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Functional analysis of petal senescence-related genes using RNAi method

Research Project

Project/Area Number 19780019
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Horticulture/Landscape architecture
Research InstitutionTokyo University of Agriculture and Technology

Principal Investigator

YAMADA Tetsuya  Tokyo University of Agriculture and Technology, 大学院・共生科学技術研究院, 講師 (20422511)

Project Period (FY) 2007 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥3,630,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2008: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2007: ¥2,200,000 (Direct Cost: ¥2,200,000)
Keywordsアサガオ / プログラム細胞死 / 液胞プロセッシング酵素 / アポトーシス / 花弁老化 / 老化関連遺伝子 / RNAi / オートファジー
Research Abstract

In this study, we evaluated effects of knockout of petal senescence-relatedgenes, InVPE, InPSR26, InPSR29 and InPSR42, using RNAi method on petal senescence and showedthat two genes of them, InPSR26 and InPSR29, are carrying out a function to suppress senescence in thepetals of Japanese morning glory. The function of the other two genes, InVPE and InPSR42, could notbe clarified, but homology search of these genes suggested that they are deeply related to theprogrammed cell death in the petals of morning glory.

Report

(4 results)
  • 2009 Annual Research Report   Final Research Report ( PDF )
  • 2008 Annual Research Report
  • 2007 Annual Research Report
  • Research Products

    (23 results)

All 2010 2009 2008 2007 Other

All Journal Article (12 results) (of which Peer Reviewed: 10 results) Presentation (10 results) Remarks (1 results)

  • [Journal Article] 植物器官のデジタル画像面積を効率的に計測できる領域抽出ソフトウエアの開発2010

    • Author(s)
      七夕高也, 山田哲也, 清水悠介, 篠崎良仁, 金勝一樹, 高野誠
    • Journal Title

      園芸学研究 (印刷中)

    • NAID

      130004476560

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] 植物器官のデジタル画像面積を効率的に計測できる領域抽出ソフトウエアの開発2010

    • Author(s)
      七夕高也
    • Journal Title

      園芸学研究 (印刷中)

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] InPSR26 encoding a putative membrane protein is involved in programmed cell death during petal senescence of Japanese morning glory (Ipomoea nil)2009

    • Author(s)
      Shibuya K, Yamada T, Shimizu K, Ichimura K
    • Journal Title

      Acta Horticulturae 847

      Pages: 275-278

    • Related Report
      2009 Final Research Report
  • [Journal Article] Homologues of genes associated with programmed cell death in animal cells are differentially expressed during senescence of Ipomoea nil petals2009

    • Author(s)
      Yamada T, Ichimura K, Kanekatsu M, van Doorn WG
    • Journal Title

      Plant Cell Physiology 50

      Pages: 610-625

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] InPSR26, a putative membrane protein, regulates programmed cell death during petal senescence in Ipomoea nil2009

    • Author(s)
      Shibuya K, Yamada T, Suzuki T, Shimizu K, Ichimura K
    • Journal Title

      Plant Physiology 149

      Pages: 816-824

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] InPSR26 encoding a putative membrane protein is involved in programmed cell death during petal senescence of Japanese morning glory(Ipomoea nil)2009

    • Author(s)
      Shibuya
    • Journal Title

      Acta Horticulturae 847

      Pages: 275-278

    • Related Report
      2009 Annual Research Report
  • [Journal Article] InPSR26, a putative membrane protein, regulates programmed cell death during petal senescence in Ipomoea nil2009

    • Author(s)
      Kenichi Shibuya
    • Journal Title

      Plant Physiology 149

      Pages: 816-824

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Homologues of genes associated with programmed cell death in animal cells are differentially expressed during senescence of Ipomoea nil petals2009

    • Author(s)
      Tetsuya Yamada
    • Journal Title

      Plant & Cell Physiology 50

      Pages: 610-625

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Relationship between petal abscission and programmed cell death in Prunus yedoensis and Delphinium belladonna2007

    • Author(s)
      Yamada T, Ichimura K, van Doorn WG
    • Journal Title

      Planta 226

      Pages: 1195-1205

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Gene expression in opening and senescing petals of morning glory (Ipomoea nil) flowers2007

    • Author(s)
      Yamada T, Ichimura K, Kanekatsu M, van Doorn WG
    • Journal Title

      Plant Cell Report 26

      Pages: 823-835

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Gene expression in opening and senescing petals of morning glory(Ipomoea nil) flowers2007

