Development of host rice as a bioreacter for high value recombinant protein production

• Project/Area Number: 19780011
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Breeding science
• Research Institution: National Institute of Agrobiological Sciences
• Principal Investigator: KAWAKATU Taiji National Institute of Agrobiological Sciences, 遺伝子組換え作物開発センター, 任期付研究員 (30435614)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥3,520,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥420,000); Fiscal Year 2009: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2008: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2007: ¥1,700,000 (Direct Cost: ¥1,700,000)
• Keywords: イネ / 胚乳 / 種子貯蔵タンパク質 / 転写因子 / 遺伝子発現制御 / リジン / アミノ酸含量 / 外来遺伝子産物高蓄積

Research Abstract

This research project aims for understanding mechanisms of seed storage protein (SSP) synthesis in rice endosperm. We generated several transgenic rice plants with reduced SSPs, in which recombinant products can use surplus amino acids. Rice seed specific transcription factors RISBZ1 and RPBF regulate SSP gene expression redundantly. RISBZ1 and RPBF are regulatory factors of endosperm quality.

Research Products

• [Journal Article] The 3'-untranslated region of rice glutelin GluB-1 affects accumulation of heterologous protein in transgenic rice. (2009)
  • Author(s): Yang L, Wakasa Y, Kawakatsu T, Takaiwa F
  • Journal Title: Biotechnology Letters 31 Pages: 1625-1631
• [Journal Article] Compensation and interaction between RISBZ1 and RPBF during grain filling in rice. (2009)
  • Author(s): Kawakatsu T, Yamamoto M. P, Touno S. M, Yasuda H, Takaiwa F
  • Journal Title: The Plant Journal 59 Pages: 908-920
  • NAID: 130006995167
• [Journal Article] Overexpression of BiP has inhibitory effects on the accumulation of seed storage proteins in endosperm cells of rice. (2009)
  • Author(s): Yasuda H, Hirose S, Kawakatsu T, Wakasa Y, Takaiwa F
  • Journal Title: Plant and Cell Physiology 50 Pages: 1532-1543
  • NAID: 10027342743
• [Journal Article] PLASTOCHRON3/GOLIATH encodes a glutamate carboxypeptidase required for proper development in rice (2009)
  • Author(s): Kawakatsu T, Taramino G, Itoh J, Allen J, Sato Y, Hong S-K, Yule R, Nagasawa N, Kojima M, Kusaba M, Sakakibara H, Sakai H, Nagato Y
  • Journal Title: The Plant Journal 58 Pages: 1028-1040
• [Journal Article] Overexpression of BiP has inhibitory effects on the accumulation of seed storage proteins in endosperm cells of rice (2009)
  • Author(s): Yasuda H, Hirose S, Kawakatsu T, Wakasa Y, Takaiwa F
  • Journal Title: Plant and Cell Physiology 50 Pages: 1532-1543
  • NAID: 10027342743
  • Peer Reviewed
• [Journal Article] Compensation and interaction between RISBZ1 and RPBF during grain filling in rice (2009)
  • Author(s): Kawakatsu T, Yamamoto M.P, Touno S.M, Yasuda H, Takaiwa F
  • Journal Title: The Plant Journal 59 Pages: 908-920
  • NAID: 130006995167
  • Peer Reviewed
• [Journal Article] The 3'-untranslated region of rice glutelin GluB-1 affects accumulation of heterologous protein in transgenic rice (2009)
  • Author(s): Yang L, Wakasa Y, Kawakatsu T, Takaiwa F
  • Journal Title: Biotechnology Letters 31 Pages: 1625-1631
  • Peer Reviewed
• [Journal Article] Characterization of a new rice glutelin gene GluD-1 expressed in the starchy endosperm. (2008)
  • Author(s): Kawakatsu T, Yamamoto M. P, Hirose S, Yano M, Takaiwa F
  • Journal Title: Journal of Experimental Botany 59 Pages: 4233-4245
• [Journal Article] スギ花粉症緩和米の安全性確保への取り組み-大規模隔離ほ場栽培と生物多様性影響評価- (2008)
  • Author(s): 廣瀬咲子, 高木英典, 川勝泰二, 若佐雄也, 土門英司, 遠藤雄士, 村岡賢一, 平井一夫, 渡邊朋也, 服部誠, 立石剣, 高岩文雄
  • Journal Title: 育種学研究 10 Pages: 23-30
  • NAID: 110006633703
