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Study of mechanism of metamorphosis by transcriptome analysis of the mutants of Ciona intestinalis

Research Project

Project/Area Number 19770193
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Developmental biology
Research InstitutionOkinawa Institute of Science and Technology (2008)
Kyoto University (2007)

Principal Investigator

HAMADA Mayuko  Okinawa Institute of Science and Technology, マリンゲノミックスユニット, 研究員 (40378584)

Project Period (FY) 2007 – 2008
Project Status Completed (Fiscal Year 2008)
Budget Amount *help
¥3,620,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥420,000)
Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2007: ¥1,800,000 (Direct Cost: ¥1,800,000)
Keywordsホヤ / マイクロアレイ / 突然変異体 / 変態 / 発生 / 発生・分化
Research Abstract

本研究ではカタユウレイボヤの変態過程の一部に異常が見られる変異体sj1, sj2, sj4 (trf : tail regression failure)において、どのような遺伝子発現の変化が見られるかをマイクロアレイを用いて解析した。その結果、体軸回転が進行するsj1では間充織で発現する遺伝子の増加が見られた。また、尾部吸収が起こらず、成体組織が成長するtrfでは神経系で発現する遺伝子の減少が示唆された。また、これらの変異体間において発現差が見られた遺伝子は、変異体ごとに異なっており、変態過程が独立の過程から起こることが遺伝子レベルでも示唆される結果となった。

Report

(3 results)
  • 2008 Annual Research Report   Final Research Report ( PDF )
  • 2007 Annual Research Report
  • Research Products

    (2 results)

All 2007

All Presentation (2 results)

  • [Presentation] Identification of Ciona intestinalis genes involved in metamorphosis by microarray analysis of swimming juvenile mutant2007

    • Author(s)
      Mayuko Hamada, Yasunori Sasakura, Nori Satoh
    • Organizer
      4th International Tunicate Meeting
    • Place of Presentation
      フランス・Villefranche-sur-Mer Marine Station
    • Related Report
      2008 Final Research Report
  • [Presentation] Identification of Ciona intestinalis genes involved in metamorphosis by microarray analysis of swimming juvenile mutant.2007

    • Author(s)
      Mayuko Hamada
    • Organizer
      4th international Tunicate Meeting
    • Place of Presentation
      Villefranche-sur-Mer, Observatoire Oceanologique Saint-Jean Cap Ferrat, France
    • Related Report
      2007 Annual Research Report

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Published: 2007-04-01   Modified: 2016-10-28  

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