヤー機能は低下する。本研究では血管のバリヤー機能を制御するメカニズムを解明するため、内皮細胞間の接着を制御するVascular Endothelial(VE)-Cadherinに注目し、その接着活性を調節するシグナル伝達系を解析した。その結果、Rap1低分子量G蛋白質がアクチン細胞骨格系を制御することによりVE-cadherin接着を増強することが明らかになった。

Report

(3 results)
  • 2008 Annual Research Report   Final Research Report ( PDF )
  • 2007 Annual Research Report
  • Research Products

    (38 results)

All 2009 2008 2007 Other

All Journal Article (18 results) (of which Peer Reviewed: 18 results) Presentation (13 results) Book (6 results) Remarks (1 results)

  • [Journal Article] Tie2 is tied at the cell-cell contacts and to extracellular matrix by Angiopoietin-1.2009

    • Author(s)
      S. Fukuhara, K. Sako, K. Noda, K. Nagao, K. Miura, N. Mochizuki
    • Journal Title

      Exp. Mol. Med 41

      Pages: 133-139

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Angiopoietin-1 induces Kruppel-Like Factor 2 expression through a phosphoinositide 3-kinase/AKT-dependent activation of myocyte enhancer factor 2.2009

    • Author(s)
      K. Sako, S. Fukuhara, M. Takashi, T. Hamakubo, H. Song, T. Kodama, A. Fukamizu, J. S. Gutkind, G. Y. Koh, N. Mochizuki
    • Journal Title

      J. Biol. Chem 284

      Pages: 5592-5601

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Angiopoietin-1 induces Kruppel-Like Factor 2 expression through a phosphoinositide 3-kinase/AKT-dependent activation of myocyte enhancer factor 2.2009

    • Author(s)
      K. Sako
    • Journal Title

      J. Biol. Chem. 284

      Pages: 5592-5601

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Differential function of Tie2 at cell-cell contacts and cell-substratum contacts regulated by angiopoietin-1.2008

    • Author(s)
      S. Fukuhara, K. Sako, T. Minami, K. Noda, H. Z. Kim, T. Kodama, M. Shibuya, N. Takakura, G. Y. Koh, N. Mochizuki
    • Journal Title

      Nat. Cell Biol 10

      Pages: 513-526

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Cryptide signaling: amphiphilic peptide-induced exocytosis mechanisms in mast cells.2008

    • Author(s)
      H. Mukai, M. Kikuchi, S. Fukuhara, Y. Kiso, E. Munekata
    • Journal Title

      Biochem. Biophys. Res. Commun 375

      Pages: 22-26

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Single common mutation in the cardiac sodium channel gene SCN5A with diverse clinical phenotypes.2008

    • Author(s)
      N. Makita, E. Behr, W. Shimizu, M. Horie, A. Sunami, L. Crotti, E. Schulze-Bahr, S. Fukuhara, N. Mochizuki, T. Makiyama, H. Itoh, M. Christiansen, P. McKeown, K. Miyamoto, S. Kamakura, H. Tsutsui, P. J. Schwartz, A. L. George Jr, D. M. Roden.
    • Journal Title

      J. Clin. Invest 118

      Pages: 2219-2229

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Conformational switch of angiotensin II type 1 receptor underlying mechanical stress-induced activation.2008

    • Author(s)
      N. Yasuda, S. Miura, H. Akazawa, T. Tanaka, Y Qin, Y. Kiya, S. Imaizumi, M. Fujino, K. Ito, Y. Zou, J. Ge, S. Fukuhara, S. Kunimoto, N. Mochizuki, K. Fukuzaki, T. Sato, H. Nakaya, K. Saku, I. Komuro
    • Journal Title

      EMBO Rep 9

      Pages: 179-186

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Differential function of Tie2 at cell-cell contacts and cell-substratum contacts regulated by angiopoietin-1.2008

    • Author(s)
      S. Fukuhara
    • Journal Title

      Nat. Cell Biol. 10

      Pages: 513-526

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Cryptide signaling : amphiphilic peptide-induced exocytosis mechanisms in mast cells.2008

    • Author(s)
      H. Mukai
    • Journal Title

      Biochem. Biophys. Res. Commun. 375

      Pages: 22-26

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Single common mutation in the cardiac sodium channel gene SCN5A with diverse clinical phenotypes.2008

    • Author(s)
      N. Makita
    • Journal Title

      J. Clin. Invest. 118

      Pages: 2219-2229

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Conformational switch of angiotensin II type 1 receptor underlying mechanical stressinduced activation.2008