• [Journal Article] Characterization of a new rice glutelin gene GluD-1 expressed in the starchy endosperm. (2008)
  • Author(s): Kawakatsu, et al.
  • Journal Title: Journal of Experimental Botany 59 Pages: 4233-4245
  • Peer Reviewed
• [Journal Article] スギ花粉症緩和米の安全性確保への取り組み-大規模隔離ほ場栽培と生物多様性影響評価- (2008)
  • Author(s): 廣瀬咲子
  • Journal Title: 育種学研究 10 Pages: 23-30
  • Peer Reviewed
• [Presentation] イネbZIP型転写因子RISBZ1とDOF型転写因子RPBFの種子登熟における補償作用および相互作用 (2010)
  • Author(s): 川勝泰二
  • Organizer: 日本植物生理学会
  • Place of Presentation: 熊本大学(熊本)
  • Year and Date: 2010-03-21
• [Presentation] Compensation and interaction between RISBZ1 and RPBF during grain filling in rice. (2009)
  • Author(s): Kawakatsu T
  • Organizer: the 9th International Plant Molecular Biology Congress
  • Place of Presentation: セントルイス(アメリカ)
  • Year and Date: 2009-10-27
• [Presentation] The role of ER-chaperone BiP on quality control in rice endosdperm cells (2009)
  • Author(s): Wakasa Y
  • Organizer: the 9th International Plant Molecular Biology Congress
  • Place of Presentation: セントルイス(アメリカ)
  • Year and Date: 2009-10-27
• [Presentation] Compensation and interaction between RISBZ1 and RPBF during grain filling in rice (2009)
  • Author(s): 川勝泰二, 山本将之, 東野(百瀬)悟, 保田浩, 高岩文雄
  • Organizer: the 9th International Plant Molecular Biology Congress
  • Place of Presentation: セントルイス(アメリカ)
  • Year and Date: 2009-10-27
• [Presentation] イネbZIP型転写因子RISBZ1とDOF型転写因子RPBFの種子登熟における補償作用および相互作用 (2009)
  • Author(s): 川勝泰二
  • Organizer: 日本育種学会
  • Place of Presentation: 北海道大学(北海道)
  • Year and Date: 2009-09-26
• [Presentation] イネ胚乳タンパク質の品質管理におけるERシャペロンBiPの役割 (2009)
  • Author(s): 若佐雄也
  • Organizer: 日本育種学会
  • Place of Presentation: 北海道大学(北海道)
  • Year and Date: 2009-09-26
• [Presentation] RSISを用いた新規遺伝子発現抑制手法の開発 (2009)
  • Author(s): 高岩文雄
  • Organizer: 日本育種学会
  • Place of Presentation: 北海道大学(北海道)
  • Year and Date: 2009-09-26
• [Presentation] 内胚乳で発現するイネグルテリン遺伝子GluD-1の発現制御機構の解析 (2009)
  • Author(s): 川勝泰二
  • Organizer: 日本育種学会
  • Place of Presentation: つくば国際会議場(茨城県)
  • Year and Date: 2009-03-28
• [Presentation] 内胚乳で発現するイネグルテリン遺伝子GluD-1の発現制御機構の解析 (2009)
  • Author(s): 川勝 泰二, 高岩 文雄
  • Organizer: 日本育種学会第115回講演会
  • Place of Presentation: つくば国際会議場
  • Year and Date: 2009-03-28
• [Presentation] イネ種子貯蔵タンパク質低減系統の作出および解析 (2008)
  • Author(s): 川勝泰二
  • Organizer: 日本育種学会
  • Place of Presentation: 滋賀県立大学(滋賀県)
  • Year and Date: 2008-10-12
• [Presentation] イネ種子貯蔵タンパク質低減系統の作出および解析 (2008)
  • Author(s): 川勝 泰二, 他3名
  • Organizer: 日本育種学会第114回講演会
  • Place of Presentation: 滋賀県立大学
  • Year and Date: 2008-10-12
• [Presentation] イネ新規グルテリン遺伝子GluD-1の同定および解析 (2007)
  • Author(s): 川勝泰二
  • Organizer: 日本育種学会
  • Place of Presentation: 山形大学(山形県)
  • Year and Date: 2007-09-23
• [Presentation] イネ新規グルテリン遺伝子GluD-1の同定・解析 (2007)
  • Author(s): 川勝泰二
  • Organizer: 日本育種学会 第112回講演会
  • Place of Presentation: 山形大学
  • Year and Date: 2007-09-23
• [Patent(Industrial Property Rights)] 植物の内胚乳に特異的に発現する遺伝子および該遺伝子のプロモーター、並びにそれらの利用 (2009)
  • Inventor(s): 高岩文雄、川勝泰二
  • Industrial Property Rights Holder: 農業生物資源研究所
  • Filing Date: 2009-04-10
• [Patent(Industrial Property Rights)] 植物の内胚乳に特異的に発現する遺伝子および該遺伝子のプロモーター、並びにそれらの利用 (2009)
  • Inventor(s): 高岩文雄、川勝泰二
  • Industrial Property Rights Holder: 農業生物資源研究所
  • Filing Date: 2009-04-10
  • Overseas
• [Patent(Industrial Property Rights)] 植物の内胚乳に特異的に発現する遺伝子および該遺伝子のプロモーター、並びにそれらの利用 (2008)
  • Inventor(s): 高岩文雄、川勝泰二
  • Industrial Property Rights Holder: 農業生物資源研究所
  • Industrial Property Number: 2008-104132
  • Filing Date: 2008-04-11
• [Patent(Industrial Property Rights)] 植物の内胚に特異的に発現する遺伝子および該遺伝子のプロモター、並びにそれらの利用 (2008)
  • Inventor(s): 高岩 文雄, 川勝 泰二
  • Industrial Property Number: 2008-104132
  • Filing Date: 2008-04-11