Influence of co-culture with germ cellsthat exert on genomic imprinting of imprinted genes

• Project/Area Number: 19770188
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Developmental biology
• Research Institution: Gunma University
• Principal Investigator: HORII Takuro Gunma University, 生体調節研究所, 助教 (00361387)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,750,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥450,000); Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2007: ¥1,800,000 (Direct Cost: ¥1,800,000)
• Keywords: インプリンティング / 刷り込み / 始原生殖細胞 / ES細胞 / EG細胞 / 生殖原基 / インプリンテング / 始原生細胞

Research Abstract

生殖細胞の性特異的なゲノムの刷り込みはマウスの場合、妊娠8.5-12.5日の間に生じる。しかし、そのような修飾が遺伝的に決まっているのか、周囲の環境によって決まるのか明らかではない。我々は妊娠8.5日目の始原生殖細胞より樹立した生殖幹細胞を異性の生殖原基と培養することで性特異的なゲノムの刷り込みを変化させることができるのか調べた。その結果、少なくとも8.5日目の始原生殖細胞由来生殖幹細胞ではすでに運命が決定されていることが分かった。

Research Products

• [Journal Article] Derivation of Embryonic Stem Cells from Mouse Tetraploid Embryos and Their Character (2008)
  • Author(s): Horii T, Kimura M, Morita S, Nagao Y, and Hatada I
  • Journal Title: Biol Reprod. special issue Pages: 118-118
  • Peer Reviewed
• [Journal Article] Loss of Genomic Imprinting in Mouse Parthenogenetic Embryonic Stem Cells (2008)
  • Author(s): Horii T, Kimura M, Morita S, Nagao Y, Hatada I
  • Journal Title: Stem Cells 26 Pages: 79-88
  • Peer Reviewed
• [Journal Article] Derivation of Embryonic Stem Cells from Mouse Tetraploid Embryos and Their Character (2008)
  • Author(s): Horii T, Kimura M, Morita S, Yanagisawa E, Nagao Y, Hatada I
  • Journal Title: Biology of Reproduction (Special issue) Pages: 118-118
  • Peer Reviewed
• [Journal Article] Loss of Genomic Imprinting in Mouse Parthenogenetic Embryonic Stem Cells. (2008)
  • Author(s): Horii T, Kimura M, Morita S, Nagao Y, Hatada I
  • Journal Title: Stem Cells 26 Pages: 79-88
  • Peer Reviewed
• [Journal Article] Loss of imprints of parthenogenetic ES cells in murine chimeras (2007)
  • Author(s): Horii T, Kimura M Nagao Y and Hatada I
  • Journal Title: Reprod. Fetil. Dev 19 Pages: 228-228
  • Peer Reviewed
• [Presentation] マウス四倍体ES細胞の樹立とその性質 (2008)
  • Author(s): 堀居拓郎、木村美香、森田純代、柳澤永吉、長尾恭光、畑田出穂
  • Organizer: 第31回日本分子生物学会年会
  • Place of Presentation: 神戸
• [Presentation] Derivation of Embryonic Stem Cells from Mouse Tetraploid Embryos and Their Character (2008)
  • Author(s): Horii T, Kimura M, Morita S, Nagao Y, and Hatada I
  • Organizer: The 41st Society for the study of reproduction
  • Place of Presentation: Hawaii
• [Presentation] Derivation of Embryonic Stem Cells from Mouse Tetraploid Embr yos and Their Character (2008)
  • Author(s): Horii T, Kimura M, Morita S, Yanagisawa E, Nagao Y, Hatada I
  • Organizer: 41th annual meeting society for the study of reproduction
  • Place of Presentation: コナ、アメリカ合衆国