High-throughput functional sceening and characterization of useful genotypes regulated-genes in planta

• Project/Area Number: 19780002
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Breeding science
• Research Institution: Tohoku University
• Principal Investigator: TAKAHASHI Yoshihiro Tohoku University, 大学院・生命科学研究科, 助教 (20390891)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,730,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥330,000); Fiscal Year 2008: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2007: ¥2,300,000 (Direct Cost: ¥2,300,000)
• Keywords: 植物分子育種 / 過剰発現 / 発現抑制 / 有用形質 / 環境ストレス / 耐病性

Research Abstract

本研究の目的は、植物ウイルスの能力を利用した迅速な過剰発現/発現抑制スクリーニング法を用いて植物の有用形質制御因子を網羅的に探索すると共に、単離した遺伝子の詳細な機能解明を行うことである。このスクリーニングにより、過剰発現又は発現抑制した際に致死となる因子、葉の形態形成を制御している因子、植物体の大きさを制御している因子、葉色を制御している因子、環境ストレス耐性に関与する因子等を網羅的に単離し、一部の遺伝子に関しては詳細な特徴付けを行った。

Research Products

• [Journal Article] Serine palmitoyltransferase, the first step enzyme in sphingolipid biosynthesis, is involved in nonhost resistance (2009)
  • Author(s): Takahashi, Y., Berberich, T., Kanzaki, H., Matsumura, H., Saitoh, H., Kusano, T. and Terauchi, R.
  • Journal Title: Mol. Plant-Microbe Interact 22 Pages: 31-38
  • Peer Reviewed
• [Journal Article] Plant voltage-dependent anion channels are involved in host defense against Pseudomonas cichorii and Bax-induced cell death (2009)
  • Author(s): Tateda, C., Yamashita, K., Takahashi, F., Kusano, T. and Takahashi, Y.
  • Journal Title: Plant Cell Rep 28 Pages: 41-51
  • Peer Reviewed
• [Journal Article] Serine palmitoyltransferase, the first step enzyme in sphingolipid biosynthesis, is involved in nonhost resistance (2009)
  • Author(s): Takahashi, et.al.
  • Journal Title: Molecular Plant-Microbe Interactions 22 Pages: 31-38
  • Peer Reviewed
• [Journal Article] Plant voltage-dependent anion channels are involved in host defense against Pseudomonas cichorii and Bax-induced cell death (2009)
  • Author(s): Tateda, et.al.
  • Journal Title: Plant Cell Reports 28 Pages: 41-51
  • Peer Reviewed
• [Journal Article] NtbZIP60, an endoplasmic reticulum-localized transcription factor, plays a role in the defense response against bacterial pathogens in Nicotiana tabacum (2008)
  • Author(s): Tateda, C., Ozaki, R., Onodera, Y., Takahashi, Y., Yamaguchi, K., Berberich, T., Koizumi, N. and Kusano, T.
  • Journal Title: J. Plant Res 121 Pages: 603-611
  • NAID: 10024447416
  • Peer Reviewed
• [Journal Article] High-throughput in planta expression screening identifies an ADP-ribosylation factor (ARF1) that is involved in non-host resistance and R gene mediated resistance (2008)
  • Author(s): Coemans, B., Takahashi, Y., Berberich, T., Ito, A., Kanzaki, H., Matsumura, H., Saitoh, H., Tsuda, S., Kamoun, S., Sagi, L., Swennen, R. and Terauchi, R.
  • Journal Title: Mol. Plant Pathol 9 Pages: 25-36
  • Peer Reviewed
• [Journal Article] NtbZIP60, an endoplasmic reticulum-localized transcription factor, plays a role in the defense response against bacterial pathogens in Nicotiana tabacum (2008)
  • Author(s): Tateda, et.al.
  • Journal Title: Journal of Plant Research 121 Pages: 603-611
  • Peer Reviewed
• [Journal Article] High-throughput in planta expression screening identifies an ADP-ribosylation factor (ARF1) involved in non-host resistance and Rgene-mediated resistance (2008)
  • Author(s): Coemans, et. al.
  • Journal Title: Molecular Plant Pathology 9 Pages: 25-36
  • Peer Reviewed
• [Presentation] Role for plant VDACs in plant innate immune systems (2008)
  • Author(s): Tateda, C., Kusano, T. and Takahashi, Y.
  • Organizer: International Conference on Biotic Plant Interactions
  • Place of Presentation: ブリスベン,オーストラリア
• [Presentation] Serine palmitoyltransferase is involved in pathogen defense response in Nicotiana benthamiana (2008)
  • Author(s): Takahashi, Y., Berberich, T., Kanzaki, H., Matsumura, H., Saitoh, H., Kusano, T. and Terauchi, R
  • Organizer: 18^<th> International Symposium on Plant Lipids
  • Place of Presentation: ボルドー,フランス
• [Presentation] Plant mitochondrial porin regulates defense response against bacterial pathogen and Bax-induced cell death (2008)
  • Author(s): Tateda, C., Kusano, T. and Takahashi, Y.
  • Organizer: Plant Neurobiology 2008
  • Place of Presentation: 九州大学
• [Presentation] 新規細胞死誘導因子bLCB2は病原体抵抗性反応に関与する (2008)
  • Author(s): 高橋芳弘,Berberich Thomas,神崎洋之,松村英生,齋藤宏昌,草野友延,寺内良平
  • Organizer: 日本農芸化学会
  • Place of Presentation: 名城大学
• [Presentation] スペルミン誘導性転写因子bZIP60の機能解析 (2008)
  • Author(s): 高橋芳弘,小野寺悠,舘田知佳,尾崎玲,Berberich Thomas,小泉望,草野友延
  • Organizer: 日本ポリアミン研究会
  • Place of Presentation: 崇城大学
• [Presentation] Serine palmitoyltransferase is involved in pathogen defense response in Nicotiana benthamiana (2008)
  • Author(s): Takahashi, et.al.
  • Organizer: 18^<th>International Symposium on Plant Lipids
  • Place of Presentation: ボルドー, フランス
• [Presentation] 新規細胞死誘導因子NbLCB2は病原体抵抗性反応に関与する (2008)
  • Author(s): 高橋 芳弘, ら
  • Organizer: 日本農芸化学会
  • Place of Presentation: 名城大学
• [Presentation] バクテリア抵抗性反応に関与するSerine Palmitoyltransferaseの機能解析 (2007)
  • Author(s): 高橋芳弘,Berberich Thomas,神崎洋之,松村英生,齋藤宏昌,草野友延,寺内良平
  • Organizer: 日本植物学会東北支部第20回秋田大会
  • Place of Presentation: 秋田県立大学
• [Book] The Handbook of Plant Functional Genomics. Edited by Gunter Kahl and Khalid Melsem, Chapter 6. High-throughput functional screening of genes in planta (2008)
  • Author(s): Berberich, T., Takahashi, Y., Saitoh, H. and Terauchi, R.
  • Publisher: WILEY-VCH Verlag GmbH & Co. KGaA
• [Book] The Handbook of Plant Functional Genomics. Edited by Granter Kahl and Khalid Melsem Chapter 6. High-throughput functional screening of genes in planta (2008)
  • Author(s): Berberich, et.al.
  • Publisher: WILEY-VCH Verlag GmbH & Co. KGaA
• [Remarks]
  • URL: http://www.ige.tohoku.ac.jp/outou/index-j.html