Positional cloning of phosphorus-deficiency-responsive traits in rice and its functional analyses for agriculture of environmental conservation type

• Project/Area Number: 19780006
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Breeding science
• Research Institution: The University of Shiga Prefecture
• Principal Investigator: SHIMIZU Akifumi The University of Shiga Prefecture, 環境科学部, 助教 (40409082)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥2,960,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥360,000); Fiscal Year 2009: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2008: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2007: ¥1,400,000 (Direct Cost: ¥1,400,000)
• Keywords: 植物育種 / 遺伝 / 環境保全型農業 / ストレス応答 / リン欠乏 / 環境修復 / 遺伝解析 / 遺伝育種学 / ストレス耐性 / ホスファターゼ / QTL解析 / イネ

Research Abstract

Deficiency of phosphorus which is one of the three major nutrients, is a limiting factor of agronomical production over the world. I have made progress in genetic analysis of two kinds of agronomical traits in rice, which can be useful for solving the problem. One trait is "Root Elongation under Phosphorus deficiency". Its contribution to P-deficiency tolerance was evaluated and fine-mapping of the gene was conducted. The other trait is "Acid Phosphatase" which can be useful for P recycling from old leaf blade. Its effect on P content in new developing leaf and the genetic factor were studied.

Research Products

• [Journal Article] Rapid construction of a high-density rice linkage map by High Efficiency Genome Scanning (HEGS) system. (2009)
  • Author(s): Shimizu A., Kawasaki S.
  • Journal Title: Rice Science 16 Pages: 247-251
  • Peer Reviewed
• [Journal Article] QTL analysis of genetic tolerance to iron-toxicity in rice (Oryza sativa L.) by quantification of bronzing score. (2009)
  • Author(s): Shimizu A.
  • Journal Title: Journal of New Seeds 10 Pages: 171-179
  • Peer Reviewed
• [Journal Article] QTL analysis of genetic tolerance to iron-toxicity in rice (Oryza sativa L. ) by quantification of bronzing score. (2009)
  • Author(s): Shimizu A.
  • Journal Title: Journal of New Seeds 10 Pages: 171-179
  • Peer Reviewed
• [Journal Article] QTL analysis of genetic tolerance to iron-toxicity in rice (Oryza sativa L.) by quantification of bronzing score. (2009)
  • Author(s): Shimizu, Akifumi
  • Journal Title: Journal of New Seeds 10 Pages: 171-179
  • Peer Reviewed
• [Journal Article] Rapid canstruction of a high-density rice linkage map by High Efficiency Genome Scanning (HEGS) system. (2009)
  • Author(s): Shimizu, A., Kawasaki S.
  • Journal Title: Rice Science 16 Pages: 247-251
  • Peer Reviewed
• [Journal Article] Genetic analysis of root elongation induced by phosphorus deficiency in rice (Oryza sativa L.) : fine QTL mapping and multivariate analysis of related traits (2008)
  • Author(s): Shimizu A., Kato K., Komatsu A., Motomura K., Ikehashi H.
  • Journal Title: Theoretical and Applied Genetics 117 Pages: 987-996
  • Peer Reviewed
• [Journal Article] Genetic analysis of root elongation induced by phosphorus deficiency in rice (Oryza sativa L. ): fine QTL mapping and multivariate analysis of related traits (2008)
  • Author(s): Shimizu A., Kato K., Komatsu A., Motomura K., Ikehashi H.
  • Journal Title: Theoretical and Applied Genetics 117 Pages: 987-996
  • Peer Reviewed
• [Journal Article] Genetic analysis of root elongation induced by phosphorus deficiency in rice (Oryza sativa L.): fine QTL mapping and multivariate analysis of related traits (2008)
  • Author(s): Akifumi Shimizu
  • Journal Title: Theoretical and Applied Genetics 117 Pages: 987-996
  • Peer Reviewed
• [Journal Article] Genome and transcriptome analyses for acid tolerance in rice:phosp horous deficiency and low pH stress. (2008)
  • Author(s): Shimizu, A.
  • Journal Title: Proceedings of the Final International Meeting for 21th Century Center for Excellence (COE) Program "Development of New Bioremediation Systems of Acid Sulfate Soil for Agriculture and Forestry" Pages: 25-28
• [Presentation] イネ根表面のiron plaque形成に関する突然変異体スクリーニング (2009)
  • Author(s): 松本公佑, ら
  • Organizer: 日本育種学会
  • Place of Presentation: 北海道大学(北海道)
  • Year and Date: 2009-09-25
• [Presentation] イネのリン欠乏ストレス耐性・低pH耐性のQTL解析と育種への展望 (2009)
  • Author(s): 清水顕史
  • Organizer: 日本植物細胞分子生物学会
  • Place of Presentation: 日本大学生物資源科学部(神奈川県)
  • Year and Date: 2009-07-31
• [Presentation] リン欠乏ストレス下におけるイネ葉身中のリン再利用 (2009)
  • Author(s): 清水顕史, ら
  • Organizer: 日本育種学会
  • Place of Presentation: つくば国際会議場(茨城県)
  • Year and Date: 2009-03-28
• [Presentation] リン欠乏ストレス下におけるイネ葉身中のリン再利用 (2009)
  • Author(s): 清水顕史
  • Organizer: 日本育種学会
  • Place of Presentation: つくば国際会議場
  • Year and Date: 2009-03-28
• [Presentation] グラフィカルモデルによる低リン・ストレス関連形質の多変量解析 (2008)
  • Author(s): 清水顕史
  • Organizer: 日本育種学会
  • Place of Presentation: 滋賀県立大学(滋賀県)
  • Year and Date: 2008-10-11
• [Presentation] グラフィカルモデルによる低リン・ストレス関連形質の多変量解析 (2008)
  • Author(s): 清水顕史
  • Organizer: 日本育種学会
  • Place of Presentation: 滋賀県立大学
  • Year and Date: 2008-10-11
• [Presentation] イネのリン酸欠乏によって誘導される根伸長3.精密QTLマッピングと発現遺伝子解析 (2007)
  • Author(s): 清水顕史, ら
  • Organizer: 日本育種学会
  • Place of Presentation: 山形大学(山形県)
  • Year and Date: 2007-09-22
• [Presentation] イネのリン酸欠乏によって誘導される根伸長3. 精密QTLマッピングと発現遺伝子解析 (2007)
  • Author(s): 清水顕史, ら
  • Organizer: 日本育種学会
  • Place of Presentation: 山形大学(山形県)
  • Year and Date: 2007-09-22
• [Presentation] イネのリン酸欠乏によって誘導される根伸長3.精密QTLマッピングと発現遺伝子解析 (2007)
  • Author(s): 清水 顕史
  • Organizer: 日本育種学会
  • Place of Presentation: 山形大学
  • Year and Date: 2007-09-22
• [Book] Genome and transcriptome analyses for acid tolerance in rice : phosphorous deficiency and low pH stress.(Development of New Bioremediation Systems of Acid Sulfate Soil for Agriculture and Forestry.)(S.Sasaki (eds)) ISBN 978-4-87974-608-5 (2008)
  • Author(s): Shimizu A., Kato K., Komatsu A., Ikehashi H.
  • Publisher: SHOUKADOH Book Sellers, Kyoto
• [Book] Development of New Bioremediation Systems of Acid Sulfate Soil for Agriculture and Forestry. (S. Sasaki (eds), Genome and transcriptome analyses for acid tolerance in rice: phosphorous deficiency and low pH stress.) (2008)
  • Author(s): Shimizu A., Kato K., Komatsu A., Ikehashi H.
  • Publisher: SHOUKADOH Book Sellers