    • Author(s)
      N. Yasuda
    • Journal Title

      EMBO Rep 9

      Pages: 179-186

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Protein Tyrosine Kinase 2β as a Candidate Gene for Hypertension.2007

    • Author(s)
      K. Kamide, Y. Kokubo, S. Fukuhara, H. Hanada, J. Yang, A. Kada, J. Nagura, S. Takiuchi, T. Horio, Y. Kawano, A. Okayama, H. Tomoike, T. Miyata
    • Journal Title

      Pharmacogenet. Genomics 17

      Pages: 931-939

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] ・-defensin overexpression induces progressive muscle degeneration in mice.2007

    • Author(s)
      Y. Yamaguchi, T. Nagase, T. Tomita, K. Nakamura, S. Fukuhara, T. Amano, H. Yamamoto, Y. Ide, M. Suzuki, S. Teramoto, T. Asano, K. Kangawa, N. Nakagata, Y. Ouchi, H. Kurihara
    • Journal Title

      Am. J. Physiol. Cell Physiol 292

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Protein Tyrosine Kinase 2β as a Candidate Gene for Hypertension2007

    • Author(s)
      K. Kamide
    • Journal Title

      Pharmacogenet.Genomics. 17

      Pages: 931-939

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] □-defensin over expression induces progressive mucle degeneration in mice2007

    • Author(s)
      Y. Yamaguchi
    • Journal Title

      J.Physiol.Cell Physiol. 292

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Tie2 is tied at the cell-cell contacts and to extracellular matrix by Angiopoietin-1.

    • Author(s)
      S. Fukuhara
    • Journal Title

      Exp. Mol. Biol. (In press)

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Differential function of Tie2 at cell-cell colltacts and cen-substratum contacts regulated by angiopoietin-1

    • Author(s)
      S. Fukuhara
    • Journal Title

      Nat.Cell Biol. in press

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Single common mutation in the cardiac sodium channel gene SCN5A with diverse clinical phenotypes

    • Author(s)
      N. Makita
    • Journal Title

      J. Clip, Invest. in press

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Presentation] Angiostasis and angiogenesis regulated by angiopoietin-1/Tie2 receptor system2009

    • Author(s)
      Shigetomo Fukuhara
    • Organizer
      Japan-Mexico Workshop
    • Place of Presentation
      Mexico city, Mexico
    • Year and Date
      2009-02-25
    • Related Report
      2008 Final Research Report
  • [Presentation] アンジオポエチン-1/Tie2 受容体シグナルの空間的・機能的制御2009

    • Author(s)
      福原茂朋
    • Organizer
      第5回宮崎サイエンスキャンプ
    • Place of Presentation
      フェニックスシーガイアリゾート宮崎
    • Year and Date
      2009-02-20
    • Related Report
      2008 Final Research Report
  • [Presentation] アンジオポエチン-1/Tie2受容体シグナルの空間的・機能的制御2009

    • Author(s)
      福原 茂朋
    • Organizer
      第5回宮崎サイエンスキャンプ
    • Place of Presentation
      フェニックスシーガイアリゾート宮崎
    • Year and Date
      2009-02-20
    • Related Report
      2008 Annual Research Report
  • [Presentation] ANGIOSTASIS AND ANGIOGENESIS REGULATED BY ANGIOPOIETIN-1/TIE2 RECEPTOR SYSTEM2009

    • Author(s)
      S. Fukuhara
    • Organizer
      Japan-Mexico Workshop
    • Place of Presentation
      Mexico city, Mexico
    • Year and Date
      2009-02-02
    • Related Report
      2008 Annual Research Report
  • [Presentation] Angiostasis and angiogenesis regulated by angiopoietin1-Tie2 signaling2008

    • Author(s)
      Shigetomo Fukuhara
    • Organizer
      The 6th Korea-Japan Joint Symposium on Vascular Biology & The 16th Annual Meeting of the Japanese Vascular Biology and Medicine Organization
    • Place of Presentation
      金沢市石川県立音楽堂
    • Year and Date
      2008-12-05
    • Related Report
      2008 Annual Research Report 2008 Final Research Report
  • [Presentation] アンジオポエチン-1/Tie2 受容体シグナルによる血管安定化・血管新生制御機構2008

    • Author(s)
      福原茂朋
    • Organizer
      生理研研究会「イオンチャネル・トランスポーターと心血管機能 : 学際的取り組みによる新戦略」
    • Place of Presentation
      岡崎市自然科学研究機構 岡崎コンファレンスセンター
    • Year and Date
      2008-11-20
    • Related Report
      2008 Final Research Report
  • [Presentation] アンジオポエチン-1/Tie2受容体シグナルによる血管安定化・血管新生制御機構2008

