Studies of the role on dominant negative form Importin13 upon mouse meiotic germ cell development

• Project/Area Number: 19770198
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Developmental biology
• Research Institution: Kumamoto University
• Principal Investigator: YAMAGUCHI Yasuka Kumamoto University, 発生医学研究センター, 研究員 (90448522)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,760,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥360,000); Fiscal Year 2008: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2007: ¥2,200,000 (Direct Cost: ¥2,200,000)
• Keywords: 生殖細胞 / 減数分裂 / Ipo13 / マウス / 組織培養 / 分子生物学 / 発生・分化

Research Abstract

細胞分化等を誘導する分子の細胞内局在変化の引き金となるImportin ファミリーの一つ、Ipo13遺伝子の減数分裂期生殖細胞細胞分化における役割について研究を進めた。全長(long)のIpo13(L-Ipo13)は標的タンパク質カーゴ分子であり核内で活性を示すubiquitin-conjugating enzyme 9(UBC9)の核局在を誘導する。しかし、精巣特異的発現を示す、核移行領域欠損型のTestis specific(TS)-Ipo13はUBC9の核への局在化を誘導しなかった。さらに両者の共発現ではL-Ipo13のUBC9の輸送活性が阻害されることを観察した。また、UBC9が核局在を示さない条件下では、生殖細胞の減数分裂の進行が遅れる様子を観察した。以上の観察から、転写開始点の使い分けによって得られると推測されるTS-IPO13は、Ipo13遺伝子の全長産物であるL-IPO13の細胞質核間輸送活性を低下させ、UBC9などの標的カーゴ分子の核蓄積を阻害して、減数分裂期の生殖細胞の分化を制御していると結論づけた。

Research Products

• [Journal Article] Analyzing gene function in whole mouse embryo and fetal organ in vitro
  • Author(s): Satomi S. Tanaka
  • Journal Title: Methods in Molecular Biology (Mark Lewandoski ed.) (In press)
• [Presentation] Stage-specific Importin13 activity regulates meiosis of germ cells in the mouse (2008)
  • Author(s): 山口泰華
  • Organizer: 日本発生生物学会 (JSDB)
  • Place of Presentation: The Tokushima Arts Foundation for Culture (徳島県郷土文化会館)
  • Year and Date: 2008-05-29
• [Presentation] Sall4 is essential for development of mouse primordial germ cells (2008)
  • Author(s): Yasuka L. Yamaguchi et al.
  • Organizer: The 23rd Naito Conference,Molecular Basis for Maintenance and Differentiation of Stem Cells [III]
  • Place of Presentation: Shonan Village Center, Kanagawa, Japan
• [Presentation] Six1 and Six4 Homeoproteins are required for Sex Determination in Mice (2008)
  • Author(s): Satomi S. Tanaka, Yuka Fujimoto, Yasuka L. Yamaguchi, Hiroki Kobayashi and Ryuichi Nishinakamura
  • Organizer: International symposium for gonad and brain sex differentiation
  • Place of Presentation: LAL resort Sea Hawk hotel Fukuoka, Fukuoka
• [Presentation] Dullard is essential for primordial germ cell formation and posterior extraembryonic mesoderm differentiation in the gastrulating mouse embryo (2008)
  • Author(s): Satomi S. Tanaka, Akihiro Nakane, Yasuka Y. Yamaguchi, Sayoko Fujimura, Makoto Asashima, Ryuichi Nishinakamura
  • Organizer: Annual meeting for the Japanese Society of Developmental Biologists (jointly sponsored by International Society of Developmental Biologists)
  • Place of Presentation: Tokushima Arts Foundation for Culture, Tokushima
• [Presentation] Stage-specific Importin13 activity regulates meiosis of germ cells in the mouse (2008)
  • Author(s): Yasuka L. Yamaguchi et al.
  • Organizer: Annual meeting for the Japanese Society of Developmental Biologists(jointly sponsored by International Society of Developmental Biologists)
  • Place of Presentation: Tokushima Arts Foundation for Culture, Tokushima
• [Presentation] The down regulation of gene-activity to study of oocyte development by tissue culture and knock down assay with siRNA. (2007)
  • Author(s): Yasuka L Yamaguchi
  • Organizer: 7th Transgenic Technology Meeting
  • Place of Presentation: オーストラリア、クィーンズランド大学
  • Year and Date: 2007-02-13
• [Presentation] マウス生殖細胞発生におけるSall4遺伝子の分子機構の解析 (2007)
  • Author(s): Yasuka L. Yamaguchi et al.
  • Organizer: 80th Annual Meeting for the Japanese Biochemical Society(Biochemistry and Molecular Biology 2007, BMB2007)
  • Place of Presentation: Pacifico Yokohama, Yokohama, Japan
• [Presentation] マウス生殖細胞形成・分化におけるSall4遺伝子の分子機構の解析 (2007)
  • Author(s): Yasuka L. Yamaguchi et al.
  • Organizer: 40th Annual Meeting for the Japanese Society of Developmental Biologists 2007(JSDB2007)
  • Place of Presentation: Fukuoka Dome, Fukuoka, Japan
• [Presentation] Sall4 is required for mouse primordial germ cell development
  • Author(s): Yasuka L. Yamaguchi, Satomi S. Tanaka, Ryuichi Nishinakamura
  • Organizer: COE Mini Symposium, February 19th 2009
  • Place of Presentation: Kumamoto Univ., Kumamoto. oral presenter
• [Book] Analyzing gene function in whole mouse embryo and fetal organ in vitro
  • Author(s): Satomi S. Tanaka, Yasuka L. Yamaguchi, Vanessa J. Jones and Patrick P. L. Tam.
  • Publisher: In Methods in Molecular Biology (M. Lewandoski ed.), Humana Press