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Effect of mycorrhizal growth stimulatory compounds on spore production and biocontrol of soil-borne plant pathogens in fruit orchards

Research Project

Project/Area Number 19780022
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Horticulture/Landscape architecture
Research InstitutionKyoto Prefectural University

Principal Investigator

CRUZ Andre Freire (CRUZ A・F / CRUZ AF)  Kyoto Prefectural University, 生命環境科学研究科, 講師 (80405240)

Project Period (FY) 2007 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥2,900,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2008: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2007: ¥1,600,000 (Direct Cost: ¥1,600,000)
Keywords菌根菌 / 細菌 / 物質 / 生物防除 / 果樹栽培 / 部室 / 内在菌 / 土壌病原菌 / 科学分析
Research Abstract

Three kinds of bacteria were isolated from arbuscular mycorrhizal spores and identified as Bacillus sp., Bacillus thuringiensis, and Paenibacillus rhizospherae. These bacteria also functioned on biocontrol of soil-borne plant pathogens Fusarium oxysporum, Rosellinia necatrix, Rhizoctonia solani and Pythium ultimum. Moreover, they were effective on phosphorus solubilization, ethylene production, nitrogenase activity, and stimulation of mycorrhizae hyphal growth in vitro. In addition, fresh samples of spores and hyphae were photographed using an in situ scanning electron microscope, where some bacterial slimes were detected on hyphae and spores.

Report

(5 results)
  • 2009 Annual Research Report   Final Research Report ( PDF )
  • 2008 Annual Research Report
  • 2007 Annual Research Report
  • Products Report
  • Research Products

    (16 results)

All 2012 2011 2010 2009 2008 Other

All Journal Article (2 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (13 results) (of which Int'l Joint Research: 1 results,  Invited: 1 results) Remarks (1 results)

  • [Journal Article] Arbuscular mycorrhizal fungal spores host bacteria that affect nutrient biodynamics and biocontrol of soil‐borne plant pathogens2012

    • Author(s)
      Cruz. A. F, Ishii. T
    • Journal Title

      Biology Open

      Volume: 1 Issue: 1 Pages: 52-57

    • DOI

      10.1242/bio.2011014

    • Related Report
      Products Report
    • Peer Reviewed / Open Access
  • [Journal Article] Sod culture management contributes to sustainable fruit growing through propagation of arbuscular mycorrhizal fungi and their helper-microorganisms, and decreasing of agricultural inputs2010

    • Author(s)
      Cruz. A. F, Ishii. T
    • Journal Title

      Acta horticulturae

      Volume: 933 Issue: 933 Pages: 477-484

    • DOI

      10.17660/actahortic.2012.933.62

    • Related Report
      Products Report
  • [Presentation] Response of trifoliate orange seedlings to combined inoculation of an arbuscular mycorrhizal fungus ; Gigaspora margarita Becker and Hall, and its partner bacteria2011

    • Author(s)
      Cruz. A. F, M. Yoneda, H.Matsubara, T.Ishii
    • Organizer
      日本園芸学会
    • Place of Presentation
      宇都宮大学 (栃木県宇都宮市)
    • Year and Date
      2011-03-20
    • Related Report
      Products Report
  • [Presentation] Mycorrhizal Spores Harbor Functional Bacteria on Disease Suppression and Nutrient Biodynamics2010

    • Author(s)
      Cruz. A. F
    • Organizer
      VI Symposium Nacional y Tercera Reunion Iberoamericana de la Simbiosis Micorrizica (招待講演) (国際学会)
    • Place of Presentation
      Universidad Autónoma De Tlaxcala, México
    • Year and Date
      2010-09-06
    • Related Report
      Products Report
    • Int'l Joint Research / Invited
  • [Presentation] Bacteria isolated from the spores of an arbuscular mycorrhizal fungus, Gigaspora margarita, stimulate its hyphal growth, and have the abilities of P solubilization and nitrogen fixation.2010

    • Author(s)
      Cruz, A.F., K. Takemori, K. Shimizu, M. Yoneda, T. Ishii.
    • Organizer
      日本園芸学会
    • Place of Presentation
      神奈川県藤沢市-日本大学
    • Year and Date
      2010-03-21
    • Related Report
      2009 Final Research Report
  • [Presentation] Bacteria isolated from the spores of an arbuscular mycorrhizal fungus, Gigaspora margarita, stimulate its hyphal growth, and have the abilities of Psolubilization and nitrogen fixation2010