    • Author(s)
      福原 茂朋
    • Organizer
      生理研研究会「イオンチャネル・トランスポーターと心血管機能 : 学際的取り組みによる新戦略」
    • Place of Presentation
      岡崎市自然科学研究機構岡崎コンファレンスセンター
    • Year and Date
      2008-11-20
    • Related Report
      2008 Annual Research Report
  • [Presentation] Angiostatic and Angiogenic Regulation by Intercellular Trans-associated Tie2 and ECM-anchored Tie22008

    • Author(s)
      Shigetomo Fukuhara
    • Organizer
      67th Annual Meeting of the Japanese Cancer Association
    • Place of Presentation
      名古屋国際会議場
    • Year and Date
      2008-10-28
    • Related Report
      2008 Annual Research Report 2008 Final Research Report
  • [Presentation] Angiopoietin-1 Regulates Distinct Signaling Functions through Trans-associated Tie2 at Cell-cell Contacts and Extracellular Matrix-anchored Tie2 at Cell-substratum Contacts2008

    • Author(s)
      Shigetomo Fukuhara
    • Organizer
      15th International Vascular Biology Meeting
    • Place of Presentation
      Sydney, Australia
    • Year and Date
      2008-06-05
    • Related Report
      2008 Annual Research Report 2008 Final Research Report
  • [Presentation] アンジオポエチン-1によって活性化されたTie2 受容体は細胞間および細胞-基質間接着部位に移動し異なったシグナルを活性化する2007

    • Author(s)
      福原茂朋
    • Organizer
      第30回日本分子生物学会、第80回日本生化学会合同年会
    • Place of Presentation
      横浜パシフィコ横浜
    • Year and Date
      2007-12-12
    • Related Report
      2008 Final Research Report
  • [Presentation] アンジオポエチンー1によって活性化されたTie2受容体は細胞間および細胞-基質間接着部位に移動し異なったシグナルを活性化する2007

    • Author(s)
      福原 茂朋
    • Organizer
      第30回日本分子生物学会、第80回日本生化学会合同年会
    • Place of Presentation
      横浜パシフィコ横浜
    • Year and Date
      2007-12-12
    • Related Report
      2007 Annual Research Report
  • [Presentation] アンジオポエチン-1/Tie2 受容体シグナルの空間的制御とその血管形成における役割2007

    • Author(s)
      福原茂朋
    • Organizer
      第11回Molecular Cardiovascular Conference
    • Place of Presentation
      北海道キロロリゾート
    • Year and Date
      2007-09-16
    • Related Report
      2008 Final Research Report
  • [Presentation] アンジオポエチンー1によって活性化されたTie2受容体は細胞間および細胞-基質問接着部位に移動し異なったシグナルを活性化する2007

    • Author(s)
      福原 茂朋
    • Organizer
      第11回 Molecular Cardiovascular Conference
    • Place of Presentation
      北海道キロロリゾート
    • Year and Date
      2007-09-16
    • Related Report
      2007 Annual Research Report
  • [Book] 実験医学(Current Topics)2008

    • Author(s)
      福原茂朋、望月直樹
    • Publisher
      羊土社
    • Related Report
      2008 Final Research Report
  • [Book] 実験医学 増刊(生細胞を使ったライブイメージングでみえる生命現象)2008

    • Author(s)
      福原茂朋、望月直樹
    • Publisher
      羊土社
    • Related Report
      2008 Final Research Report
  • [Book] 循環器病研究の進歩2008

    • Author(s)
      福原茂朋、望月直樹
    • Publisher
      協和企画
    • Related Report
      2008 Final Research Report
  • [Book] 実験医学(Current Topics)2008

    • Author(s)
      福原 茂朋
    • Publisher
      羊土社
    • Related Report
      2008 Annual Research Report
  • [Book] 実験医学増刊(生細胞を使ったライブイメージングでみえる生命現象)2008

    • Author(s)
      福原 茂朋
    • Publisher
      羊土社
    • Related Report
      2008 Annual Research Report
  • [Book] 循環器病研究の進歩(Angiopoietin-Tie2によるangiostasisとangiogenesis制御)2008

    • Author(s)
      福原 茂朋
    • Publisher
      協和企画
    • Related Report
      2008 Annual Research Report
  • [Remarks] 本研究課題によって得られた研究成果が、2008年4月21日、日経新聞および2008年4月21日、朝日新聞地方版夕刊で紹介された。

    • Related Report
      2008 Final Research Report

URL: 

Published: 2007-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi