SSR analysis of genetic variability of herbicide-resistant weed populations

• Project/Area Number: 19780014
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Crop science/Weed science
• Research Institution: Kyoto Prefectural University
• Principal Investigator: OHSAKO Takanori Kyoto Prefectural University, 生命環境科学研究科, 講師 (80363969)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥3,930,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥630,000); Fiscal Year 2009: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2007: ¥1,200,000 (Direct Cost: ¥1,200,000)
• Keywords: 水田雑草 / スルホニルウレア系除草剤 / 除草剤抵抗性突然変異 / SSR(マイクロサテライト) / 遺伝的多様性 / イヌホタルイ / オモダカ / SSR (マイクロサテライト)

Research Abstract

SSR markers were constructed for two paddy weed species, Schoenoplectus juncoides and Sagitarria trifolia. Population genetic surveys were conducted for sulfonylurea-resistant and susceptible populations of these species using SSR markers.

Research Products

• [Journal Article] Clonal and spatial genetic structure within populations of a coastal plant Carex kobomugi (Cyperaceae). (2010)
  • Author(s): Takanori Ohsako
  • Journal Title: American Journal of Botany 97 Pages: 458-470
• [Journal Article] Clonal and spatial genetic structure within populationsof a coastal plant, Carex kobomugi (Cyperaceae) (2010)
  • Author(s): Takanori Ohsako
  • Journal Title: American Journal of Botany 97 Pages: 458-470
  • Peer Reviewed
• [Journal Article] Nucleotide sequence variability of the Adh gene of the coastal plant Calystegia soldanella (Convolvulaceae) in Japan. (2008)
  • Author(s): Takanori Ohsako, Gakuto Matsuoka
  • Journal Title: Genes and Genetic Systems 83 Pages: 89-94
  • NAID: 10024395687
• [Journal Article] Genetic diversity of sulfonylurea-resistant and -susceptible Monochoria vaginalis (Burm. f. ) Kunth populations in Japan. (2008)
  • Author(s): Toshiyuki Imaizumi, Guang-Xi Wang, Takanori Ohsako, Tohru Tominaga
  • Journal Title: Weed Research 48 Pages: 187-196
• [Journal Article] Microsatellite variability within and among local landrace populations of tea, Camellia sinensis (L. ) O. Kuntze, in Kyoto, Japan. (2008)
  • Author(s): Takanori Ohsako, Takafumi Ohgushi, Hino Motosugi, Kouzou Oka
  • Journal Title: Genetic Resources and Crop Evolution 55 Pages: 1047-1053
• [Journal Article] Self-EcoTILLING to identify single-nucleotide mutations in multigene family. (2008)
  • Author(s): Guang-Xi Wang, Toshiyuki Imaizumi, Wei Li, Hiromasa Saitoh, Ryohei Terauchi, Takanori Ohsako, Tohru Tominaga
  • Journal Title: Pesticide Biochemistry and Physiology 92 Pages: 24-29
• [Journal Article] Microsatellite variability within and among local landrace populations of tea, Camellia sinensis (L.) O. Kuntze, in Kyoto, Japan (2008)
  • Author(s): Takanori Ohsako, Takafumi Ohgushi, Hino Motosugi and Kouzou Oka
  • Journal Title: enetic Resources and Crop Evolution 55 Pages: 1047-1053
  • Peer Reviewed
• [Journal Article] Self-EcoTILLING to identify single-nucleotide mutations in multigene family (2008)
  • Author(s): Guang-Xi Wang, Toshiyuki Imaizumi, Wei Li, Hiromasa Saitoh, Ryohei Terauchi, Takanori Ohsako and Tohru Tominaga
  • Journal Title: Pesticide Biochemistry and Physiology 92 Pages: 24-29
  • Peer Reviewed
• [Journal Article] Nucleotide sequence variability of the Adh gene of the coastal plant Calystegia soldanella(Convolvulaceae)in Japan (2008)
  • Author(s): Takanori Ohsako and Gakuto M atsuoka
  • Journal Title: Genes and Genetic Systems 83 Pages: 89-94
  • NAID: 10024395687
  • Peer Reviewed
• [Journal Article] Isolation and characterization of polymorphic microsatellite loci in Asiatic sand sedge Carex kobomugi Ohwi(Cyperaceae) (2007)
  • Author(s): Takanori Ohsako and Kyoko Ya mane
  • Journal Title: Molecular Ecology Notes 7 Pages: 1023-1025
  • Peer Reviewed
• [Journal Article] Nucleotide substitutions in the acetolactate synthase genes of sulfonylurea-resistant biotypes of Monochoria vaginalis(Pontederiaceae) (2007)
  • Author(s): Takanori Ohsako and Tohru To minaga
  • Journal Title: Genes and Genetic Systems 82 Pages: 207-215
  • NAID: 10024394330
  • Peer Reviewed
• [Presentation] ハマダイコン野生集団内および集団間におけるマイクロサテライト変異の解析 (2009)
  • Author(s): 大迫敬義、山吹誠
  • Organizer: 日本育種学界第116回講演会
  • Place of Presentation: 北海道大学
  • Year and Date: 2009-09-26
• [Presentation] 海浜植物コウボウムギCarex kobomugi (Cyperaceae)のクローン構造および空間遺伝構造 (2008)
  • Author(s): 大迫敬義
  • Organizer: 日本育種学会第114回講演会
  • Place of Presentation: 滋賀県立大学
  • Year and Date: 2008-10-11
• [Presentation] 海浜植物コウボウムギCarex kobomugi(Cyperaceae)のクローン構造および空間遺伝構造 (2008)
  • Author(s): 大迫敬義
  • Organizer: 日本育種学会第114回講演会
  • Place of Presentation: 滋賀県立大学
  • Year and Date: 2008-10-11
• [Presentation] ハマヒルガオ野生集団におけるアルコール脱水素酵素遺伝子の塩基配列変異 (2007)
  • Author(s): 大迫敬義、松岡岳人
  • Organizer: 日本育種学会第112回講演会
  • Place of Presentation: 山形大学農学部
  • Year and Date: 2007-09-22