    • Author(s)
      Tetsuya Yamada
    • Journal Title

      Plant Cell Reports 26

      Pages: 823-835

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Relationship between petal abscission and programmed cell death in Prunus yedoensis and Delpyinium belladonna2007

    • Author(s)
      Tetsuya Yamada
    • Journal Title

      Planta 226

      Pages: 1195-1205

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Presentation] アサガオの花弁老化におけるオートファジーと老化関連遺伝子の発現との関係2010

    • Author(s)
      篠崎良仁
    • Organizer
      園芸学会平成22年度春季大会
    • Place of Presentation
      日本大学(神奈川県)
    • Year and Date
      2010-03-21
    • Related Report
      2009 Annual Research Report
  • [Presentation] アサガオの花弁老化におけるオートファジーと老化関連遺伝子の発現との関係2010

    • Author(s)
      篠崎良仁, 山田哲也, 金勝一樹
    • Organizer
      園芸学会平成22年度春季
    • Place of Presentation
      日本大学
    • Related Report
      2009 Final Research Report
  • [Presentation] 膜貫通型LRRプロテインキナーゼ遺伝子のホモログIn29はアサガオの花弁老化に関与する2009

    • Author(s)
      李マイ
    • Organizer
      日本育種学会第116回講演会
    • Place of Presentation
      北海道大学(北海道)
    • Year and Date
      2009-09-26
    • Related Report
      2009 Annual Research Report
  • [Presentation] 膜貫通型LRRプロテインキナーゼ遺伝子のホモログIn29はアサガオの花弁老化に関与する2009

    • Author(s)
      李マイ, 鈴木智子, 清水圭一, 七夕高也, 篠崎良仁, 高橋彩佳, 渋谷健市, 市村一雄, 山田哲也, 金勝一樹
    • Organizer
      日本育種学会第116回
    • Place of Presentation
      北海道大学
    • Related Report
      2009 Final Research Report
  • [Presentation] アサガオ花弁における老化関連遺伝子の発現量の系統間差異2008

    • Author(s)
      篠崎良仁
    • Organizer
      日本育種学会第114回講演会
    • Place of Presentation
      滋賀県立大学
    • Year and Date
      2008-10-12
    • Related Report
      2008 Annual Research Report
  • [Presentation] アサガオの膜タンパク質をコードするInPSR26遺伝子は花弁老化時のプログラム細胞死に関与している2008

    • Author(s)
      渋谷健一
    • Organizer
      園芸学会平成20年度秋季大会
    • Place of Presentation
      三重大学
    • Year and Date
      2008-09-28
    • Related Report
      2008 Annual Research Report
  • [Presentation] アサガオ花弁における老化関連遺伝子の発現量の系統間差異2008

    • Author(s)
      篠崎良仁, 山田哲也, 七夕高也, 宮村浩子, 斎藤隆文, 金勝一樹
    • Organizer
      日本育種学会第114回
    • Place of Presentation
      滋賀県立大学
    • Related Report
      2009 Final Research Report
  • [Presentation] アサガオの膜タンパク質をコードするInPSR26遺伝子は花弁老化時のプログラム細胞死に関与している2008

    • Author(s)
      渋谷健一, 山田哲也, 鈴木智子, 清水圭一, 市村一雄
    • Organizer
      園芸学会平成20年度秋季
    • Place of Presentation
      三重大学
    • Related Report
      2009 Final Research Report
  • [Presentation] 葉の老化関連転写因子NAPの遺伝子ホモログは老化誘導期のアサガオ花弁で発現量が著しく増加する2008

    • Author(s)
      篠崎良仁, 山田哲也, 金勝一樹
    • Organizer
      日本育種学会第113回
    • Place of Presentation
      明治大学
    • Related Report
      2009 Final Research Report 2007 Annual Research Report
  • [Presentation] RNAi法によるアサガオのアントシアニン合成酵素遺伝子の発現抑制2007

    • Author(s)
      鈴木智子, 山田哲也, 清水圭一, 市村一雄, 金勝一樹
    • Organizer
      日本育種学会第112回
    • Place of Presentation
      山形大学
    • Related Report
      2009 Final Research Report 2007 Annual Research Report
  • [Remarks] 開発したソフトウエアのサイト

    • URL

      http://www.kazusa.or.jp/picasos/

    • Related Report
      2009 Final Research Report

URL: 

Published: 2007-04-01   Modified: 2016-04-21  

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