A study on pathogenicity-determining structures of polygalacturonases from Geotrichum candidum by using chimera proteins

• Project/Area Number: 19780037
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Plant pathology
• Research Institution: Kagoshima University
• Principal Investigator: NAKAMURA Masayuki Kagoshima University, 農学部, 助教 (90404475)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥1,940,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥240,000); Fiscal Year 2008: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2007: ¥900,000 (Direct Cost: ¥900,000)
• Keywords: ポリガラクツロナーゼ / プロトペクチナーゼ / 白かび病菌 / 病原性 / 白かび病 / キメラタンパク / Geotrichum candidum

Research Abstract

カンキツ果実に病気を引き起こす白かび病菌(Geotrichum candidum)には、病気を起こさない非病原性株が存在する。この病原性の違いに、植物細胞壁分解酵素の1種であるポリガラクツロナーゼ(PG)のプロトペクチナーゼ活性(自然界で植物組織内に存在している不溶性ペクチンを分解する活性)が関わっていることを明らかにした。これまで、PGが病原性に関わっているかどうか不明な点が多く、論争が起きていたが、それを解決する大きな糸口となった。

Research Products

• [Journal Article] Establishment of heterologous expression of polygalacturonase S63PG1 from nonpathogenic isolate S63 of Geotrichum candidum (2009)
  • Author(s): Nakamura M, Nakamura K, Iwai H
  • Journal Title: Journal of General Plant Pathology (in press)
  • NAID: 10029718192
  • Peer Reviewed
• [Journal Article] Establishment of heterologous expression of polygalacturonase S63PG1 from nonpathogenic isolate S63 of Geotrichum candidum (2009)
  • Author(s): Nakamura M, Nakamura K, Iwai H.
  • Journal Title: Journal of General Plant Pathology (印刷中)
  • NAID: 10029718192
  • Peer Reviewed
• [Journal Article] Differentiation of pathogenic and nonpathogenic isolates of Geotrichum candidum sensu Suprapta et al. (1995) on citrus fruit based on PCR-RFLP analysis of rDNA ITS and PCR using specific primers designed in polygalacturonase genes (2008)
  • Author(s): Nakamura M, Suprapta DN, Iwai H
  • Journal Title: Mycoscience 49 Pages: 155-158
  • NAID: 10021182570
  • Peer Reviewed
• [Journal Article] Differentiation of pathogenic and nonpathogenic isolates of Geotrichum candidum sensu Suprapta, et. al. (1995) on citrus fruit based on PCR-RFLP analysis of rDNA ITS and PCR using specific primers designed inpolygalac turonase genes (2008)
  • Author(s): Nakamura M・Suprapta D. N・Iwai H
  • Journal Title: Mycoscience 49 Pages: 155-158
  • Peer Reviewed
• [Presentation] Geotrichum candidum S63(非病原性株)由来ポリガラクツロナーゼの分裂酵母を用いた外分泌系の確立 (2008)
  • Author(s): 中村格朗・中村正幸・岩井久
  • Organizer: 九州病害虫研究会
  • Place of Presentation: メルパルク熊本
  • Year and Date: 2008-01-31
• [Presentation] Protopectinase activity of polygalacturonases from pathogenic and nonpathogenic isolates of Geotrichum cadidum governs their pathogenicity to citrus fruit (2008)
  • Author(s): Nakamura M, Nakamura K, Iwai H
  • Organizer: 9th International Congress of Plant Pathology
  • Place of Presentation: Torino, Italy
• [Presentation] Protopectinase activity of polygalacturonases from pathogenic and nonpathogenic isolates of Geotrichum cadidum governs theirpathogenicity tocitrus fruit (2008)
  • Author(s): Nakamura M, Nakamura K, Iwai H.
  • Organizer: 9^<th> International Congress of Plant Pathology
  • Place of Presentation: Torio, Italy