    • Author(s)
      Cruz,A.F., K.Takemori, K.Shimizu, M.Yoneda, T.Ishii
    • Organizer
      日本園芸学会
    • Place of Presentation
      神奈川県-日本大学
    • Related Report
      2009 Annual Research Report
  • [Presentation] Antagonism of bacteria living inside or on the surface of arbuscular mycorrhizal fungus, Gigaspora margarita spores to the growth of Roselinia necatrix: a serious soil-borne pathogen in Japanese fruit orchards.2009

    • Author(s)
      Cruz, A.F., K. Takemori, Y. Toda, T. Ishii.
    • Organizer
      日本園芸学会
    • Place of Presentation
      秋田県秋田市-秋田大学
    • Year and Date
      2009-09-26
    • Related Report
      2009 Final Research Report
  • [Presentation] Bacteria living inside and/or on surface of Gigaspora margarita spores and their effects on soil-borne plant diseases and P solubilization.2009

    • Author(s)
      Cruz A.F., Takemori, K., Toda Y., Horii, S., Ishii T.
    • Organizer
      6th International congress on mycorrhiza. 国際学会
    • Place of Presentation
      Belo Horizonte-Brazil.
    • Year and Date
      2009-08-09
    • Related Report
      2009 Final Research Report
  • [Presentation] Isolation of bacteria living inside or on surface of the spores of an arbuscular mycorrhizal fungus, Gigaspora margarita, and their antagonistic effects on soil-borne plant diseases.2009

    • Author(s)
      Takemori, K., Y. Toda, K. Hayashida, D. Yu, A.F. Cruz, T. Ishii.
    • Organizer
      日本園芸学会
    • Place of Presentation
      東京都千代田区-明治大学
    • Year and Date
      2009-03-19
    • Related Report
      2009 Final Research Report
  • [Presentation] Isolation of bacteria living inside or on surface of the spores of anarbuscular mycorrhizal fungus, Gigaspora margarita, and their antagonisticeffects on soil-borne plant diseases2009

    • Author(s)
      Takemori,K., Y.Toda, K.Hayashida, D.Yu, A.F.Cruz, T.Ishii
    • Organizer
      日本園芸学会
    • Place of Presentation
      東京-明治大学
    • Related Report
      2009 Annual Research Report
  • [Presentation] Bacteria living inside and/or on surface of Gigaspora margarita spores and their effects on soil-borne plant diseases and Psolubilization2009

    • Author(s)
      Cruz A.F., Takemori,K., Toda Y., Horii, S., Ishii T.
    • Organizer
      6^<th> International congress on mycorrhiza
    • Place of Presentation
      ブラジル国-ベロオリゾンテ市
    • Related Report
      2009 Annual Research Report
  • [Presentation] Antagonism of bacteria living inside or on the surface of azbuscular mycorrhizal fungus, Gigaspora margarita spores to the growth of Roselinia necatrix : a serious soil-borne pathogen in Japanese fruit orchazds2009

    • Author(s)
      Cruz,A.F., K.Takemori, Y.Todaand T.Ishii
    • Organizer
      日本園芸学会
    • Place of Presentation
      秋田県-秋田大学
    • Related Report
      2009 Annual Research Report
  • [Presentation] Isolation of bacteria living inside or on surface of the spores of an arbuscular mycorrhizal fungus, Gigaspora margarita, and their antagonistic effects on soil-borne plant diseases2009

    • Author(s)
      Takemori, K., Y. Toda, K. Hayash ida, D. Yu, A. F. Cruz and T. Ishii
    • Organizer
      日本園芸学会
    • Place of Presentation
      東京-明治大学
    • Related Report
      2008 Annual Research Report
  • [Presentation] アーバスキュラー菌根菌、Gigaspora margaritaの胞子に内在する細菌が土壌病原菌の生物的防除およびG. margaritaの生長促進に及ぼす影響2008

    • Author(s)
      クルス アンドレ フレイリ・堀井幸江・三輪由佳・落合彩織・石井孝昭
    • Organizer
      日本園芸学会
    • Place of Presentation
      神奈川県本厚木市-東京農業大学
    • Year and Date
      2008-03-31
    • Related Report
      2009 Final Research Report
  • [Presentation] アーバスキュラー菌根菌、Gigaspora margaritaの胞子に内在する細菌が土壌病原菌の生物的防除およびG.margaritaの生長促進に及ぼす影響2008

    • Author(s)
      クルス アンドレ フレイリ・堀井幸江・三輪由佳・落合彩織・石井孝昭
    • Organizer
      日本園芸学会
    • Place of Presentation
      神奈川県本厚木市
    • Year and Date
      2008-03-31
    • Related Report
      2007 Annual Research Report
  • [Remarks]

    • URL

      http://eureka.kpu.ac.jp/~andre/2017/index.html

    • Related Report
      2009 Final Research Report

URL: 

Published: 2007-04-01   Modified: 2018-04-26  

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